In:
Regenerative Medicine, Future Medicine Ltd, Vol. 8, No. 5 ( 2013-09), p. 569-581
Abstract:
Aim: To enumerate and characterize mesenchymal stem cells (MSCs) and endothelial cells (ECs) in umbilical cord (UC) tissue digests. Materials & methods: Cultured UC cells were characterized phenotypically, and functionally by using 48-gene arrays. Native MSCs and ECs were enumerated using flow cytometry. Results: Compared with bone marrow (BM) MSCs, UC MSCs displayed significantly lower (range 4–240-fold) basal levels of bone-related transcripts, but their phenotypes were similar (CD73 + , CD105 + , CD90 + , CD45 - and CD31 - ). UC MSCs responded well to osteogenic induction, but day 21 postinduction levels remained below those achieved by BM MSCs. The total yield of native UC MSCs (CD90 + , CD45 - and CD235α - ) and ECs (CD31 + , CD45 - and CD235α - ) exceeded 150 and 15 million cells/donation, respectively. Both UC MSCs and ECs expressed CD146. Conclusion: While BM MSCs are more predisposed to osteogenesis, UC tissue harbors large numbers of MSCs and ECs; such minimally manipulated ‘off-the-shelf’ cellular mixtures can be used for regenerating bone in patients with compromised vascular supply.
Type of Medium:
Online Resource
ISSN:
1746-0751
,
1746-076X
Language:
English
Publisher:
Future Medicine Ltd
Publication Date:
2013
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