Kooperativer Bibliotheksverbund

UID:

Format: 1 online resource (807 p.)

Edition: 1st ed. 2008.

ISBN: 1-59745-376-5

Series Statement: Methods in Molecular Biology, 384

Content: Capillary Electrophoresis: Methods and Protocols presents a selection of current capillary electrophoresis methods used to separate representative types of molecules and particles and in combination with different detection techniques. The volume is intended for beginners in the field and provides overviews of each technique and a starting point for the exploration of the defined literature on the different application topics. As a beginning text, each technique is explained in simple terms, assuming only minimal previous knowledge of the methods. A Notes section accompanies each chapter and addresses problems that may occur and how to overcome them. In Part I, Analyte-Oriented, contributors present methods and protocols based on sample type and size for inorganic and organic small ions, anionic, cationic, and neutral pollutants and pharmaceuticals, sugars, amino acids, nucleotides and DNA, and synthetic copolymers up to microorganisms. In Part II, Methods-Oriented, authors present the more methodological descriptions of particular and new CE techniques including CZE, MEKC/MECC, CEC, affinity CE, coating and online concentration techniques, single-cell analysis, and multidimensional techniques. Comprehensive and timely, Capillary Electrophoresis: Methods and Protocols is ideal for researchers looking for a beginning text on capillary electrophoresis methods.

Note: Bibliographic Level Mode of Issuance: Monograph , Analyte-Oriented -- Determination of Small Ions With Capillary Electrophoresis and Contactless Conductivity Detection -- Metal Analysis With Capillary Zone Electrophoresis -- Measurement of Low-Molecular-Weight Carboxylic Acids in Ambient Air and Vehicle Emission by Capillary Electrophoresis -- Determination of Aliphatic Low-Molecular-Weight and Biogenic Amines by Capillary Zone Electrophoresis -- Capillary Electrophoretic Analysis of Organic Pollutants -- Capillary Electrophoresis With Laser-Induced Fluorescence -- Practical Considerations for the Analysis of Ionic and Neutral Organic Molecules With Capillary Electrophoresis/Mass Spectrometry -- Analysis of the Enantiomers of Chiral Pesticides and Other Pollutants in Environmental Samples by Capillary Electrophoresis -- Capillary Electrophoresis of Tropane Alkaloids and Glycoalkaloids Occurring in Solanaceae Plants -- Capillary Electrophoresis for Pharmaceutical Analysis -- Capillary Electrophoresis of Neutral Carbohydrates -- Capillary Electrophoresis of Sugar Acids -- Use of Capillary Electrophoresis for Polysaccharide Studies and Applications -- Analysis of Oligonucleotides Using Capillary Zone Electrophoresis and Electrospray Mass Spectrometry -- Separation of Dna by Capillary Electrophoresis -- Capillary Electrophoresis of Oxidative Dna Damage -- Capillary Electrophoresis of Gene Mutation -- Biomedical Applications of Amino Acid Detection by Capillary Electrophoresis -- Separation of Peptides by Capillary Electrophoresis -- Analysis of Proteins by Capillary Electrophoresis -- Separation of Synthetic (Co)Polymers by Capillary Electrophoresis Techniques -- Capillary Electrophoresis Separation of Microorganisms -- Methods-Oriented -- A Semi-Empirical Approach for a Rapid Comprehensive Evaluation of the Electrophoretic Behaviors of Small Molecules in Free-Zone Electrophoresis -- The CE Way of Thinking -- Adsorbed Cationic Polymer Coatings for Enhanced Capillary Electrophoresis/Mass Spectrometry of Proteins -- On-Column Ligand/Receptor Derivatization Coupled to Affinity Capillary Electrophoresis -- On-Line Concentration of Environmental Pollutant Samples by Using Capillary Electrophoresis -- Free-Flow Electrophoresis System for Proteomics Applications -- Microemulsion Electrokinetic Chromatography -- Micellar Electrokinetic Chromatography of Aminoglycosides -- Capillary Electrochromatography and On-Line Concentration -- Analysis of Alkaloids in Single Plant Cells by Capillary Electrophoresis -- Multi-Dimensional Capillary Electrophoresis and Chromatography for Proteomic Analysis. , English

Additional Edition: ISBN 1-58829-539-7

Language: English

DOI: 10.1007/978-1-59745-376-9

UID:

Format: XIX, 809 S. : , graph. Darst.

ISBN: 1-58829-539-7 , 978-1-58829-539-2

Series Statement: Methods in molecular biology 384

Language: English

Subjects: Biology , Medicine

Keywords: Biochemische Analyse ; Kapillarelektrophorese ; Aufsatzsammlung ; Aufsatzsammlung

URL: Cover

URL: http://www.loc.gov/catdir/enhancements/fy0825/2006024134-d.html

URL: Inhaltsverzeichnis

UID:

Format: 1 online resource (780 pages)

ISBN: 0-12-814014-3

Note: Front Cover -- Fundamentals and Applications of Fourier Transform mass Spectrometry -- Copyright -- Contents -- Contributors -- Foreword -- Preface -- Acknowledgments -- Section A: Historical chapters -- Chapter 1: Historical developments in Fourier transform ion cyclotron resonance mass spectrometry -- Introduction -- Developments before 1970 -- 1965 -- 1966 -- 1967 -- 1968 -- 1969 -- Developments from 1970 to 1980 -- 1970 -- 1971 -- 1972 -- 1973 -- 1974 -- 1975 -- 1976 -- 1977 -- 1978 -- 1979 -- 1980 -- Developments from 1981 to 1990 -- Developments from 1991 to 2000 -- Developments from 2001 -- Conclusion -- References -- Section B: Fundamental/technology chapters -- Chapter 2: Fundamentals of Orbitrap analyzer -- Principles of operation -- Non-ideal orbital traps and their calibration -- Advances in signal processing -- Fourier transform methods -- Autocorrelation methods -- Maximum likelihood parameter estimators -- Deconvolution method -- Evolution of the Orbitrap platform and selected applications -- Acknowledgments -- References -- Chapter 3: Fundamentals, strengths, and future directions for Fourier transform ion cyclotron resonance mass spectrometry -- FT-ICR fundamentals -- Significant recent developments in FT-ICR -- References -- Chapter 4: Fundamentals and simulations in FT-ICR-MS -- Ion motion in the electromagnetic field -- Ion motion in traps with quadrupolar type field distribution -- The detection of induced signal by cylindrical geometry electrodes -- Harmonics and multiple electrode detection -- The influence of inharmonicity of electrostatic field and inhomogeneity of the magnetic field on ion motion synchronization ... -- Ion traps with dynamic harmonization -- Coalecsence -- Conclusion -- References -- Chapter 5: Advanced fundamentals in Fourier transform mass spectrometry -- ICR and Orbitrap FTMS: A preamble. , Mass spectra processing: From a single to a summed (averaged) mass spectrum -- Representation of mass spectra in full and reduced profile modes -- FTMS resolution performance: Orbitrap and ICR -- Conclusions -- Acknowledgments -- References -- Chapter 6: Data processing and automation in Fourier transform mass spectrometry -- Introduction -- Noise and de-noising in FTMS -- Correct assignment of chemically relevant peaks in FTMS -- Magnitude mode detection in FT-ICR-MS -- Absorption mode detection in FT-ICR-MS -- Non-Fourier transform techniques -- Apodization -- Calibration -- The physics behind the need of additional terms in the calibration equation -- Further developed external calibration equations -- Beat patterns in time-domain FTMS transients [121] -- FT artifacts in FTMS and their implications on data processing -- Batch processing of FTMS mass spectra -- Automation of FTMS instruments -- Acknowledgments -- References -- Chapter 7: Fundamentals of two dimensional Fourier transform mass spectrometry -- Introduction -- Contemporary FT-ICR mass spectrometers and tandem mass spectrometry -- Mass spectrometry in the second dimension -- Interpretation of a 2D mass spectrum -- Noise in 2D-MS -- Resolving power and mass accuracy -- Alternative 2D-MS -- MSn/2D-MS -- Data acquisition and processing -- Data analysis -- Applications of 2D-MS -- Conclusion -- Glossary -- References -- Chapter 8: Trapped ion mobility spectrometry coupled to FT-ICR MS: Fundamentals and applications -- Introduction -- Background -- TIMS analyzer -- TIMS modes of operation -- SA-TIMS -- OSA-TIMS -- G-TIMS (linear, non-linear targeting and nonlinear stepping) -- 2D-TIMS-FT-ICR MS plots and Ko determination -- TIMS-TOF MS vs. TIMS-FT-ICR MS -- Conclusions -- Acknowledgments -- References. , Chapter 9: In situ metabolomics in cancer tissue by high-resolution mass spectrometry imaging -- Introduction -- Common preservation methods for cancer tissues -- MALDI-FT-ICR MSI in metabolomic-based cancer research -- Data processing in high-resolution MALDI MSI -- Discovery of diagnostic markers and tissue-based disease classification by mass spectrometry imaging -- Inter- and intratumoral heterogeneity at metabolite levels -- Therapy response prediction and prognosis -- Conclusion -- References -- Chapter 10: Fourier transform ion cyclotron resonance mass spectrometry and laser: A versatile tool -- Introduction -- Laser-matter and laser-molecule interactions in the solid or in the gas phase -- Laser-matter interaction in the solid phase -- Introduction -- General concepts of laser-matter interaction -- Standing of the laser fluence and laser irradiance -- Simulation of laser-matter interaction and influence of the electrons -- Principles of matrix-assisted laser desorption/ionization -- The laser induced acoustic desorption (LIAD) technique -- Laser-molecule or laser-ion interaction in the gas phase -- Laser gas phase ionization -- Laser gas phase ion dissociation -- Coupling of FT-ICR MS instruments to laser ionization sources -- First instruments fitted with internal ion sources -- Instruments fitted with external ion sources -- Laser-based ionization techniques at atmospheric pressure -- Mass spectrometry imaging (MSI or IMS) -- Some applications of laser ionization coupled to FT-ICR MS -- Inorganic compounds -- Study of inorganic cluster ions -- Study of gas phase thermochemistry of ions and cluster ions in the FT ICR cell -- Organic compounds -- Petroleomics (petroleum and bio-oils) -- Environmental organic contaminants -- Conclusions -- References -- Further reading -- Section C: Applications chapters. , Chapter 11: Metabolomics using Fourier transform mass spectrometry -- Introduction -- The metabolomic workflow -- Sample preparation -- Data acquisition -- Data preprocessing -- Statistical analyses -- Metabolite identification -- Data visualization -- The input of Fourier mass spectrometry to metabolite detection and quantification -- Direct introduction mass spectrometry -- LC/HRMS-based metabolomics -- High-throughput metabolomics -- Towards high-throughput LC/HRMS-MS metabolomics -- Handling and processing of Fourier transform mass spectrometry based metabolomic data -- Automatic peak detection, alignment and integration of features -- Removal of artifactual features -- Correction of analytical drifts and batch to batch variations -- Integrated data processing workflows -- The input of Fourier transform mass spectrometry to metabolome annotation and metabolite identification -- Conclusion and perspectives -- Acknowledgment -- References -- Chapter 12: Mass differences in metabolome analyses of untargeted direct infusion ultra-high resolution MS data -- Introduction to metabolomics -- Primary analysis: Annotation, identification, knowns and unknowns -- Secondary analysis: Pathways and fluxes -- Meaning and application of mass differences in mass spectrometry -- Mass differences in instrumental quality parameters -- Mass differences in UHR-MS data visualization -- Compositional space -- Traditional means of UHR-MS data visualization and interpretation -- Mass difference networks in the visualization and primary analysis of UHR-MS data -- MDiNs in primary data analysis -- MDiN's for dereplication -- MDiN's for third and fourth level identification -- MDiN strategies for second level identification -- MDiN strategies for contextualization of 4th level identification. , Mass difference networks in the visualization and secondary analysis of UHR-MS data -- Classical pathway mapping -- MDiNs in pathway modeling -- Genome scale metabolic models and flux analyzes -- Mass difference networks and genome scale models -- Mass difference enrichment analysis (MDEA) -- Conclusion -- References -- Chapter 13: FT-ICR MS and Orbitrap mass spectrometry approaches in environmental chemistry -- General introduction -- Drinking water and the discovery of new disinfection by-products -- DOM precursors of DBPs -- DBPs in drinking water -- DBPs in brackish and seawater -- DBPs in hydraulic fracturing fluids -- Characterization of organic constituents in wastewater and hydraulic fracturing fluids -- The composition of effluent organic matter (EfOM) -- Hydraulic fracturing organic matter -- Non-targeted approaches in characterizing pollutants -- Contaminants in surface and groundwater -- Contaminants in sediments -- Conclusions and suggestions for future work -- Acknowledgments -- References -- Chapter 14: Identification of biologically active peptides by means of Fourier transform mass spectrometry -- Introduction -- FTMS basics -- FTMS for mass fingerprinting of peptides -- Fourier transform tandem mass spectrometry -- Collisional activation -- Electron-based dissociation -- Photoactivation -- Combining different activation methods -- Conclusion -- References -- Chapter 15: Fourier transform mass spectrometry applied to Forensic Chemistry -- Introduction -- Illicit abused drugs -- Marijuana -- Cocaine -- Hallucinogens -- New psychoactive substances -- Foods and beverages falsification -- Evidence analysis -- Conclusions -- References -- Chapter 16: Fourier transform mass spectrometry applied to petroleomics -- Introduction -- Ionization methods -- Electrospray ionization -- Atmospheric pressure chemical ionization (APCI). , Atmospheric pressure photoionization (APPI).

Additional Edition: ISBN 0-12-814013-5

Language: English

UID:

Format: 1 online resource (780 pages)

ISBN: 0-12-814014-3

Note: Front Cover -- Fundamentals and Applications of Fourier Transform mass Spectrometry -- Copyright -- Contents -- Contributors -- Foreword -- Preface -- Acknowledgments -- Section A: Historical chapters -- Chapter 1: Historical developments in Fourier transform ion cyclotron resonance mass spectrometry -- Introduction -- Developments before 1970 -- 1965 -- 1966 -- 1967 -- 1968 -- 1969 -- Developments from 1970 to 1980 -- 1970 -- 1971 -- 1972 -- 1973 -- 1974 -- 1975 -- 1976 -- 1977 -- 1978 -- 1979 -- 1980 -- Developments from 1981 to 1990 -- Developments from 1991 to 2000 -- Developments from 2001 -- Conclusion -- References -- Section B: Fundamental/technology chapters -- Chapter 2: Fundamentals of Orbitrap analyzer -- Principles of operation -- Non-ideal orbital traps and their calibration -- Advances in signal processing -- Fourier transform methods -- Autocorrelation methods -- Maximum likelihood parameter estimators -- Deconvolution method -- Evolution of the Orbitrap platform and selected applications -- Acknowledgments -- References -- Chapter 3: Fundamentals, strengths, and future directions for Fourier transform ion cyclotron resonance mass spectrometry -- FT-ICR fundamentals -- Significant recent developments in FT-ICR -- References -- Chapter 4: Fundamentals and simulations in FT-ICR-MS -- Ion motion in the electromagnetic field -- Ion motion in traps with quadrupolar type field distribution -- The detection of induced signal by cylindrical geometry electrodes -- Harmonics and multiple electrode detection -- The influence of inharmonicity of electrostatic field and inhomogeneity of the magnetic field on ion motion synchronization ... -- Ion traps with dynamic harmonization -- Coalecsence -- Conclusion -- References -- Chapter 5: Advanced fundamentals in Fourier transform mass spectrometry -- ICR and Orbitrap FTMS: A preamble. , Mass spectra processing: From a single to a summed (averaged) mass spectrum -- Representation of mass spectra in full and reduced profile modes -- FTMS resolution performance: Orbitrap and ICR -- Conclusions -- Acknowledgments -- References -- Chapter 6: Data processing and automation in Fourier transform mass spectrometry -- Introduction -- Noise and de-noising in FTMS -- Correct assignment of chemically relevant peaks in FTMS -- Magnitude mode detection in FT-ICR-MS -- Absorption mode detection in FT-ICR-MS -- Non-Fourier transform techniques -- Apodization -- Calibration -- The physics behind the need of additional terms in the calibration equation -- Further developed external calibration equations -- Beat patterns in time-domain FTMS transients [121] -- FT artifacts in FTMS and their implications on data processing -- Batch processing of FTMS mass spectra -- Automation of FTMS instruments -- Acknowledgments -- References -- Chapter 7: Fundamentals of two dimensional Fourier transform mass spectrometry -- Introduction -- Contemporary FT-ICR mass spectrometers and tandem mass spectrometry -- Mass spectrometry in the second dimension -- Interpretation of a 2D mass spectrum -- Noise in 2D-MS -- Resolving power and mass accuracy -- Alternative 2D-MS -- MSn/2D-MS -- Data acquisition and processing -- Data analysis -- Applications of 2D-MS -- Conclusion -- Glossary -- References -- Chapter 8: Trapped ion mobility spectrometry coupled to FT-ICR MS: Fundamentals and applications -- Introduction -- Background -- TIMS analyzer -- TIMS modes of operation -- SA-TIMS -- OSA-TIMS -- G-TIMS (linear, non-linear targeting and nonlinear stepping) -- 2D-TIMS-FT-ICR MS plots and Ko determination -- TIMS-TOF MS vs. TIMS-FT-ICR MS -- Conclusions -- Acknowledgments -- References. , Chapter 9: In situ metabolomics in cancer tissue by high-resolution mass spectrometry imaging -- Introduction -- Common preservation methods for cancer tissues -- MALDI-FT-ICR MSI in metabolomic-based cancer research -- Data processing in high-resolution MALDI MSI -- Discovery of diagnostic markers and tissue-based disease classification by mass spectrometry imaging -- Inter- and intratumoral heterogeneity at metabolite levels -- Therapy response prediction and prognosis -- Conclusion -- References -- Chapter 10: Fourier transform ion cyclotron resonance mass spectrometry and laser: A versatile tool -- Introduction -- Laser-matter and laser-molecule interactions in the solid or in the gas phase -- Laser-matter interaction in the solid phase -- Introduction -- General concepts of laser-matter interaction -- Standing of the laser fluence and laser irradiance -- Simulation of laser-matter interaction and influence of the electrons -- Principles of matrix-assisted laser desorption/ionization -- The laser induced acoustic desorption (LIAD) technique -- Laser-molecule or laser-ion interaction in the gas phase -- Laser gas phase ionization -- Laser gas phase ion dissociation -- Coupling of FT-ICR MS instruments to laser ionization sources -- First instruments fitted with internal ion sources -- Instruments fitted with external ion sources -- Laser-based ionization techniques at atmospheric pressure -- Mass spectrometry imaging (MSI or IMS) -- Some applications of laser ionization coupled to FT-ICR MS -- Inorganic compounds -- Study of inorganic cluster ions -- Study of gas phase thermochemistry of ions and cluster ions in the FT ICR cell -- Organic compounds -- Petroleomics (petroleum and bio-oils) -- Environmental organic contaminants -- Conclusions -- References -- Further reading -- Section C: Applications chapters. , Chapter 11: Metabolomics using Fourier transform mass spectrometry -- Introduction -- The metabolomic workflow -- Sample preparation -- Data acquisition -- Data preprocessing -- Statistical analyses -- Metabolite identification -- Data visualization -- The input of Fourier mass spectrometry to metabolite detection and quantification -- Direct introduction mass spectrometry -- LC/HRMS-based metabolomics -- High-throughput metabolomics -- Towards high-throughput LC/HRMS-MS metabolomics -- Handling and processing of Fourier transform mass spectrometry based metabolomic data -- Automatic peak detection, alignment and integration of features -- Removal of artifactual features -- Correction of analytical drifts and batch to batch variations -- Integrated data processing workflows -- The input of Fourier transform mass spectrometry to metabolome annotation and metabolite identification -- Conclusion and perspectives -- Acknowledgment -- References -- Chapter 12: Mass differences in metabolome analyses of untargeted direct infusion ultra-high resolution MS data -- Introduction to metabolomics -- Primary analysis: Annotation, identification, knowns and unknowns -- Secondary analysis: Pathways and fluxes -- Meaning and application of mass differences in mass spectrometry -- Mass differences in instrumental quality parameters -- Mass differences in UHR-MS data visualization -- Compositional space -- Traditional means of UHR-MS data visualization and interpretation -- Mass difference networks in the visualization and primary analysis of UHR-MS data -- MDiNs in primary data analysis -- MDiN's for dereplication -- MDiN's for third and fourth level identification -- MDiN strategies for second level identification -- MDiN strategies for contextualization of 4th level identification. , Mass difference networks in the visualization and secondary analysis of UHR-MS data -- Classical pathway mapping -- MDiNs in pathway modeling -- Genome scale metabolic models and flux analyzes -- Mass difference networks and genome scale models -- Mass difference enrichment analysis (MDEA) -- Conclusion -- References -- Chapter 13: FT-ICR MS and Orbitrap mass spectrometry approaches in environmental chemistry -- General introduction -- Drinking water and the discovery of new disinfection by-products -- DOM precursors of DBPs -- DBPs in drinking water -- DBPs in brackish and seawater -- DBPs in hydraulic fracturing fluids -- Characterization of organic constituents in wastewater and hydraulic fracturing fluids -- The composition of effluent organic matter (EfOM) -- Hydraulic fracturing organic matter -- Non-targeted approaches in characterizing pollutants -- Contaminants in surface and groundwater -- Contaminants in sediments -- Conclusions and suggestions for future work -- Acknowledgments -- References -- Chapter 14: Identification of biologically active peptides by means of Fourier transform mass spectrometry -- Introduction -- FTMS basics -- FTMS for mass fingerprinting of peptides -- Fourier transform tandem mass spectrometry -- Collisional activation -- Electron-based dissociation -- Photoactivation -- Combining different activation methods -- Conclusion -- References -- Chapter 15: Fourier transform mass spectrometry applied to Forensic Chemistry -- Introduction -- Illicit abused drugs -- Marijuana -- Cocaine -- Hallucinogens -- New psychoactive substances -- Foods and beverages falsification -- Evidence analysis -- Conclusions -- References -- Chapter 16: Fourier transform mass spectrometry applied to petroleomics -- Introduction -- Ionization methods -- Electrospray ionization -- Atmospheric pressure chemical ionization (APCI). , Atmospheric pressure photoionization (APPI).

Additional Edition: ISBN 0-12-814013-5

Language: English

UID:

Format: 1 online resource (780 pages)

ISBN: 0-12-814014-3

Note: Front Cover -- Fundamentals and Applications of Fourier Transform mass Spectrometry -- Copyright -- Contents -- Contributors -- Foreword -- Preface -- Acknowledgments -- Section A: Historical chapters -- Chapter 1: Historical developments in Fourier transform ion cyclotron resonance mass spectrometry -- Introduction -- Developments before 1970 -- 1965 -- 1966 -- 1967 -- 1968 -- 1969 -- Developments from 1970 to 1980 -- 1970 -- 1971 -- 1972 -- 1973 -- 1974 -- 1975 -- 1976 -- 1977 -- 1978 -- 1979 -- 1980 -- Developments from 1981 to 1990 -- Developments from 1991 to 2000 -- Developments from 2001 -- Conclusion -- References -- Section B: Fundamental/technology chapters -- Chapter 2: Fundamentals of Orbitrap analyzer -- Principles of operation -- Non-ideal orbital traps and their calibration -- Advances in signal processing -- Fourier transform methods -- Autocorrelation methods -- Maximum likelihood parameter estimators -- Deconvolution method -- Evolution of the Orbitrap platform and selected applications -- Acknowledgments -- References -- Chapter 3: Fundamentals, strengths, and future directions for Fourier transform ion cyclotron resonance mass spectrometry -- FT-ICR fundamentals -- Significant recent developments in FT-ICR -- References -- Chapter 4: Fundamentals and simulations in FT-ICR-MS -- Ion motion in the electromagnetic field -- Ion motion in traps with quadrupolar type field distribution -- The detection of induced signal by cylindrical geometry electrodes -- Harmonics and multiple electrode detection -- The influence of inharmonicity of electrostatic field and inhomogeneity of the magnetic field on ion motion synchronization ... -- Ion traps with dynamic harmonization -- Coalecsence -- Conclusion -- References -- Chapter 5: Advanced fundamentals in Fourier transform mass spectrometry -- ICR and Orbitrap FTMS: A preamble. , Mass spectra processing: From a single to a summed (averaged) mass spectrum -- Representation of mass spectra in full and reduced profile modes -- FTMS resolution performance: Orbitrap and ICR -- Conclusions -- Acknowledgments -- References -- Chapter 6: Data processing and automation in Fourier transform mass spectrometry -- Introduction -- Noise and de-noising in FTMS -- Correct assignment of chemically relevant peaks in FTMS -- Magnitude mode detection in FT-ICR-MS -- Absorption mode detection in FT-ICR-MS -- Non-Fourier transform techniques -- Apodization -- Calibration -- The physics behind the need of additional terms in the calibration equation -- Further developed external calibration equations -- Beat patterns in time-domain FTMS transients [121] -- FT artifacts in FTMS and their implications on data processing -- Batch processing of FTMS mass spectra -- Automation of FTMS instruments -- Acknowledgments -- References -- Chapter 7: Fundamentals of two dimensional Fourier transform mass spectrometry -- Introduction -- Contemporary FT-ICR mass spectrometers and tandem mass spectrometry -- Mass spectrometry in the second dimension -- Interpretation of a 2D mass spectrum -- Noise in 2D-MS -- Resolving power and mass accuracy -- Alternative 2D-MS -- MSn/2D-MS -- Data acquisition and processing -- Data analysis -- Applications of 2D-MS -- Conclusion -- Glossary -- References -- Chapter 8: Trapped ion mobility spectrometry coupled to FT-ICR MS: Fundamentals and applications -- Introduction -- Background -- TIMS analyzer -- TIMS modes of operation -- SA-TIMS -- OSA-TIMS -- G-TIMS (linear, non-linear targeting and nonlinear stepping) -- 2D-TIMS-FT-ICR MS plots and Ko determination -- TIMS-TOF MS vs. TIMS-FT-ICR MS -- Conclusions -- Acknowledgments -- References. , Chapter 9: In situ metabolomics in cancer tissue by high-resolution mass spectrometry imaging -- Introduction -- Common preservation methods for cancer tissues -- MALDI-FT-ICR MSI in metabolomic-based cancer research -- Data processing in high-resolution MALDI MSI -- Discovery of diagnostic markers and tissue-based disease classification by mass spectrometry imaging -- Inter- and intratumoral heterogeneity at metabolite levels -- Therapy response prediction and prognosis -- Conclusion -- References -- Chapter 10: Fourier transform ion cyclotron resonance mass spectrometry and laser: A versatile tool -- Introduction -- Laser-matter and laser-molecule interactions in the solid or in the gas phase -- Laser-matter interaction in the solid phase -- Introduction -- General concepts of laser-matter interaction -- Standing of the laser fluence and laser irradiance -- Simulation of laser-matter interaction and influence of the electrons -- Principles of matrix-assisted laser desorption/ionization -- The laser induced acoustic desorption (LIAD) technique -- Laser-molecule or laser-ion interaction in the gas phase -- Laser gas phase ionization -- Laser gas phase ion dissociation -- Coupling of FT-ICR MS instruments to laser ionization sources -- First instruments fitted with internal ion sources -- Instruments fitted with external ion sources -- Laser-based ionization techniques at atmospheric pressure -- Mass spectrometry imaging (MSI or IMS) -- Some applications of laser ionization coupled to FT-ICR MS -- Inorganic compounds -- Study of inorganic cluster ions -- Study of gas phase thermochemistry of ions and cluster ions in the FT ICR cell -- Organic compounds -- Petroleomics (petroleum and bio-oils) -- Environmental organic contaminants -- Conclusions -- References -- Further reading -- Section C: Applications chapters. , Chapter 11: Metabolomics using Fourier transform mass spectrometry -- Introduction -- The metabolomic workflow -- Sample preparation -- Data acquisition -- Data preprocessing -- Statistical analyses -- Metabolite identification -- Data visualization -- The input of Fourier mass spectrometry to metabolite detection and quantification -- Direct introduction mass spectrometry -- LC/HRMS-based metabolomics -- High-throughput metabolomics -- Towards high-throughput LC/HRMS-MS metabolomics -- Handling and processing of Fourier transform mass spectrometry based metabolomic data -- Automatic peak detection, alignment and integration of features -- Removal of artifactual features -- Correction of analytical drifts and batch to batch variations -- Integrated data processing workflows -- The input of Fourier transform mass spectrometry to metabolome annotation and metabolite identification -- Conclusion and perspectives -- Acknowledgment -- References -- Chapter 12: Mass differences in metabolome analyses of untargeted direct infusion ultra-high resolution MS data -- Introduction to metabolomics -- Primary analysis: Annotation, identification, knowns and unknowns -- Secondary analysis: Pathways and fluxes -- Meaning and application of mass differences in mass spectrometry -- Mass differences in instrumental quality parameters -- Mass differences in UHR-MS data visualization -- Compositional space -- Traditional means of UHR-MS data visualization and interpretation -- Mass difference networks in the visualization and primary analysis of UHR-MS data -- MDiNs in primary data analysis -- MDiN's for dereplication -- MDiN's for third and fourth level identification -- MDiN strategies for second level identification -- MDiN strategies for contextualization of 4th level identification. , Mass difference networks in the visualization and secondary analysis of UHR-MS data -- Classical pathway mapping -- MDiNs in pathway modeling -- Genome scale metabolic models and flux analyzes -- Mass difference networks and genome scale models -- Mass difference enrichment analysis (MDEA) -- Conclusion -- References -- Chapter 13: FT-ICR MS and Orbitrap mass spectrometry approaches in environmental chemistry -- General introduction -- Drinking water and the discovery of new disinfection by-products -- DOM precursors of DBPs -- DBPs in drinking water -- DBPs in brackish and seawater -- DBPs in hydraulic fracturing fluids -- Characterization of organic constituents in wastewater and hydraulic fracturing fluids -- The composition of effluent organic matter (EfOM) -- Hydraulic fracturing organic matter -- Non-targeted approaches in characterizing pollutants -- Contaminants in surface and groundwater -- Contaminants in sediments -- Conclusions and suggestions for future work -- Acknowledgments -- References -- Chapter 14: Identification of biologically active peptides by means of Fourier transform mass spectrometry -- Introduction -- FTMS basics -- FTMS for mass fingerprinting of peptides -- Fourier transform tandem mass spectrometry -- Collisional activation -- Electron-based dissociation -- Photoactivation -- Combining different activation methods -- Conclusion -- References -- Chapter 15: Fourier transform mass spectrometry applied to Forensic Chemistry -- Introduction -- Illicit abused drugs -- Marijuana -- Cocaine -- Hallucinogens -- New psychoactive substances -- Foods and beverages falsification -- Evidence analysis -- Conclusions -- References -- Chapter 16: Fourier transform mass spectrometry applied to petroleomics -- Introduction -- Ionization methods -- Electrospray ionization -- Atmospheric pressure chemical ionization (APCI). , Atmospheric pressure photoionization (APPI).

Additional Edition: ISBN 0-12-814013-5

Language: English

UID:

In: Aquatic sciences. - 66(2004)2, S. 195-210

UID:

In: Proceedings of the National Academy of Sciences of the United States of America. - 115(2018)11, S. 2670-2675

UID:

ISSN: 1879-1026

In: Science of the Total Environment. - 707(2020),136087

UID:

Format: xxiii, 754 Seiten : , Illustrationen, Diagramme (teilweise farbig).

ISBN: 978-0-12-814013-0 , 0-12-814013-5

Language: English

Subjects: Chemistry/Pharmacy , Physics

URL: http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031565116&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA

UID:

Format: 1 online resource (XI, 527 p. 156 illus., 28 illus. in color.)

Edition: 2nd ed. 2016.

ISBN: 1-4939-6403-8

Series Statement: Methods in Molecular Biology, 1483

Uniform Title: Capillary electrophoresis (Schmitt-Kopplin)

Content: This new edition presents principle methods in capillary electrophoresis (CE) separation involving CZE, MEKC, MECC, NACE, and corresponding hyphenated techniques to organic mass spectrometry and ICP-MS. Recent developments in the techniques of single cell analysis, as well as derivation, enantioseparation or the use of ionic liquids, and the use of CZE for the separation of living cells are also highlighted. This book discusses various application methods for the analysis of small ions, organic acids, amino acids, and (poly)saccharides to peptides that are shown with pollutants and biomarkers in food and health. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and thorough, Capillary Electrophoresis: Methods and Protocols, Second Edition covers a wide field of interests and will be especially great for beginners and students because of its combined focus on mini-reviews and application notes that will help them quickly get an overview of the field.

Note: The CE-Way of Thinking: “All is Relative!” -- A Semi-Empirical Approach for a Rapid Comprehensive Evaluation of the Electrophoretic Behaviors of Small Molecules in Free Zone Electrophoresis -- Derivatization in Capillary Electrophoresis -- Statically Adsorbed Coatings for High Separation Efficiency and Resolution in CE-MS Peptide Analysis-Strategies and Implementation -- Micellar Electrokinetic Chromatography of Aminoglycosides -- Microemulsion Electrokinetic Chromatography -- Non Aqueous Capillary Electrophoresis Mass Spectrometry -- Ionic Liquids in Capillary Electrophoresis -- CZE-CZE ESI-MS Coupling with a Fully Isolated Mechanical Valve -- Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry -- Use of CE to Analyze Solutes in Pico- and Nano-Litre Samples from Plant Cells and Rhizosphere -- Analysis of Small Ions with Capillary Electrophoresis -- Metal Ions Analysis with Capillary Zone Electrophoresis -- Bioanalytical Application of Amino Acid Detection by Capillary Electrophoresis -- Enantiomer Separations by Capillary Electrophoresis -- Capillary Electrophoresis of Mono- and Oligosaccharides -- Use of Capillary Electrophoresis for Polysaccharide Studies and Applications -- Separation of Peptides by Capillary Electrophoresis -- Microbial Analysis of Escherichia coli ATCC, Lactobacteria and Saccharomyces cerevisiae Using Capillary Electrophoresis Approach -- Capillary Electrophoretic Analysis of Classical Organic Pollutants -- Capillary Electrophoresis in Metabolomics -- Capillary Electrophoresis in Food and Foodomics -- Capillary Electrophoresis in Wine Science.

Additional Edition: ISBN 1-4939-6401-1

Language: English

Keywords: Laboratory Manuals.

DOI: 10.1007/978-1-4939-6403-1