In:
Journal of Cell Science, The Company of Biologists, Vol. 98, No. 4 ( 1991-04-01), p. 545-550
Abstract:
Monoclonal antibodies have been developed against PC-1 protein, the major hydroxyproline-rich glycoprotein (HRGP) from maize pericarp, and have been used as probes in an indirect alkaline phosphatase antibody assay to localize this protein in tissue of three maize varieties. Kernels were sampled several days after pollination (d.a.p.). HRGPs were assayed by reacting antibodies with tissue prints of kernels and embedded tissue samples. Tissue prints show that soluble HRGPs are present in small quantities even at early stages of kernel development The greatest accumulation of PC-1 in the pericarp is at the top of the kernel. As the kernels mature, HRGPs begin to accumulate in the developing embryo. Total HRGPs in embedded tissue begin to accumulate at 10 d.a.p. and increase quite dramatically at 20-30 d.a.p. Popcorn kernels lose antigenic reactivity at 40 d.a.p., while sweet corn kernels continue to show strong reactivity.
Type of Medium:
Online Resource
ISSN:
0021-9533
,
1477-9137
DOI:
10.1242/jcs.98.4.545
Language:
English
Publisher:
The Company of Biologists
Publication Date:
1991
detail.hit.zdb_id:
219171-4
detail.hit.zdb_id:
1483099-1
SSG:
12
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