Kooperativer Bibliotheksverbund

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Format: 155 S.

ISBN: 9788773043455

Series Statement: Biologiske skrifter 57

Note: Literaturverz. S. [122] - 124

Language: English

Subjects: Biology

Keywords: Forsskål, Peter 1732-1763 ; Ägypten ; Arabisch ; Pflanzenname ; Expedition ; Geschichte 1761-1763 ; Jemen ; Pflanzen ; Herbarium

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Format: 1 Online-Ressource (XIV, 124 S)

Edition: Karger eBooks Collection

ISBN: 9783805590662 , 3805590660

Series Statement: BioValley monographs 3

Content: Genetically modified organisms (GMO) raise societal, political and ethical concerns. They inspire strong resistance or, conversely, enthusiastic assent. The aim of this publication is to give an overview of genetic engineering, starting with the history of the discovery of restriction enzymes continuing with technical aspects of transgenesis to its applications in research and ethical considerations. Be it the use of single engineered cells or GMO, these applications cover a broad array, ranging from disease-oriented research (but not only), to the promising perspectives of gene therapy. Historical and technical aspects give insights into the problems inherent to the creation of GMO, and illustrate the links and limits between genetic engineering, GMOs and gene therapy. A summary article in English and French structures the links between the different chapters and concepts.Scientists interested in genetic engineering of single cells or animal models, as well as in gene therapy, will find an up-to-date review on the use and perspectives of transgenesis. However, this publication is also recommended to the public interested in the definition of GMO, which encompasses a much broader array than the genetically modified crops covered by media

Note: New insights into the technical aspects and applications of transgenesis , Genetically modified organisms : concepts and methodsRestriction enzymes : from their discovery to their applications -- Transforming growth factor-beta superfamily : animal models for development and disease -- Transfection of human neuroblastoma cells with Alzheimer's Disease brain hallmarks as a promising strategy to investigate the role of neurosteroidogenesis in neuroprotection -- Investigating therapeutic strategies for Fragile X syndrome in mice -- Innovative therapeutic perspectives in neuromuscular diseases -- Gene therapy for cancer treatment : state of the art -- Manipulating the mitochondrial genetic system.

Additional Edition: ISBN 9783805590655

Language: English

Subjects: Biology

Keywords: Biomedizin ; Gentechnologie ; Electronic books ; Aufsatzsammlung

URL: Volltext

URL: lizenzpflichtig

URL: Inhaltsverzeichnis

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Format: Online-Ressource (XXI, 198 p. 75 illus., 71 illus. in color, online resource)

ISBN: 9789401785662

Series Statement: SpringerLink

Content: The oceans cover over 70% of our planet. They are host to a biodiversity of tremendous wealth. Its preservation is now a global priority featuring in several international conventions and a confirmed objective of European policies and national strategies. Understanding the dynamics and the uses of the marine biodiversity is a genuine scientific challenge. Fourteen international experts have got together and identified five priority research themes to address the problem, based on analysing the state of knowledge.

Content: Oceans cover more than 70% of earth and host a largely unknown marine biodiversity critical for future human beings. An improved knowledge of marine biodiversity at various scales (from genes to ecosystems) is required to facilitate building integrated scenarios and policies for a sustainable development as well as its conservation. Understanding species interactions, ecosystem functioning and dynamics, as well as biodiversity uses is presently a scientific challenge. Conservation of marine biodiversity is now a worldwide priority emphasized by several international conventions (UN, CBD.) and a key target for European policies and national strategies. This book provides a comprehensive review of the status and trends of marine biodiversity, an impact assessment of human activities and suggests a conceptual framework for relationships between biodiversity and human societies. Further research needs and priorities are identified to sustain the development of integrated scenarios and policies, considering a sustainable development as well as biodiversity conservation. The book is the product of a collective scientific expertise based upon international experts originating from social, biological and ecological sciences.

Note: Description based upon print version of record , AcknowledgementsForeword -- Introduction -- The importance of marine biodiversity -- Key features -- Hierarchical components -- The impacts of human activities on marine biodiversity -- The strategic values of research -- Status and trends -- How many marine species are there? -- Taxonomic records -- Cryptic species -- The DNA barcode -- The drive to identify new species -- The "taxonomic impediment" -- Species under pressure -- Extinct species -- Endangered species -- Ecosystems under pressure: the deep sea -- Spatial patterning of characteristics -- Temporal patterns -- Conceptualising biodiversity -- Conceptual frameworks for relationships between biodiversity and human societies -- Choice of model framework -- Measuring biodiversity -- Biodiversity as a macroscopic descriptor in the European Union Marine Strategy Framework Directive (MSFD) -- Drivers of changes in biodiversity and its uses -- Environmental drivers: a working framework -- Integrated scenarios and policies -- Policies and decision support -- Developing scenarios -- Quantitative methods, models and integrated assessment -- Research needs -- The framework: environmental research -- Naturalistic dimensions -- Human dimensions of research -- Developing modelling: a summarising approach -- Sources -- References -- Databases -- Abbreviations and acronyms -- Group of experts.

Additional Edition: ISBN 9789401785655

Additional Edition: Druckausg. ISBN 978-940-178-565-5

Language: English

Subjects: Biology

Keywords: Meeresökosystem ; Biodiversität

DOI: 10.1007/978-94-017-8566-2

URL: Volltext

URL: Volltext  (lizenzpflichtig)

URL: Cover

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Format: Online-Ressource (XIII, 380 p. 54 illus., 6 illus. in color, digital)

ISBN: 9789400745315

Series Statement: Topics in Current Genetics 23

Content: The post-genomic era has brought new challenges and opportunities in all fields of the biology. In this context, several genome engineering technologies have emerged that will help deciphering genes function by as well as improve gene therapy strategies. Genomic modifications such as knock-in, knock-out, knock-down, sequence replacement or modification can today be routinely performed. However, in front of this large palette of methodologies scientists may experience difficulties to gather useful informations scattered within the literature. This book aims to present the state of this field from basic mechanisms of site-directed modifications to their applications in a wide range of organisms such as bacteria, yeast, plants, insects, mammals. It will discuss the problems encountered when using the random integration strategy and present the recent advances made in targeted genome modification. Technologies based on Zinc Finger nucleases, Meganucleases, TALEN, CRE and FLP recombinase, ?C31 integrase, transposases and resolvases are fully detailed with their strengths and weaknesses. All these informations will help students and experienced researchers to understand and choose the best technology for their own purposes.

Note: Description based upon print version of record , Site-directed Insertion of Transgenes; Prologue; Contents; Contributors; Part I: Site-Directed Integration of Transgenes; Chapter 1: Transgene Site-Specific Integration: Problems and Solutions; 1.1 Introduction; 1.2 Random Integration and Clonal Dominance: Reality or Myth?; 1.2.1 Viral Vectors and Their Integration Profiles; 1.2.2 Non-viral Vectors and Their Integration Profiles; 1.3 Principal Drawbacks of Gene Transfer Integrative Systems and Solutions; 1.3.1 Vector-on-Host Effects: Insertional Mutagenesis; 1.3.2 Host-on-Vector Effects; 1.3.2.1 Innate Immunity , 1.3.2.2 Transgene Regulation and SilencingChromatin Structure; Epigenetic Modulation; Extrinsic Environmental Factors; 1.3.3 Optimizing the Vector Architecture: Solutions to Ensure Safe and Long-Term Expression; 1.3.3.1 Limiting the Innate Immune Response; 1.3.3.2 Limiting Genotoxicity Using Regulatory Components; 1.3.3.3 Ensure Expression by Modifying Vector Architecture; 1.3.3.4 Number of Insertions Acquired per Cell and Hot Spots of Integration Bias; 1.4 Combining Long-Term Expression and Secure Integration by Specific Locus Targeting: Old Solutions to New Perspectives , 1.4.1 Engineering Integrative Enzymes1.4.1.1 Recombinases; 1.4.1.2 Meganucleases; 1.4.1.3 Zinc-Finger Nucleases; 1.4.1.4 Viral Integrases; 1.4.1.5 Transposases; 1.4.1.6 Other Existing Solutions; 1.4.2 What Defines a Good Insertion Site?; 1.4.2.1 Integration into Repetitive Sequences; Integration into Widely Transcribed Repetitive Genes; Integration into Telomeric Repeats; 1.4.2.2 Integration in Specific Loci; HPRT Locus; Locus ROSA26; AAVsite1; 1.4.2.3 Random Integration; 1.5 Conclusion; References , Chapter 2: Designing Non-viral Targeted Integrating Vectors for Genome Engineering in Vertebrates2.1 Introduction: Ideal Integrating Vectors and Safe Insertion Sites; 2.2 Zinc Finger Nucleases; 2.3 TALE Nucleases; 2.4 Meganuclease-Based Vectors; 2.5 Site-Specific Recombinase-Based Integrating Vectors; 2.5.1 Site-Specific Tyrosine Recombinases-Based Vectors; 2.5.2 Site-Specific Serine Recombinase, ΦC31-Based Vectors; 2.6 Design of Random Integrating Vectors Based on DNA Transposons and Associated-Targeting Strategy; 2.6.1 Diversity of DNA-Transposon Based Vectors , 2.6.2 Targeting Strategies of DNA Transposon-Based Systems2.6.2.1 Construction of Chimeric Transposases by Fusion to Zinc Finger DBDs; 2.6.2.2 Construction of Bifunctional Targeting Proteins; 2.7 Conclusion; References; Part II: Integration Based on Homologous Recombination; Chapter 3: Gene Targeting and Homologous Recombination in Saccharomyces cerevisiae; 3.1 Introduction; 3.1.1 History; 3.1.2 Outline; 3.2 Transformation with Linearized Plasmids: Targeted Integration; 3.2.1 Ends-in Integration; 3.2.1.1 General Mechanism; 3.2.1.2 Optimizing "Ends-in" Integration , 3.2.1.3 Tagging a Chromosomal Locus in Vivo by "Ends-in" Integration

Additional Edition: ISBN 9789400745308

Additional Edition: Buchausg. u.d.T. ISBN 978-94-007-4530-8

Language: English

Subjects: Biology

DOI: 10.1007/978-94-007-4531-5

URL: Volltext

URL: Volltext  (lizenzpflichtig)

URL: Cover

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Format: Online-Ressource

ISBN: 9781461412229

Series Statement: Advances in Experimental Medicine and Biology 729

Content: Michael P. Lisanti

Content: Caveolae are 50-100 nm flask-shaped invaginations of the plasma membrane that are primarily composed of cholesterol and sphingolipids. Using modern electron microscopy techniques, caveolae can be observed as omega-shaped invaginations of the plasma membrane, fully-invaginated caveolae, grape-like clusters of interconnected caveolae (caveosome), or as transcellular channels as a consequence of the fusion of individual caveolae. The caveolin gene family consists of three distinct members, namely Cav-1, Cav-2 and Cav-3. Cav-1 and Cav-2 proteins are usually co-expressed and particularly abundant in epithelial, endothelial, and smooth muscle cells as well as adipocytes and fibroblasts. On the other hand, the Cav-3 protein appears to be muscle-specific and is therefore only expressed in smooth, skeletal and cardiac muscles. Caveolin proteins form high molecular weight homo- and/or hetero-oligomers and assume an unusual topology with both their N- and C-terminal domains facing the cytoplasm. Jean-François Jasmin, PhD, obtained his degree at the University of Montreal (Montreal, Canada) in 2004. From 2004 to 2007, he was a Post-Doctoral Fellow at both the Albert Einstein College of Medicine (Bronx, NY; Department of Molecular Pharmacology) and the Thomas Jefferson University (Philadelphia, PA; Department of Cancer Biology). Currently, he is an Assistant Professor (Tenure-Track) in the Department of Stem Cell Biology and Regenerative Medicine at the Thomas Jefferson University (Philadelphia, PA). The current focus of his laboratory is on the role of caveolin proteins in the development of cardiovascular and pulmonary diseases. Philippe G. Frank, PhD, obtained his degree in 1998 at the University of Ottawa (Ottawa, Canada), under mentor Professor Yves L. Marcel, a pioneer in lipoprotein studies. Dr. Frank's doctoral dissertation examined the role and function of apolipoprotein A-I in the reverse cholesterol transport pathway. Also in 1998, he continued his career with a post-doctoral fellowship at the Albert Einstein College of Medicine (Bronx, NY). There, his project focused on the role of caveolin proteins in cancer and atherosclerosis, in addition to lipoprotein and cholesterol metabolism. In 2006, he joined the Kimmel Cancer Center as an Assistant Professor at Thomas Jefferson University in Philadelphia, Pennsylvania, where he focuses on the role of lipoproteins in cancer and vascular diseases. Michael P. Lisanti, MD, PhD, obtained his degrees at Cornell University Medical College (New York, NY) in 1992. From 1992-97, he was a Fellow at the Whitehead Institute at MIT (Cambridge, MA), affiliated with Dr. Harvey Lodish's laboratory. Currently, he is Chairman of the Stem Cell Biology and Regenerative Medicine Department, Leader/ Director of the Program in Molecular Biology and Genetics of Cancer, and Director of the Stem Cell Biology and Regenerative Medicine Center at the Thomas Jefferson University (Philadelphia, PA) as well as Editor-in-Chief of the American Journal of Pathology. The current focus of his laboratory is on the role of caveolin-1 in cancer pathogenesis.

Note: Description based upon print version of record , Title Page; Copyright Page; PREFACE; ABOUT THE EDITORS...; ABOUT THE EDITORS...; ABOUT THE EDITORS...; PARTICIPANTS; Table of Contents; SECTION I IDENTIFICATION AND CELLULAR FUNCTIONS OF CAVEOLAE AND CAVEOLINS; CHAPTER 1 LIPID RAFTS, CAVEOLAE AND GPI-LINKED PROTEINS; INTRODUCTION: LIPID RAFTS; CAVEOLAE; CHOLESTEROL; CAVEOLIN-1 ROLE AND FUNCTION; CAVEOLIN-2 ROLE AND FUNCTION; CAVEOLIN-3 ROLE AND FUNCTION; PTRF; CONCLUSION; REFERENCES; CHAPTER 2 CAVEOLAE AND THE REGULATION OF ENDOCYTOSIS; INTRODUCTION; CAVEOLAE AT THE PLASMA MEMBRANE; INTERNALIZATION OF CAVEOLAE , Are Caveolae Stable and Highly Immobile Invaginations at the Plasma Membrane?Caveolar Budding and Pinching Off from the Plasma Membrane; INTRACELLULAR ROUTE OF CAVEOLAE; REGULATION OF CAVEOLAR TRAFFICKING; CONCLUSION; ACKNOWLEDGEMENTS; REFERENCES; CHAPTER 3 CAVEOLIN-1: Role in Cell Signaling; INTRODUCTION: COMPLEXITY OF CAVEOLIN MEMBRANE DOMAINS; Structure of Cav1; Cav1-Associated Membrane Domains: Rafts, Caveolae and Cav1 Scaffolds; Cav1: Up and Down Regulation in Cancer; SCAFFOLDING DOMAIN AND TYROSINE KINASE RECEPTOR REGULATION; Complexity of Tyrosine Kinase Receptor Regulation by Cav1 , Regulation of EGF Receptor Signaling by Cav1CAV1 REGULATION OF CELL PROLIFERATION, SURVIVAL AND DIFFERENTIATION; Cav1 is an Antiproliferative Factor that Down-Regulates Cyclin D1 Expression; Cav1 Prevents MAP Kinase and PI3 Kinase Signaling Pathways; Cav1 Down-Regulates the -Catenin-TCF/LEF Signaling Pathway; Cell Survival and Drug Resistance to Cell Death; Cav1 as Pro-Apoptotic Factor; Cav1 as a Pro-Survival Factor; Regulation of Cell Phenotype and Differentiation; CAV1 REGULATION OF SIGNALING PATHWAYS IN CELL MIGRATION; Regulation of Cell Migration , Cav1 Participates to Polarity of Endothelial Migrating CellsPhosphorylated Cav1 Regulates Focal Adhesion Signaling and Structure; Central Role of Cav1 in Outside-In and Inside-Out Integrin Signaling: The Cav1-Src-Rho Loop; Cav1 in Mechanotransduction; CONCLUSION: Cav1 OR THE YIN AND THE YANG OF SIGNALING REGULATION; ACKNOWLEDGEMENT; REFERENCES; CHAPTER 4 REGULATION OF eNOS IN CAVEOLAE; INTRODUCTION; eNOS LOCALIZATION IN ENDOTHELIAL CELL CAVEOLAE; CAVEOLIN AND THE REGULATION OF eNOS; HDL ACTIVATION OF eNOS; ESTROGEN MODULATION OF eNOS; CRP MODULATION OF eNOS; CONCLUSION; ACKNOWLEDGEMENTS , REFERENCESSECTION II CAVEOLAE AND CAVEOLINS IN HUMAN DISEASES; CHAPTER 5 RECENT DEVELOPMENTS IN THE INTERACTIONS BETWEEN CAVEOLIN AND PATHOGENS; INTRODUCTION; VIRUSES; BACTERIA; PROTOZOAN PARASITES; Leishmania; Trypanosoma cruzi; Toxoplasma gondii; FUNGI; CONCLUSION; ACKNOWLEDGEMENTS; REFERENCES; CHAPTER 6 CAVEOLIN-1 AND BREAST CANCER :A New Clinical Perspective; INTRODUCTION; CAVEOLIN-1, A POTENT SUPPRESSOR OF GROWTH IN BREAST CANCER CELLS; INSIGHTS FROM THE CAVEOLIN-1 KNOCKOUT MOUSE MODEL; CAVEOLIN-1 MUTATIONS FOUND IN BREAST CANCER PATIENTS , CAVEOLIN-1, A REGULATOR OF ESTROGEN-DEPENDENT SIGNALING AND PROLIFERATION , Lipid rafts, caveolae, and GPI linked proteins / Valerie L. Reeves, Candice M. Thomas, and Eric J. Smart -- Caveolae and the regulation of endocytosis / Anna L. Kiss -- Caveolin : role in cell signaling / Cecile Boscher and Ivan Robert Nabi -- Regulation of ENOS in caveolae / Chieko Mineo and Philip W. Shaul -- Recent developments in the interactions between caveolin and pathogens / Fabiana S. Machado ... [et al.] -- Caveolin and breast cancer : a new clinical perspective / Isabelle Mercier and Michael P. Lisanti -- Caveolin and prostate cancer progression / Michael R. Freeman, Wei Yang, and Dolores Di Vizio -- Caveolins and caveolae, roles in insulin signalling, and diabetes / Peter Strolfors -- Atherosclerosis, caveolae, and caveolin / Stephanos Pavlides ... [et al.] -- Caveolins and heart diseases / Mathivadhani Panneerselvam, Hemal H. Patel, and David M. Roth -- Caveolins and lung function / Nikolaos A. Maniatis ... [et al.].

Additional Edition: ISBN 9781461412212

Additional Edition: Buchausg. u.d.T. Caveolins and caveolae New York, NY : Springer Science + Business Media, LLC, 2012 ISBN 9781461412212

Language: English

Subjects: Biology

DOI: 10.1007/978-1-4614-1222-9

URL: Volltext

URL: Volltext  (lizenzpflichtig)

URL: Cover

UID:

Format: XXVI, 723 S. , Ill., graph. Darst. , 25 cm

Edition: 2. ed., 4. printing

ISBN: 0521730716 , 0521877105 , 9780521730716 , 9780521877107

Note: Previous ed.: 2003 , Formerly CIP , Includes bibliographical references and index. - Previous ed.: 2003 , Hier auch später erschienene, unveränderte Nachdrucke

Language: English

Subjects: Biology

Keywords: DNS ; Sequenzanalyse ; Phylogenetische Systematik ; Proteine ; Sequenzanalyse ; Phylogenetische Systematik