Format:
6
ISSN:
1095-9203
Content:
〈p〉Cellular membranes act as signaling platforms and control solute transport. Membrane receptors, transporters, and enzymes communicate with intracellular processes through protein-protein interactions. Using a split-ubiquitin yeast two-hybrid screen that covers a test-space of 6.4 × 10〈sup〉6〈/sup〉 pairs, we identified 12,102 membrane/signaling protein interactions from 〈i〉Arabidopsis〈/i〉. Besides confirmation of expected interactions such as heterotrimeric G protein subunit interactions and aquaporin oligomerization, 〉99% of the interactions were previously unknown. Interactions were confirmed at a rate of 32% in orthogonal in planta split-green fluorescent protein interaction assays, which was statistically indistinguishable from the confirmation rate for known interactions collected from literature (38%). Regulatory associations in membrane protein trafficking, turnover, and phosphorylation include regulation of potassium channel activity through abscisic acid signaling, transporter activity by a WNK kinase, and a brassinolide receptor kinase by trafficking-related proteins. These examples underscore the utility of the membrane/signaling protein interaction network for gene discovery and hypothesis generation in plants and other organisms.〈/p〉
Note:
Gesehen am 29.06.2021
In:
Science, Washington, DC : American Association for the Advancement of Science, 1880, 344(2014), 6185, Seite 711-716, 1095-9203
In:
volume:344
In:
year:2014
In:
number:6185
In:
pages:711-716
In:
extent:6
Language:
English
DOI:
10.1126/science.1251358
URL:
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