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  • American Society for Microbiology  (64)
  • 1
    Online Resource
    Online Resource
    Type-C RNA Virus Gene Expression in Human Tissue
    American Society for Microbiology ; 1974
    In:  Journal of Virology Vol. 14, No. 6 ( 1974-12), p. 1584-1596
    Details
    In: Journal of Virology, American Society for Microbiology, Vol. 14, No. 6 ( 1974-12), p. 1584-1596
    Abstract: Partially purified fractions of human tissues have been analyzed by competition radioimmunoassay for the presence of two of the principle structural components of type-C RNA viruses, the major core protein (p27 to p30) and the major envelope glycopeptides (gp69/71). Screening of tissues was carried out by use of a heterologous assay system of 125 I-labeled Rauscher murine virus p30 antigen and anti-RD 114 virus serum which was found to detect a class of interspecies determinants common to murine, feline, and primate viruses. A competitor with the same apparent affinity for antibody binding as that of purified viral core proteins was found in relatively high concentration in tissues from patients with systemic lupus erythematosus, in some neoplastic tissues, and also in normal human tissues. This competitor from a lupus spleen chromatographed on phosphocellulose and showed size fractionation during gel filtration similar to known p27 to p30 viral proteins. An immunologically reactive protein was also demonstrated by immunodiffusion and by immunoprecipitation of 125 I-labeled human protein with anti-RD 114 p28 serum. Analysis of these human competitor proteins with homologous assay systems of viral core proteins and corresponding antisera showed that all, including the normal tissue extracts, appear similar to core proteins of known viruses, especially the RD 114 and woolly monkey species. A hypothesis suggested by these data is that many, if not all, humans harbor at least part of the genome of one or more type-C viruses, the properties of which are similar to those of viruses from other mammalian species, particularly primates.
    Type of Medium: Online Resource
    ISSN: 0022-538X , 1098-5514
    URL: Article
    DOI: 10.1128/jvi.14.6.1584-1596.1974
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1974
    detail.hit.zdb_id: 1495529-5
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    Wissenschaftspark Albert Einstein
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  • 2
    Online Resource
    Online Resource
    Inhibition of lymphoproliferation by a synthetic peptide with sequence identity to gp41 of human immunodeficiency virus type 1
    American Society for Microbiology ; 1989
    In:  Journal of Virology Vol. 63, No. 8 ( 1989-08), p. 3257-3260
    Details
    In: Journal of Virology, American Society for Microbiology, Vol. 63, No. 8 ( 1989-08), p. 3257-3260
    Abstract: Peptides were synthesized that contained sequences from two regions (env amino acids [aa] 581 to 597 and 655 to 671) of the transmembrane protein gp41 and one region of the external envelope glycoprotein gp120 (aa 457 to 464) of human immunodeficiency virus type 1. Selection of these sequences was based on their homology to the highly conserved and immunosuppressive sequence contained within the transmembrane proteins p15E and gp21 of animal and human retroviruses, respectively. Peptide aa581-597 was found to specifically inhibit human and murine lymphoproliferation, whereas peptides aa655-671 and aa457-464 had no activity. These results suggest a mechanism by which human immunodeficiency virus type 1 gp41 exerts a direct immunosuppressive effect in vivo, analogous to that postulated for p15E and gp21, which could contribute to the immune dysfunction observed in patients suffering from acquired immunodeficiency syndrome. It is of particular interest that the sequence aa 584 to 609, shown to contain B- and T-helper-cell epitopes, overlaps with the sequence aa 581 to 597 that is shown here to inhibit lymphoproliferation. The potential implications of this overlap of immunologic activities are discussed.
    Type of Medium: Online Resource
    ISSN: 0022-538X , 1098-5514
    URL: Article
    DOI: 10.1128/jvi.63.8.3257-3260.1989
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1989
    detail.hit.zdb_id: 1495529-5
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  • 3
    Online Resource
    Online Resource
    Lateral Transfer of Genes for Hexahydro-1,3,5-Trinitro-1,3,5-Triazine (RDX) Degradation
    American Society for Microbiology ; 2009
    In:  Applied and Environmental Microbiology Vol. 75, No. 10 ( 2009-05-15), p. 3258-3262
    Details
    In: Applied and Environmental Microbiology, American Society for Microbiology, Vol. 75, No. 10 ( 2009-05-15), p. 3258-3262
    Abstract: Recent studies demonstrated that degradation of the military explosive hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) by species of Rhodococcus , Gordonia , and Williamsia is mediated by a novel cytochrome P450 with a fused flavodoxin reductase domain (XplA) in conjunction with a flavodoxin reductase (XplB). Pulse field gel analysis was used to localize xplA to extrachromosomal elements in a Rhodococcus sp. and distantly related Microbacterium sp. strain MA1. Comparison of Rhodococcus rhodochrous 11Y and Microbacterium plasmid sequences in the vicinity of xplB and xplA showed near identity (6,710 of 6,721 bp). Sequencing of the associated 52.2-kb region of the Microbacterium plasmid pMA1 revealed flanking insertion sequence elements and additional genes implicated in RDX uptake and degradation.
    Type of Medium: Online Resource
    ISSN: 0099-2240 , 1098-5336
    URL: Article
    DOI: 10.1128/AEM.02396-08
    RVK:
    WA 15000
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2009
    detail.hit.zdb_id: 223011-2
    detail.hit.zdb_id: 1478346-0
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    Correlation between cag Pathogenicity Island Composition and Helicobacter pylori- Associated Gastroduodenal Disease
    American Society for Microbiology ; 2003
    In:  Infection and Immunity Vol. 71, No. 11 ( 2003-11), p. 6573-6581
    Details
    In: Infection and Immunity, American Society for Microbiology, Vol. 71, No. 11 ( 2003-11), p. 6573-6581
    Abstract: Helicobacter pylori infection is associated with a variety of outcomes ranging from seemingly asymptomatic coexistence to peptic ulcer disease and gastric cancer. The cag pathogenicity island (PAI) contains genes associated with a more aggressive phenotype and has been suggested to be a determinant of severe disease outcome. The cagA gene has served as a marker for the cag PAI. However, the presence of this single gene does not necessarily indicate the presence of a complete set of cag PAI genes. We have analyzed the composition of the cag PAI in 66 clinical isolates obtained from patients with duodenal ulcer, gastric cancer, and nonulcer dyspepsia. Hybridization of DNA to microarrays containing all the genes of the cag PAI showed that 76 and 9% of the strains contained all or none of the cag PAI genes, respectively. Partial deletions of the cag PAI were found in 10 isolates (15%), of which 3 were cagA negative. The ability to induce interleukin-8 (IL-8) production in AGS cells was correlated to the presence of a complete cag PAI. Strains carrying only parts of the island induced IL-8 at levels significantly lower than those induced by cag PAI-positive isolates. The presence of an intact cag PAI correlates with development of more severe pathology, and such strains were found more frequently in patients with severe gastroduodenal disease (odds ratio, 5.13; 95% confidence interval, 1.5 to 17.4). Partial deletions of the cag PAI appear to be sufficient to render the organism less pathogenic.
    Type of Medium: Online Resource
    ISSN: 0019-9567 , 1098-5522
    URL: Article
    DOI: 10.1128/IAI.71.11.6573-6581.2003
    RVK:
    XA 10000
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2003
    detail.hit.zdb_id: 1483247-1
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  • 5
    Online Resource
    Online Resource
    Oxidation of Chloroform in an Aerobic Soil Exposed to Natural Gas
    American Society for Microbiology ; 1986
    In:  Applied and Environmental Microbiology Vol. 52, No. 1 ( 1986-07), p. 203-205
    Details
    In: Applied and Environmental Microbiology, American Society for Microbiology, Vol. 52, No. 1 ( 1986-07), p. 203-205
    Abstract: Acclimation of a sandy soil to an air-natural gas mixture stimulated the biological oxidation of chloroform to carbon dioxide. Acetylene and methane inhibited chloroform oxidation. Chloroform oxidation continued up to 31 days in the absence of methane. Chloroform oxidation rates increased at chloroform concentrations up to 5 μg g of soil -1 .
    Type of Medium: Online Resource
    ISSN: 0099-2240 , 1098-5336
    URL: Article
    DOI: 10.1128/aem.52.1.203-205.1986
    RVK:
    WA 15000
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1986
    detail.hit.zdb_id: 223011-2
    detail.hit.zdb_id: 1478346-0
    SSG: 12
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  • 6
    Online Resource
    Online Resource
    Identification of a Dynein Interacting Domain in the Papillomavirus Minor Capsid Protein L2
    American Society for Microbiology ; 2006
    In:  Journal of Virology Vol. 80, No. 13 ( 2006-07), p. 6691-6696
    Details
    In: Journal of Virology, American Society for Microbiology, Vol. 80, No. 13 ( 2006-07), p. 6691-6696
    Abstract: Papillomaviruses enter cells via endocytosis (H. C. Selinka et al., Virology 299:279-287, 2002). After egress from endosomes, the minor capsid protein L2 accompanies the viral DNA to the nucleus and subsequently to the subnuclear promyelocytic leukemia protein bodies (P. M. Day et al., Proc. Natl. Acad. Sci. USA 101:14252-14257, 2004), suggesting that this protein may be involved in the intracytoplasmic transport of the viral genome. We now demonstrate that the L2 protein is able to interact with the microtubule network via the motor protein dynein. L2 protein was found attached to microtubules after uncoating of incoming human papillomavirus pseudovirions. Based on immunofluorescence and coimmunoprecipitation analyses, the L2 region interacting with dynein is mapped to the C-terminal 40 amino acids. Mutations within this region abrogating the L2/dynein interaction strongly reduce the infectivity of pseudoviruses, indicating that this interaction mediates the minus-end-directed transport of the viral genome along microtubules towards the nucleus.
    Type of Medium: Online Resource
    ISSN: 0022-538X , 1098-5514
    URL: Article
    DOI: 10.1128/JVI.00057-06
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2006
    detail.hit.zdb_id: 1495529-5
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  • 7
    Online Resource
    Online Resource
    Comparison of radioimmunoassay and enzyme immunoassay methods in determining gentamicin pharmacokinetic parameters and dosages
    American Society for Microbiology ; 1982
    In:  Antimicrobial Agents and Chemotherapy Vol. 22, No. 4 ( 1982-10), p. 648-651
    Details
    In: Antimicrobial Agents and Chemotherapy, American Society for Microbiology, Vol. 22, No. 4 ( 1982-10), p. 648-651
    Abstract: Radioimmunoassay and enzyme immunoassay methods for analysis of serum gentamicin levels have been shown to be comparable. The purpose of this study was to determine if serum concentration-time data from the same patient assayed by radioimmunoassay and enzyme immunoassay would provide the same estimates for half-life, elimination rate constant, distribution volume, drug clearance, and gentamicin dose. A total of 103 pre- and postinfusion serum samples were obtained from 32 patients. The samples were divided and assayed by radioimmunoassay and enzyme immunoassay. Serum concentration-time data were fitted to a one-compartment model, and kinetic calculations were performed using the method of Sawchuk et al. (Clin. Pharmacol. Ther. 21:362-369, 1977). While good correlation was established between the two assay methods, significant (P less than 0.05) mean differences were seen in distribution volume (25%), gentamicin clearance (15%), and half-life (11%), using the quantitative data from both methods. Because of differences noted in these pharmacokinetic parameters, significant differences were also noted in dosage calculations. We conclude that there are differences in the pharmacokinetic parameters obtained using results from the radioimmunoassay and enzyme immunoassay. These differences also translate into significant differences between dosage recommendations when individualization of the gentamicin regimen is attempted.
    Type of Medium: Online Resource
    ISSN: 0066-4804 , 1098-6596
    URL: Article
    DOI: 10.1128/AAC.22.4.648
    RVK:
    XA 24552
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1982
    detail.hit.zdb_id: 1496156-8
    SSG: 12
    SSG: 15,3
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  • 8
    Online Resource
    Online Resource
    Comparison of Radioimmunoassay and Enzyme Immunoassay Methods in Determining Gentamicin Pharmacokinetic Parameters and Dosages
    American Society for Microbiology ; 1983
    In:  Antimicrobial Agents and Chemotherapy Vol. 23, No. 3 ( 1983-03), p. 513-513
    Details
    In: Antimicrobial Agents and Chemotherapy, American Society for Microbiology, Vol. 23, No. 3 ( 1983-03), p. 513-513
    Type of Medium: Online Resource
    ISSN: 0066-4804 , 1098-6596
    URL: Article
    DOI: 10.1128/AAC.23.3.513-a
    RVK:
    XA 24552
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1983
    detail.hit.zdb_id: 1496156-8
    SSG: 12
    SSG: 15,3
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  • 9
    Online Resource
    Online Resource
    The Encapsidated Genome of Microplitis demolitor Bracovirus Integrates into the Host Pseudoplusia includens
    American Society for Microbiology ; 2011
    In:  Journal of Virology Vol. 85, No. 22 ( 2011-11-15), p. 11685-11696
    Details
    In: Journal of Virology, American Society for Microbiology, Vol. 85, No. 22 ( 2011-11-15), p. 11685-11696
    Abstract: Polydnaviruses (PDVs) are symbionts of parasitoid wasps that function as gene delivery vehicles in the insects (hosts) that the wasps parasitize. PDVs persist in wasps as integrated proviruses but are packaged as circularized and segmented double-stranded DNAs into the virions that wasps inject into hosts. In contrast, little is known about how PDV genomic DNAs persist in host cells. Microplitis demolitor carries Microplitis demolitor bracovirus (MdBV) and parasitizes the host Pseudoplusia includens . MdBV infects primarily host hemocytes and also infects a hemocyte-derived cell line from P. includens called CiE1 cells. Here we report that all 15 genomic segments of the MdBV encapsidated genome exhibited long-term persistence in CiE1 cells. Most MdBV genes expressed in hemocytes were persistently expressed in CiE1 cells, including members of the glc gene family whose products transformed CiE1 cells into a suspension culture. PCR-based integration assays combined with cloning and sequencing of host-virus junctions confirmed that genomic segments J and C persisted in CiE1 cells by integration. These genomic DNAs also rapidly integrated into parasitized P. includens . Sequence analysis of wasp-viral junction clones showed that the integration of proviral segments in M. demolitor was associated with a wasp excision/integration motif (WIM) known from other bracoviruses. However, integration into host cells occurred in association with a previously unknown domain that we named the host integration motif (HIM). The presence of HIMs in most MdBV genomic DNAs suggests that the integration of each genomic segment into host cells occurs through a shared mechanism.
    Type of Medium: Online Resource
    ISSN: 0022-538X , 1098-5514
    URL: Article
    DOI: 10.1128/JVI.05726-11
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2011
    detail.hit.zdb_id: 1495529-5
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  • 10
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    Online Resource
    Undergraduates Phenotyping Arabidopsis Knockouts in a Course-Based Undergraduate Research Experience: Exploring Plant Fitness and Vigor Using Quantitative Phenotyping Methods
    American Society for Microbiology ; 2019
    In:  Journal of Microbiology & Biology Education Vol. 20, No. 2 ( 2019-01)
    Details
    In: Journal of Microbiology & Biology Education, American Society for Microbiology, Vol. 20, No. 2 ( 2019-01)
    Abstract: We present a curriculum description, an initial student outcome investigation, and sample scientific results for a representative Course-Based Undergraduate Research Experience (CURE) that is part of the “Undergraduates Phenotyping Arabidopsis Knockouts” (unPAK) network. CUREs in the unPAK network characterize quantitative phenotypes of the model plant Arabidopsis from across environments to uncover connections between genotype and phenotype. Students in unPAK CUREs grow plants in a replicated block design and make quantitative measurements throughout the semester. This CURE enables students to answer plant science questions that draw from fields such as environmental science, genetics, ecology, and evolution. Findings indicate that this experience provides students with opportunities to make relevant scientific discoveries. Eighty percent of student datasets produced from the CURE met criteria for inclusion in the project database, indicative of student learning in data collection and analysis of quantitative plant traits. Student datasets uncovered novel effects of mutation on plant form. In addition, students’ science self-efficacy increased as a result of course participation, and faculty feedback on course implementation was positive. We present unPAK as a new network that supports CUREs and research experiences focused on collecting biological data made publicly available to the scientific community. The unPAK CUREs can be tailored to address instructor interests or pedagogical needs while involving students in research investigating quantitative plant phenotypes.
    Type of Medium: Online Resource
    ISSN: 1935-7877 , 1935-7885
    URL: Article
    DOI: 10.1128/jmbe.v20i2.1650
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2019
    detail.hit.zdb_id: 2560245-7
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