Format:
Online-Ressource
ISSN:
1460-2075
Content:
Su (var) 3–9 is a dominant modifier of heterochromatin‐induced gene silencing. Like its mammalian and Schizosaccharomyces pombe homologues, Su (var) 3–9 encodes a histone methyltransferase (HMTase), which selectively methylates histone H3 at lysine 9 (H3‐K9). In Su (var) 3–9 null mutants, H3‐K9 methylation at chromocentre heterochromatin is strongly reduced, indicating that SU (VAR) 3–9 is the major heterochromatin‐specific HMTase in Drosophila. SU (VAR) 3–9 interacts with the heterochromatin‐associated HP1 protein and with another silencing factor, SU (VAR) 3–7. Notably, SU (VAR) 3–9–HP1 interaction is interdependent and governs distinct localization patterns of both proteins. In Su (var) 3–9 null mutants, concentration of HP1 at the chromocentre is nearly lost without affecting HP1 accumulation at the fourth chromosome. By contrast, in HP1 null mutants SU (VAR) 3–9 is no longer restricted at heterochromatin but broadly dispersed across the chromosomes. Despite this interdependence, Su (var) 3–9 dominates the PEV modifier effects of HP1 and Su (var) 3–7 and is also epistatic to the Y chromosome effect on PEV. Finally, the human SUV39H1 gene is able to partially rescue Su (var) 3–9 silencing defects. Together, these data indicate a central role for the SU (VAR) 3–9 HMTase in heterochromatin‐induced gene silencing in Drosophila.
In:
volume:21
In:
number:5
In:
year:2002
In:
pages:1121-1131
In:
extent:11
In:
European Molecular Biology Organization, The EMBO journal, Heidelberg : EMBO Press, 1982-, 21, Heft 5 (2002), 1121-1131 (gesamt 11), 1460-2075
Language:
English
DOI:
10.1093/emboj/21.5.1121
URN:
urn:nbn:de:101:1-2023110203302095400209
URL:
https://doi.org/10.1093/emboj/21.5.1121
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2023110203302095400209
URL:
https://d-nb.info/1308260016/34
URL:
https://doi.org/10.1093/emboj/21.5.1121
Bookmarklink