Format:
XV, 329 S.
,
Ill., graph. Darst.
,
23 cm
ISBN:
1588299139
,
9781588299130
Series Statement:
Methods in molecular biology 703
Note:
Literaturangaben
,
The transcriptional landscape
,
Working with RNA
,
Synthesis of RNA by in vitro transcription
,
Efficient poly(A)+ RNA selection using LNA oligo(T) capture
,
Genome browsers
,
Web-based tools for studying RNA structure and function
,
Northern blotting analysis
,
Rapid amplification of cDNA ends (RACE)
,
Fractionation of mRNA based on the length of the poly(A) tail
,
Analysis of mutations that influence pre-mRNA splicing
,
S1 nuclease analysis of alternatively spliced mRNA
,
Promises and challenges in developing RNAi as a research tool and therapy
,
Inhibition of gene function in mammalian cells using short-hairpin RNA (shRNA)
,
Validation of RNAi by real time PCR
,
Profiling RNA polymerase II using the fast chromatin immunoprecipitation method
,
The post-transcriptional operon
,
RIP-chip analysis : RNA-binding protein immunoprecipitation-microarray (chip) profiling
,
Isolation of RNP granules
,
Electrophoretic mobility shift assay for characterizing RNA-protein interaction
,
Polysome analysis and RNA purification from sucrose gradients
,
Prediction of targets for microRNAs
,
Outsourcing of experimental work
Additional Edition:
ISBN 9781597452489
Additional Edition:
Online-Ausg. Nielsen, Henrik RNA Totowa, NJ : Humana Press, 2011 ISBN 9781597452489
Language:
English
Subjects:
Biology
Keywords:
Messenger-RNS
;
Labortechnik
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