In:
Molecular and Cellular Biology, Informa UK Limited, Vol. 6, No. 1 ( 1986-01), p. 134-141
Abstract:
The S region of the murine major histocompatibility complex contains two closely related genes: C4, encoding the fourth component of complement, and Slp, encoding sex-limited protein. We cloned these genes from a cosmid library of the B10.W7R strain that does not show androgen regulation of the Slp protein. Restriction site polymorphisms revealed at least four C4-like genes within the Sw7 locus, indicating evolutionary amplification of this region. Transfection of these genes into L cells resulted in expression, processing, and secretion of immunologically correct C4 and Slp proteins. At least two different Slp genes and one C4 gene were capable, after transfection, of expressing C4 and Slp indistinguishable from macrophage-derived protein. A third Slp gene exists within this locus whose recombinant cognate did not express in L cells. Thus, the B10.W7R S region includes one C4 gene and at least three Slp-like genes.
Type of Medium:
Online Resource
ISSN:
0270-7306
,
1098-5549
Language:
English
Publisher:
Informa UK Limited
Publication Date:
1986
detail.hit.zdb_id:
1474919-1
SSG:
12
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