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  • 1
    Online Resource
    Online Resource
    Salt and Metal Tolerance Involves Formation of Guttation Droplets in Species of the Aspergillus versicolor Complex
    MDPI AG ; 2022
    In:  Genes Vol. 13, No. 9 ( 2022-09-11), p. 1631-
    Details
    In: Genes, MDPI AG, Vol. 13, No. 9 ( 2022-09-11), p. 1631-
    Abstract: Three strains of the Aspergillus versicolor complex were isolated from a salty marsh at a former uranium mining site in Thuringia, Germany. The strains from a metal-rich environment were not only highly salt tolerant (up to 20% NaCl), but at the same time could sustain elevated Cs and Sr (both up to 100 mM) concentrations as well as other (heavy) metals present in the environment. During growth experiments when screening for differential cell morphology, the occurrence of guttation droplets was observed, specifically when elevated Sr concentrations of 25 mM were present in the media. To analyze the potential of metal tolerance being promoted by these excretions, proteomics and metabolomics of guttation droplets were performed. Indeed, proteins involved in up-regulated metabolic activities as well as in stress responses were identified. The metabolome verified the presence of amino sugars, glucose homeostasis-regulating substances, abscisic acid and bioactive alkaloids, flavones and quinones.
    Type of Medium: Online Resource
    ISSN: 2073-4425
    URL: Article
    DOI: 10.3390/genes13091631
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2527218-4
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  • 2
    Online Resource
    Online Resource
    Biomineralization by Extremely Halophilic and Metal-Tolerant Community Members from a Sulfate-Dominated Metal-Rich Environment
    MDPI AG ; 2021
    In:  Microorganisms Vol. 10, No. 1 ( 2021-12-31), p. 79-
    Details
    In: Microorganisms, MDPI AG, Vol. 10, No. 1 ( 2021-12-31), p. 79-
    Abstract: The adaptation to adverse environmental conditions can lead to adapted microbial communities that may be screened for mechanisms involved in halophily and, in this case, metal tolerance. At a former uranium mining and milling site in Seelingstädt, Germany, microbial communities from surface waters and sediment soils were screened for isolates surviving high salt and metal concentrations. The high salt contents consisted mainly of chloride and sulfate, both in soil and riverbed sediment samples, accompanied by high metal loads with presence of cesium and strontium. The community structure was dominated by Chloroflexi, Proteobacteria and Acidobacteriota, while only at the highest contaminations did Firmicutes and Desulfobacterota reach appreciable percentages in the DNA-based community analysis. The extreme conditions providing high stress were mirrored by low numbers of cultivable strains. Thirty-four extremely halotolerant bacteria (23 Bacillus sp. and another 4 Bacillales, 5 Actinobacteria, and 1 Gamma-Proteobacterium) surviving 25 to 100 mM SrCl2, CsCl, and Cs2SO4 were further analyzed. Mineral formation of strontium- or cesium-struvite could be observed, reducing bioavailability and thereby constituting the dominant metal and salt resistance strategy in this environment.
    Type of Medium: Online Resource
    ISSN: 2076-2607
    URL: Article
    DOI: 10.3390/microorganisms10010079
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2720891-6
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  • 3
    Online Resource
    Online Resource
    Biofilm formation in Gram‐positives as an answer to combined salt and metal stress
    Wiley ; 2023
    In:  Journal of Basic Microbiology Vol. 63, No. 8 ( 2023-08), p. 888-896
    Details
    In: Journal of Basic Microbiology, Wiley, Vol. 63, No. 8 ( 2023-08), p. 888-896
    Abstract: Biofilm formation can lead to tolerance against stressors like antibiotics, toxic metals, salts, and other environmental contaminants. Halo‐ and metal‐tolerant bacilli and actinomycete strains isolated from a former uranium mining and milling site in Germany were shown to form biofilm in response to salt and metal treatment; specifically, Cs and Sr exposition led to biofilm formation. Since the strains were obtained from soil samples, a more structured environment was tested using expanded clay to provide porous structures resembling the natural environment. There, accumulation of Cs could be shown for Bacillus sp. SB53B, and high Sr accumulation ranging from 75% to 90% was seen with all isolates tested. We could, therefore, show that biofilms in a structured environment like soil will contribute to the water purification obtained by the passage of water through the critical zone of soil, providing an ecosystem benefit that can hardly be overestimated.
    Type of Medium: Online Resource
    ISSN: 0233-111X , 1521-4028
    URL: Issue
    URL: Article
    DOI: 10.1002/jobm.v63.8
    DOI: 10.1002/jobm.202300149
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 1480967-9
    detail.hit.zdb_id: 632513-0
    detail.hit.zdb_id: 203025-1
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    Microbiomes in an acidic rock–water cave system
    Oxford University Press (OUP) ; 2019
    In:  FEMS Microbiology Letters Vol. 366, No. 13 ( 2019-07-01)
    Details
    In: FEMS Microbiology Letters, Oxford University Press (OUP), Vol. 366, No. 13 ( 2019-07-01)
    Abstract: Belowground ecosystems are accessible by mining, where a specific microbial community can be discovered. The biodiversity of a former alum mine rich in carbon, but with a low pH of 2.6–3.7, was evaluated by DNA- and cultivation-dependent methods using samples of the black slate rock material, secondary mineralization phases and seepage water. Pyrite oxidation within the low-grade metamorphic Silurian black slate established high concentrations of Fe and $\rm{SO}_4^{2-}$ forming the extreme conditions visible with acidophilic and Fe-oxidizing microorganisms. In addition, an unexpected predominance of fungi in this C-rich and acidic cave ecosystem, including high numbers of Mucoromycota and Mortierellomycota, was detected. Therefore, fungal cultures were obtained, mainly from the secondary mineral phases that are iron phosphates. Hence, the fungi might well have been involved in phosphate mobilization there. The rock material itself is rich in organic carbon that can be used by oxidase activity. The cultivation setup mimicked the cave conditions (low temperature, low pH, oxic conditions), with one oligotrophic and one medium rich in nutrients that allowed for isolation of different fungal (and eutrophic bacterial) groups. The acidic conditions prevented the occurrence of many basidiomycetes, while the isolated fungi could survive these adverse conditions.
    Type of Medium: Online Resource
    ISSN: 1574-6968
    URL: Article
    DOI: 10.1093/femsle/fnz167
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2019
    detail.hit.zdb_id: 1501716-3
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