Format:
Online-Ressource (XXXI, 681 S.)
Edition:
Online-Ausg.
ISBN:
9780121820862
Series Statement:
Methods in enzymology 185
Content:
The methods presented in this volume will enable the reader to design effective strategies for the expression of cloned genes and cDNAs and will prove useful in solving the majority of expression problems one is likely to encounter
Note:
Includes bibliographical references
,
Includes indexes
,
Expression of heterologous genes in mammalian cells
,
Heterologous gene expression in yeast
,
Systems for heterologous gene expression
,
Expression of heterologous genes in mammalian cells
,
Heterologous gene expression in yeast
,
Systems for heterologous gene expression
,
Literaturangaben
,
Vectors used for expression in mammalian cells
,
Assembly of enhancers, promoters, and splice signals to control expression of transferred genes
,
Methods for introducing DNA into mammalian cells
,
Selection and coamplification of heterologous genes in mammalian cells
,
Optimizing cell and culture environment for production of recombinant proteins
,
Analysis of synthesis, processing, and secretion of proteins expressed in mammalian cells
,
Saturation mutagenesis using mixed oligonucleotides and M13 templates containing uracil
,
Propagation and expression of cloned genes in yeast: 2-[mu]m circle-based vectors
,
Manipulating yeast genome using plasmid vectors
,
Regulated GAL4 expression cassette providing controllable and high-level output from high-copy galactose promoters in yeast
,
Expression of heterologous proteins in Saccharomyces cerevisiae using the ADH2 promoter
,
Induced expression using yeast copper metallothionein promoter
,
High-efficiency yeast expression vectors based on the promoter of the phosphoglycerate kinase gene
,
Glyceraldehyde-3-phosphate dehydrogenase-derived expression cassettes for constitutive synthesis of heterologous proteins
,
Superimposition of temperature regulation on yeast promoters
,
Sequence and structural requirements for efficient translation in yeast
,
Vacuolar proteases in yeast Saccharomyces cerevisiae
,
Detection and inhibition of ubiquitin-dependent proteolysis
,
Contranslational amino-terminal processing
,
[alpha]-Factor leader-directed secretion of heterologous proteins from yeast
,
Use of heterologous and homologous signal sequences for secretion of heterologous proteins from yeast
,
Isolation, characterization, and properties of Saccharomyces cerevisiae mnn mutants with nonconditional protein glycosylation defects
,
Molecular and genetic approach to enhancing protein secretion
,
Expression of heterologous proteins in escherichia coli
,
Design and construction of expression plasmid vectors in escherichia coli
,
Promoter recognition and mRNA initiation by Escherichia coli E[sigma]70
,
Use of Escherichia coli trp promoter for direct expression of proteins
,
Use of T7 RNA polymerase to direct expression of cloned genes
,
High-level translation initiation
,
Enhanced translational efficiency with two-cistron expression system
,
Sequences within ribosome binding site affecting messenger RNA translatability and method to direct ribosomes to single messenger RNA species
,
Vector for enhanced translation of foreign genes in Escherichia coli
,
Minimizing proteolysis in Escherichia coli: genetic solutions
,
Gene fusions for purpose of expression: An introduction
,
Fusions to staphylococcal protein A
,
Expression as trpE fusion
,
Engineering Escherchia coli to secrete heterologous gene products
,
Refolding of recombinant proteins
,
Expression of heterologous genes in Bacillus subtilis
,
Regulated promoter for high-level expression of heterologous genes in Bacillus subtilis
,
System for secretion of heterologous proteins in Bacillus subtilis
,
Inducible expression of regulatory genes in Bacillus subtilis
,
Vectors used for expression in mammalian cells
,
Assembly of enhancers, promoters, and splice signals to control expression of transferred genes
,
Methods for introducing DNA into mammalian cells
,
Selection and coamplification of heterologous genes in mammalian cells
,
Optimizing cell and culture environment for production of recombinant proteins
,
Analysis of synthesis, processing, and secretion of proteins expressed in mammalian cells
,
Saturation mutagenesis using mixed oligonucleotides and M13 templates containing uracil
,
Propagation and expression of cloned genes in yeast: 2-[mu]m circle-based vectors
,
Manipulating yeast genome using plasmid vectors
,
Regulated GAL4 expression cassette providing controllable and high-level output from high-copy galactose promoters in yeast
,
Expression of heterologous proteins in Saccharomyces cerevisiae using the ADH2 promoter
,
Induced expression using yeast copper metallothionein promoter
,
High-efficiency yeast expression vectors based on the promoter of the phosphoglycerate kinase gene
,
Glyceraldehyde-3-phosphate dehydrogenase-derived expression cassettes for constitutive synthesis of heterologous proteins
,
Superimposition of temperature regulation on yeast promoters
,
Sequence and structural requirements for efficient translation in yeast
,
Vacuolar proteases in yeast Saccharomyces cerevisiae
,
Detection and inhibition of ubiquitin-dependent proteolysis
,
Contranslational amino-terminal processing
,
[alpha]-Factor leader-directed secretion of heterologous proteins from yeast
,
Use of heterologous and homologous signal sequences for secretion of heterologous proteins from yeast
,
Isolation, characterization, and properties of Saccharomyces cerevisiae mnn mutants with nonconditional protein glycosylation defects
,
Molecular and genetic approach to enhancing protein secretion
,
Expression of heterologous proteins in escherichia coli
,
Design and construction of expression plasmid vectors in escherichia coli
,
Promoter recognition and mRNA initiation by Escherichia coli E[sigma]70
,
Use of Escherichia coli trp promoter for direct expression of proteins
,
Use of T7 RNA polymerase to direct expression of cloned genes
,
High-level translation initiation
,
Enhanced translational efficiency with two-cistron expression system
,
Sequences within ribosome binding site affecting messenger RNA translatability and method to direct ribosomes to single messenger RNA species
,
Vector for enhanced translation of foreign genes in Escherichia coli
,
Minimizing proteolysis in Escherichia coli: genetic solutions
,
Gene fusions for purpose of expression: An introduction
,
Fusions to staphylococcal protein A
,
Expression as trpE fusion
,
Engineering Escherchia coli to secrete heterologous gene products
,
Refolding of recombinant proteins
,
Expression of heterologous genes in Bacillus subtilis
,
Regulated promoter for high-level expression of heterologous genes in Bacillus subtilis
,
System for secretion of heterologous proteins in Bacillus subtilis
,
Inducible expression of regulatory genes in Bacillus subtilis
Additional Edition:
Erscheint auch als Druck-Ausgabe Gene expression technology San Diego, Calif. [u.a.] : Academic Press, 1990 ISBN 012287045X
Additional Edition:
ISBN 0121820866
Language:
English
Subjects:
Chemistry/Pharmacy
,
Biology
Keywords:
Genexpression
;
Mikroorganismus
;
Genexpression
;
Säugetiere
;
Genexpression
;
cDNS
;
Genexpression
;
Genklonierung
;
Genexpression
;
Genexpression
;
Technologie
;
Aufsatzsammlung
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