Format:
Online-Ressource
ISSN:
1521-4141
Content:
Abstract: The activities of 8 S and 6 S DNA ligases have been studied in the chicken lymphoid cells of blood, spleen and bursa of Fabricius at different stages of development, from late embryonic life to about 3 months after hatching. These cells have been sorted with the fluorescence‐activated cell sorter FACS Il on the basis of size and T or B antigenicity (immunofluorescence). The light 6S DNA ligase has been previously demonstrated to be associated to a late stage of differentiation of thymocytes. In the bursa, a unique form of 8 S DNA ligase is found during the whole period of observation. This form of enzyme remains in the B cells of the spleen until 3 weeks after hatching, but is never present in the blood B cells. As far as T cells are concerned, the light DNA ligase is present in the blood from 18‐day embryonic life on. In the spleen T cells, on the contrary, this enzyme appears only 3 weeks after hatching. Before this stage, splenic T cells are devoid of any form of DNA ligase activity. These findings show biochemical differences in T and B lymphocytes colonizing the periphery, blood and spleen, and suggest, at least for the T cells at early stages, a heterogeneity in the degree of differentiation.
In:
volume:15
In:
number:1
In:
year:2005
In:
pages:77-82
In:
extent:6
In:
European journal of immunology, Weinheim : Wiley-VCH, 1971-, 15, Heft 1 (2005), 77-82 (gesamt 6), 1521-4141
Language:
English
DOI:
10.1002/eji.1830150115
URN:
urn:nbn:de:101:1-2024031906172989849758
URL:
https://doi.org/10.1002/eji.1830150115
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2024031906172989849758
URL:
https://d-nb.info/1323821910/34
URL:
https://doi.org/10.1002/eji.1830150115
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