Format:
Online-Ressource (XII, 387 p. 76 illus., 41 illus. in color, digital)
ISBN:
9789400745346
,
1280994223
,
9781280994227
Series Statement:
SpringerLink
Content:
Systems Metabolic Engineering is changing the way microbial cell factories are designed and optimized for industrial production. Integrating systems biology and biotechnology with new concepts from synthetic biology enables the global analysis and engineering of microorganisms and bioprocesses at super efficiency and versatility otherwise not accessible. Without doubt, systems metabolic engineering is a major driver towards bio-based production of chemicals, materials and fuels from renewables and thus one of the core technologies of global green growth. In this book, Christoph Wittmann and Sang-Yup Lee have assembled the world leaders on systems metabolic engineering and cover the full story from genomes and networks via discovery and design to industrial implementation practises. This book is a comprehensive resource for students and researchers from academia and industry interested in systems metabolic engineering. It provides us with the fundaments to targeted engineering of microbial cells for sustainable bio-production and stimulates those who are interested to enter this exiting research field.
Note:
Description based upon print version of record
,
; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; Systems Metabolic Engineering; Preface; Contents; Chapter 1: Genome-Scale Network Modeling; 1.1 Introduction; 1.2 Metabolic Networks; 1.2.1 Metabolic Network Case Studies; 1.3 Transcriptional Networks; 1.3.1 Transcriptional Network Case Studies; 1.4 Cell Signaling Networks; 1.4.1 Signaling Network Case Studies; 1.5 Concluding Remarks; References; Chapter 2: Kinetic Modeling of Metabolic Networks; 2.1 Introduction
,
2.2 Modeling Chemical Reaction Dynamics2.2.1 Defining the Scope of a Metabolic Model; 2.2.2 Dynamic Models of Metabolism; 2.2.3 The Law of Mass Action and Reaction Thermodynamics; 2.2.4 Phenomenological Rate Laws; 2.2.5 Enzyme Kinetics; 2.3 Analyzing Metabolic Network Dynamics; 2.3.1 The Stoichiometric Matrix; 2.3.2 Formation and Dynamics of Aggregate Variables; 2.3.3 Linear Analysis; 2.4 Towards Large-Scale Kinetic Models of Metabolism; 2.4.1 Iterative Model Construction; 2.4.2 Data Fitting; 2.4.3 Construction of Kinetic Models via High-Throughput Data Mapping
,
2.4.4 Parameter Sensitivity and Random Sampling2.4.5 Numerical Considerations; 2.5 Closing: Practical Applications of Kinetic Models; References; Chapter 3: Design of Superior Cell Factories Based on Systems Wide Omics Analysis; 3.1 Introduction; 3.2 Transcriptome Analysis for Breeding of Superior Cell Factories; 3.2.1 Transcriptome Analysis; 3.2.2 Comparative Transcriptome Analysis; 3.2.3 Relationship Between Transcriptome and Phenome Data; 3.2.4 Transcriptomics in Evolutionary Engineering; 3.3 Multi-omics Analyses for Breeding of Superior Cell Factories
,
3.3.1 Proteome, Metabolome, Fluxome Anlaysis3.3.2 Integrative Multi-omics Analysis; 3.4 Summary; References; Chapter 4: Technologies for Biosystems Engineering; 4.1 Introduction; 4.2 Fundamentals of Gene Synthesis; 4.3 From Genes to the Assembly of Genomes; 4.3.1 In Vitro Approaches; 4.3.2 In Vivo Assembly of DNA Fragments; 4.3.3 Assembly of Long DNA Stretches up to Entire Genomes; 4.4 Tools for (Semi-) Rational and Combinatorial Biosystems Engineering; 4.4.1 Promoters, Ribosome Binding Sites, Terminators, and RNA Tools; 4.4.1.1 Promoters; 4.4.1.2 Ribosome Binding Sites
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4.4.1.3 Transcriptional Terminators4.4.1.4 RNA-Tools; 4.4.2 Design Software and Registries; 4.5 Engineering and Streamlining Genomes; 4.5.1 Engineering the Chromosome of E. coli: Deletions, Insertions and Modifications; 4.5.1.1 Methods for General Genome Modifications; 4.5.1.2 Methods for Site-Specific In-Frame Deletions; 4.5.2 Streamlining Microbial Hosts; 4.6 Summary; References; Chapter 5: Systems Metabolic Engineering of Escherichia coli for Chemicals, Materials, Biofuels, and Pharmaceuticals; 5.1 Introduction; 5.2 Chemicals and Materials; 5.2.1 Spider Silk Protein
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5.2.2 Polyhydroxyalkanoate
Additional Edition:
ISBN 9789400745339
Additional Edition:
Buchausg. Systems metabolic engineering Dordrecht : Springer, 2012 ISBN 9400745338
Additional Edition:
ISBN 9789400745339
Language:
English
Subjects:
Biology
Keywords:
Biotechnologie
;
Industrielle Mikrobiologie
;
Biokonversion
;
Systembiologie
;
Aufsatzsammlung
DOI:
10.1007/978-94-007-4534-6
URL:
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