Format:
Online-Ressource (LXI, 862 p. 188 illus., 187 illus. in color, online resource)
ISBN:
9781461432098
Series Statement:
Advances in Experimental Medicine and Biology 801
Content:
This book will contain the proceedings of the XV International Symposium on Retinal Degeneration (RD2012). A majority of those who will speak and present posters at the meeting will contribute to this volume. The blinding diseases of inherited retinal degenerations have no treatments, and age-related macular degeneration has no cures, despite the fact that it is an epidemic among the elderly, with 1 in 3-4 affected by the age of 70. The RD Symposium will focus on the exciting new developments aimed at understanding these diseases and providing therapies for them. Since most major scientists in the field of retinal degenerations attend the biennial RD Symposia, they are known by most as the “best” and “most important” meetings in the field. The volume will present representative state-of-the-art research in almost all areas of retinal degenerations, ranging from cytopathologic, physiologic, diagnostic and clinical aspects; animal models; mechanisms of cell death; candidate genes, cloning, mapping and other aspects of molecular genetics; and developing potential therapeutic measures such as gene therapy and neuroprotective agents for potential pharmaceutical therapy. While advances in these areas of retinal degenerations will be described, there will be many new topics that either were in their infancy or did not exist at the time of the last RD Symposium, RD2010. These include the role of inflammation and immunity, as well as other basic mechanisms, in age-related macular degeneration, several new aspects of gene therapy, and revolutionary new imaging and functional testing that will have a huge impact on the diagnosis and following the course of retinal degenerations, as well as to provide new quantitative endpoints for clinical trials. The retina is an approachable part of the central nervous system (CNS), and there is a major interest in neuroprotective and gene therapy for CNS diseases and neurodegenerations, in general. It should be noted that with successful and exciting initial clinical trials in neuroprotective and gene therapy, including the restoration of sight in blind children, the retinal degeneration therapies are leading the way towards new therapeutic measures for neurodegenerations of the CNS. Many of the successes recently reported in these areas of retinal degeneration sprang from collaborations established at previous RD Symposia, and many of those will be reported at the RD2010 meeting and included in the ...
Note:
Description based upon print version of record
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Preface; Travel Awards; Contents; Contributors; About the Editors; Part I; Basic Processes: Development, Physiology and Function; Chapter-1; Cell Type-Specific Epigenetic Signatures Accompany Late Stages of Mouse Retina Development; References; Chapter-2; Programmed Cell Death During Retinal Development of the Mouse Eye; 2.1 Introduction; 2.2 Programmed Cell Death in the Mouse Retina; 2.3 Neurotrophins; 2.4 Signaling Mechanisms During Programmed Cell Death of the Mouse Retina; References; Chapter-3; Spatial and Temporal Localization of Caveolin-1 Protein in the Developing Retina
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3.1 Introduction3.2 Methods; 3.3.2 Cav-1 Expression Increases Dramatically in Neuroretina as Müller glia Mature; 3.3 Results; 3.3.1 Cav-1 is Expressed by the Vasculature During Retinal Development; 3.4 Discussion; References; Chapter-4; Glutathione S-Transferase Pi Isoform (GSTP1) Expression in Murine Retina Increases with Developmental Maturity; 4.1 Introduction; 4.1.1 GSTP1 and Oxidative Stress; 4.1.2 GSTP1 and Maturation; 4.1.3 Light Toxicity; 4.2 Materials and Methods; 4.2.1 Experiment with Animals; 4.2.2 Light Exposure; 4.2.3 Immunohistochemistry; 4.2.4 Western Blot Analysis; 4.3 Results
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4.3.1 GSTP1 Expression in Retina Accompanied Murine Retinal Maturation4.3.2 GSTP1 Levels in Murine Retina Increased with Developmental Age and with Light Exposure; 4.4 Discussion; References; Chapter-5; RETINA-Specific Expression of Kcnv2 Is Controlled by Cone-Rod Homeobox (Crx) and Neural Retina Leucine Zipper (Nrl); 5.1 Introduction; 5.2 Materials and Methods; 5.2.1 Mouse Husbandry; 5.2.2 DNA Constructs; 5.2.3 RNA-Isolation, RT-PCR and qRT-PCR; 5.2.4 Bioinformatic Analysis; 5.2.5 Chromatin Immunoprecipitation; 5.2.6 Electroporation of Mouse Retinas; 5.3 Results
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5.3.1 Kcnv2 is Highly Expressed in the Murine Retina5.3.2 Kcnv2 Localizes to Inner Segment Membranes of Photoreceptors; 5.3.3 Retinal Kcnv2 Expression Is Regulated by Crx and Nrl; 5.4 Discussion; References; Chapter-6; AIPL1 Protein and its Indispensable Role in Cone Photoreceptor Function and Survival; 6.1 Introduction; 6.2 Animal Models of AIPL1 Deficiency; 6.3 Link Between AIPL1 and Rod PDE6; 6.4 AIPL1 in Mouse Cones; 6.5 AIPL1 in Primate Cones; 6.6 Conclusions and Future Directions; References; Chapter-7; Primate Short-Wavelength Cones Share Molecular Markers with Rods; 7.1 Introduction
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7.2 Materials and Methods7.2.1 Tissue; 7.2.2 Immunohistochemical (IHC) Staining; 7.3 Results; 7.3.1 Cone Arr4 (Pab LUMIf; Mab 7G6); 7.3.2 Rod Arr1 (S-Antigen; Mab D9F2; Pab C10C10); 7.3.3 Cone Alpha Transducin (CTr; Mab A1.1); 7.3.4 Rod Transducin (RTr; sc389); 7.3.5 Calbindin-D24k (CalB; Mab C8666); 7.4 Discussion; References; Chapter-8; Exploration of Cone Cyclic Nucleotide-Gated Channel-Interacting Proteins Using Affinity Purification and Mass Spectrometry; 8.1 Introduction; 8.2 Materials and Methods; 8.2.1 Animals, Antibodies, and Other Materials
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8.2.2 Antibody Affinity Purification, Mass Spectrometry, and Tandem Mass Spectrometry (MS/MS) Analyses
Additional Edition:
ISBN 9781461432081
Additional Edition:
Druckausg. ISBN 978-1-4614-3208-1
Language:
English
Subjects:
Medicine
Keywords:
Netzhautdegeneration
DOI:
10.1007/978-1-4614-3209-8
URL:
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