Format:
Online-Ressource (XLV, 373 S.)
,
Ill., graph. Darst.
Edition:
Online-Ausg. 2011 Electronic reproduction; Available via World Wide Web
ISBN:
9780123851123
,
1283101300
Series Statement:
Methods in enzymology 494
Content:
Produced by microbes on a large scale, methane is an important alternative fuel as well as a potent greenhouse gas. This volume focuses on microbial methane metabolism, which is central to the global carbon cycle. Both methanotrophy and methanogenesis are covered in detail. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state of the art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state of the art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field.
Note:
Literaturangaben
,
Front Cover; Methods in Methane Metabolism, Part A: Methanogenesis; Copyright; Contents; Contributors; Preface; Methods In Enzymology; Chapter 1: Techniques for Cultivating Methanogens; 1. Introduction; 2. Methanogens on a Budget; 3. Use of an Anoxic Chamber; Acknowledgments; References; Chapter 2: Genetic Methods for Methanosarcina Species; 1. Introduction; 2. Liposome-Mediated Transformation; 3. Deleting Genes; 4. Test for Gene Essentiality; 5. Adding Genes; Acknowledgments; References; Chapter 3: Genetic Systems for Hydrogenotrophic Methanogens; 1. Introduction; 2. Genome Sequences
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3. Growth and Storage of Methanogen Cultures4. Genetic Tools; 5. Transformation Methods; 6. Other Genetic Techniques; Acknowledgments; References; Chapter 4: Molecular Tools for Investigating ANME Community Structure and Function; 1. Introduction; 2. Exploring ANME Population Structure; 3. Detecting ANME Proteins in Marine Sediments; Acknowledgments; References; Chapter 5: Studying Gene Regulation in Methanogenic Archaea; 1. Introduction; 2. Quantitative Reverse Transcription PCR for M. maripaludis; 3. Analyzing Gene Expression Using uidA Reporter Gene Fusions in M. acetivorans
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4. Creating Random Disruption Mutants of M. maripaludis by In Vitro Transposon MutagenesisAcknowledgments; References; Chapter 6: Growth of Methanogens Under Defined Hydrogen Conditions; 1. Introduction; 2. Hydrogen Limitation in Batch Culture; 3. Hydrogen Limitation in Continuous Culture; Acknowledgments; References; Chapter 7: Preparation of [Fe]-Hydrogenase from Methanogenic Archaea; 1. Introduction; 2. Oxygen, Light, and Copper Sensitivity of [Fe]-Hydrogenase and FeGP Cofactor; 3. Preparation of [Fe]-Hydrogenase; 4. Preparation of FeGP Cofactor
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5. Preparation of Reconstituted [Fe]-Hydrogenase6. Assay of the Enzyme Activity; Acknowledgments; References; Chapter 8: Assay of Methylotrophic Methyltransferases from Methanogenic Archaea; 1. Introduction; 2. Preparation of Anoxic Cell Extracts of M. Barkeri; 3. Isolation and Resolution of MtmBC Complexes; 4. Ellman's Assay of Methylotrophic Substrate: CoM Methyl Transfer; 5. Assay of Methylthiol:CoM Methyltransferases; 6. Assay of Methylotrophic Substrate:Corrinoid Methyltransferase Activity; 7. Spectral Assay of Methylcobalamin:CoM Methyltransferases; Acknowledgments; References
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Chapter 9: Methyl-Coenzyme M Reductase from Methanothermobacter Marburgensis1. Introduction; 2. Equipment; 3. Cell Growth: Basic Procedure; 4. Whole-Cell Experiments; 5. Cell Growth: Induction of Different Forms of MCR; 6. Enzyme Purification; 7. Different MCR Forms; 8. Materials; Acknowledgments; References; Chapter 10: Methods for Analysis of Acetyl-CoA Synthase: Applications to Bacterial and Archaeal Systems; 1. Introduction; 2. Assays of A Cluster Protein (ACS) Catalytic Activities; 3. Acetyltransferase Reactions; 4. Acetyl-CoA Synthesis Reactions
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5. The Acetyl-CoA Carbonyl:CO Exchange Reaction
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Electronic reproduction; Available via World Wide Web
In:
Pt. A
Additional Edition:
ISBN 0691081042
Additional Edition:
Print version Methods in Methane Metabolism Part A : Methanogenesis
Language:
English
Keywords:
Methangärung
;
Methanstoffwechsel
;
Aufsatzsammlung
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