Adenoviral vaccine induction of CD8+ T cell memory inflation: Impact of co-infection and infection order
Fig 3
Sequential infection with Ad-LacZ followed by MCMV.
C57BL/6 mice were first immunized with Ad-lacZ, then >50 days later were infected with MCMV. The size of the pre-existing Ad-lacZ specific M38 effector memory response after MCMV infection was measured in the blood and liver by ex-vivo tetramer staining. (A) The percentage of pre-existing inflating D8V tetramer+ population in the blood and (B) the absolute numbers in the liver after MCMV infection was determined at the indicated time points. (C) The kinetics and magnitude of the new inflating response (M38) in groups of mice previously immunized with Ad-lacZ was also measured by ex vivo tetramer staining and compared against uninfected mice. The percentage of M38 tetramer+ cells in the blood and (D) the absolute numbers in the liver was measured at the indicated time points. The figures show the mean from 3–8 mice per time point obtained from 2 independent experiments. p values were measured by Mann-Whitney tests followed by Dunn’s multiple comparison. *p<0.05, **p<0.005.