UID:
almafu_9959000052802883
Umfang:
1 online resource (449 p.)
Ausgabe:
1st ed. 1998.
ISBN:
1-280-83601-6
,
9786610836017
,
1-59259-268-6
Serie:
Methods in Molecular Biology, 82
Inhalt:
n Arabidopsis Protocols internationally recognized experts offer a comprehensive collection of significant new methods for gene mapping, cloning, and reverse genetics, as well as updated standard protocols for work in Arabidopsis genetics and molecular biology. These time-tested methods range from techniques for the successful growing of Arabidopsis to strategies for gene cloning. The sections on genetics, transformation, and gene expression analysis will be especially helpful to scientists involved in mutant analysis or in producing and analyzing transgenic plants. In many cases, the protocols can also be applied to other plant species, with minor adjustments. Serving both experienced researchers and beginners in the field, Arabidopsis Protocols will immediately become the standard reference for laboratories working with Arabidopsis, the plant of choice to approach many fundamental questions in plant biology.
Anmerkung:
Description based upon print version of record.
,
Arabidopsis Culture -- Growth of Plants and Preservation of Seeds -- Sterile Techniques in Arabidopsis -- Control of Pests and Diseases of Arabidopsis -- Establishment and Maintenance of Cell Suspension Cultures -- Callus Culture and Regeneration -- Protoplast Isolation, Culture, and Regeneration -- Purification of Subcellular Organelles and Macromolecules -- Preparation of Physiologically Active Chloroplasts from Arabidopsis -- Purification of Mitochondria from Arabidopsis -- Preparation of DNA from Arabidopsis -- High Molecular Weight DNA Extraction from Arabidopsis -- Chloroplast DNA Isolation -- 12 Purification of Mitochondrial DNA from Green Tissues of Arabidopsis -- Preparation of RNA -- Mutagenesis and Genetic Analysis -- 14Seed Mutagenesis of Arabidopsis -- Genetic Analysis -- Cytogenetic Analysis of Arabidopsis -- PCR-Based Identification of T-DNA Insertion Mutants -- Gene Mapping in Arabidopsis -- The Use of Recombinant Inbred Lines (RILs) for Genetic Mapping -- AFLP? Fingerprinting of Arabidopsis -- Building a High-Density Genetic Map Using the AFLP? Technology -- 21. Use of Cleaved Amplified Polymorphic Sequences (CAPS) as Genetic Markers in Arabidopsis thahna -- Mapping Mutations with ARMS -- 23. Mapping Cloned Sequences on YACs -- Transient and Stable Transformation -- Transient Gene Expression in Protoplasts of Arabidopsis thaliana -- Transient Expression of Foreign Genes in Tissues of Arabidopsis thaliana by Bombardment-Mediated Transformation -- Root Transformation by Agrobacterium tumefaciens -- Transformation of Arabidopsis thaliana C24 Leaf Discs by Agrobacterium tumefaciens -- In Planta AgrobacteriumMediated Transformation of Adult Arabidopsis thaliana Plants by Vacuum Infiltration -- PEG-Mediated Protoplast Transformation with Naked DNA -- VI Gene Cloning Strategies -- 30 Cloning Genes of Arabidopsis thaliana by Chromosome Walking -- Chromosome Landing Using an AFLPTWased Strategy -- Transposon Tagging with AC/Ds in Arabidopsis -- 33. Transposon Tagging with the En-l System -- Cloning Genes from T-DNA Tagged Mutants -- Gene Expression Analyses -- In Situ Hybridization -- Whole-Mount In Situ Hybridization in Plants -- Bacterial and Coelenterate Luciferases as Reporter Genes in Plant Cells -- P-Glucuronidase Enzyme Histochemistry on Semithin Sections of Plastic-Embedded Arabidopsis Explants -- In Situ Hybridization to RNA in Whole Arabidopsis Plants -- In Vivo Footprinting in Arabidopsis.
,
English
Weitere Ausg.:
ISBN 0-585-28242-0
Weitere Ausg.:
ISBN 0-89603-391-0
Sprache:
Englisch
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