UID:
edoccha_9958128901802883
Umfang:
1 online resource (719 p.)
ISBN:
0-08-088422-9
Serie:
Methods in enzymology ; volume 343
Inhalt:
G Protein Pathways is the first of three volumes examining the nature of heterotrimeric G proteins. The text takes an integrated approach to studying common experimental questions at many different levels related to G proteins. Methods related to G proteins using molecular modeling, systems biology, protein engineering, protein biochemistry, cell biology, and physiology are all accessible in the same volume.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each
Anmerkung:
Description based upon print version of record.
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Front Cover; G Protein Pathways; Copyright Page; Table of Contents; Contributors to Volume 343; Preface; Volume in Series; Section I: G Protein-Coupled Receptors; A. Theoretical Evaluation of Receptor Function; B. Design and Use of Receptor Ligands; C. Structural Characterization of Receptor Proteins; D. Design and Use of Engineered Receptor Proteins; E. Molecular Modeling Studies of Receptor Structure and Function; F. Analysis of Receptor Protein Coupling; G. Characterization of Receptor Heterogeneity; H. The Study of Receptor Trafficking
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Chapter 1. Considerations in the Evaluation of Inverse Agonism and Protean Agonism at GProtein-Coupled ReceptorsChapter 2. Theoretical Implications of Receptor Coupling to Multiple G Proteins Based on Analysis of Three-State Model; Chapter 3. Use of Retinal Analogues for the Study of Visual Pigment Function; Chapter 4. Design and Synthesis of Peptide Antagonists and Inverse Agonists for G Protein-Coupled Receptors; Chapter 5. Design of Peptide Agonists; Chapter 6. Design of Nonpeptides from Peptide Ligands for Peptide Receptors
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Chapter 7. Strategies for Mapping the Binding Site of the Serotonin 5-HT2A ReceptorChapter 8. Use of the Substituted Cysteine Accessibility Method to Study the Structure and Function of G Protein-Coupled Receptors; Chapter 9. Mass Spectrometric Analysis of GProtein-Coupled Receptors; Chapter 10. Probing the Higher Order Sructure of G Protein- Coupled Receptors Using Tethered Cleavage Methods; Chapter 11. Use of Fluorescence Spectroscopy to Study Conformational Changes in the β2-Adrenoceptor; Chapter 12. Crystallization of Membrane Proteins in Cubo
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Chapter 13. N-Linked Carbohydrates on G Protein-Coupled Receptors: Mapping Sites of Attachment and Determining Functional RolesChapter 14. Magic Angle Spinning Nuclear Magnetic Resonance of Isotopically Labeled Rhodopsin; Chapter 15. Use of Nuclear Magnetic Resonance to Study the Three-Dimensional Structure of Rhodopsin; Chapter 16. Tools for Dissecting Signaling Pathways in Vivo: Receptors Activated Solely by Synthetic Ligands; Chapter 17. Analysis of Structure-Function from Expression of G Protein-Coupled Receptor Fragments
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Chapter 18. Construction and Analysis of Function of G Protein-Coupled Receptor-G Protein Fusion ProteinsChapter 19. Synthetic Gene Technology: Applications to Ancestral Gene Reconstruction and Structure Function Studies of Receptors; Chapter 20. Considerations in the Design and Use of Chimeric G Protein-Coupled Receptors ; Chapter 21. Strategies for Modeling the Interactions of Transmembrane Helices of G Protein-Coupled Receptors by Geometric Complementarity Using the GRAMM Computer Algorithm
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Chapter 22. Three-Dimensional Representations of G Protein- Coupled Receptor Structures and Mechanisms
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English
Weitere Ausg.:
ISBN 1-322-20131-5
Weitere Ausg.:
ISBN 0-12-182244-3
Sprache:
Englisch
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