In:
Wound Repair and Regeneration, Wiley, Vol. 19, No. 5 ( 2011-09), p. 597-607
Abstract:
The pathophysiology leading to delayed wound healing is complex and efficient therapeutic approaches for accelerated wound healing currently do not exist. We developed a novel drug‐eluting platform for the potential use in wound dressings. Here, we report on the potential of eluting ascorbic acid‐2‐phosphate ( ASC ‐2 P ), a highly stable variant of ascorbic acid, to induce angiogenesis and to promote collagen synthesis by fibroblasts. The drug‐eluting platform device ( DEPD ) consists of biocompatible polymeric layers comprising polyethylene terephtalate, polyvinyl alcohol ( PVA ), and polyurethane with PVA as the solvent for ASC ‐2 P . The angiogenic potential of ASC ‐2 P was evaluated in the endothelial cell tube formation assay ( TFA ) and in the chorion allantoic membrane ( CAM ) model. Collagen synthesis by ASC ‐2 P ‐stimulated fibroblasts was determined by S irius R ed staining. ASC ‐2 P significantly induced angiogenesis in five independent TFA and CAM assays and induced collagen synthesis in two different fibroblast cell lines. The eluting kinetics of ASC ‐2 P was determined by the ultraviolet N ano D rop method and the functional 2,2′‐Azinobis‐(3‐ethylbenzthiazolin‐6‐sulfonic acid) method. Eluting profiles showed a continuous release in the range of biologically effective concentrations 〉 10 days. This is the first report showing the proangiogenic‐ and collagen‐promoting features of ASC ‐2 P . DEPD loaded with ASC ‐2 P ought to be further evaluated as wound dressings or as supplementary pads for topical treatment of delayed wound healing in preclinical studies.
Type of Medium:
Online Resource
ISSN:
1067-1927
,
1524-475X
DOI:
10.1111/wrr.2011.19.issue-5
DOI:
10.1111/j.1524-475X.2011.00718.x
Language:
English
Publisher:
Wiley
Publication Date:
2011
detail.hit.zdb_id:
2011990-2
Bookmarklink
