In:
PLOS Genetics, Public Library of Science (PLoS), Vol. 20, No. 3 ( 2024-3-11), p. e1011059-
Abstract:
RpoS is an alternative sigma factor needed for the induction of the general stress response in many gammaproteobacteria. Tight regulation of RpoS levels and activity is required for bacterial growth and survival under stress. In Escherichia coli , various stresses lead to higher levels of RpoS due to increased translation and decreased degradation. During non-stress conditions, RpoS is unstable, because the adaptor protein RssB delivers RpoS to the ClpXP protease. RpoS degradation is prevented during stress by the sequestration of RssB by anti-adaptors, each of which is induced in response to specific stresses. Here, we examined how the stabilization of RpoS is reversed during recovery of the cell from stress. We found that RpoS degradation quickly resumes after recovery from phosphate starvation, carbon starvation, and when transitioning from stationary phase back to exponential phase. This process is in part mediated by the anti-adaptor IraP, known to promote RpoS stabilization during phosphate starvation via the sequestration of adaptor RssB. The rapid recovery from phosphate starvation is dependent upon a feedback loop in which RpoS transcription of rssB , encoding the adaptor protein, plays a critical role. Crl, an activator of RpoS that specifically binds to and stabilizes the complex between the RNA polymerase and RpoS, is also required for the feedback loop to function efficiently, highlighting a critical role for Crl in restoring RpoS basal levels.
Type of Medium:
Online Resource
ISSN:
1553-7404
DOI:
10.1371/journal.pgen.1011059
DOI:
10.1371/journal.pgen.1011059.g001
DOI:
10.1371/journal.pgen.1011059.g002
DOI:
10.1371/journal.pgen.1011059.g003
DOI:
10.1371/journal.pgen.1011059.g004
DOI:
10.1371/journal.pgen.1011059.g005
DOI:
10.1371/journal.pgen.1011059.g006
DOI:
10.1371/journal.pgen.1011059.g007
DOI:
10.1371/journal.pgen.1011059.g008
DOI:
10.1371/journal.pgen.1011059.g009
DOI:
10.1371/journal.pgen.1011059.g010
DOI:
10.1371/journal.pgen.1011059.s001
DOI:
10.1371/journal.pgen.1011059.s002
DOI:
10.1371/journal.pgen.1011059.s003
DOI:
10.1371/journal.pgen.1011059.s004
DOI:
10.1371/journal.pgen.1011059.s005
DOI:
10.1371/journal.pgen.1011059.s006
DOI:
10.1371/journal.pgen.1011059.s007
DOI:
10.1371/journal.pgen.1011059.s008
DOI:
10.1371/journal.pgen.1011059.s009
DOI:
10.1371/journal.pgen.1011059.s010
DOI:
10.1371/journal.pgen.1011059.s011
DOI:
10.1371/journal.pgen.1011059.s012
DOI:
10.1371/journal.pgen.1011059.s013
DOI:
10.1371/journal.pgen.1011059.s014
DOI:
10.1371/journal.pgen.1011059.s015
DOI:
10.1371/journal.pgen.1011059.r001
DOI:
10.1371/journal.pgen.1011059.r002
DOI:
10.1371/journal.pgen.1011059.r003
DOI:
10.1371/journal.pgen.1011059.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2024
detail.hit.zdb_id:
2186725-2
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