In:
FEBS Letters, Wiley, Vol. 235, No. 1-2 ( 1988-08), p. 241-246
Abstract:
A new class II restriction endonuclease, Asn I, with a novel sequence specificity was isolated from the Gram‐positive eubacterium Arthrobacter species, strain N‐CM. Asn I recognizes the unambiguously defined palindromic hexanucleotide
consisting of A‐ and T‐residues. The novel enzyme in the presence of Mg 2+ cleaves specifically both strands as indicated by the arrows. The staggered cuts generate 5′‐protruding ends with single‐stranded 5′‐TA‐3′ dinucleotide extensions. The novel enzyme may be a useful tool for cloning experiments by complementation of the few enzymes such as Pst I and Pvu I cutting only once in the Amp r ‐gene of plasmids pBR322 and pBR328.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/0014-5793(88)81271-7
Language:
English
Publisher:
Wiley
Publication Date:
1988
detail.hit.zdb_id:
1460391-3
SSG:
12
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