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  • 1
    Online Resource
    Online Resource
    iTRAQ-Based Quantitative Proteomics Analysis Reveals the Mechanism Underlying the Weakening of Carbon Metabolism in Chlorotic Tea Leaves
    MDPI AG ; 2018
    In:  International Journal of Molecular Sciences Vol. 19, No. 12 ( 2018-12-07), p. 3943-
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 19, No. 12 ( 2018-12-07), p. 3943-
    Abstract: To uncover mechanism of highly weakened carbon metabolism in chlorotic tea (Camellia sinensis) plants, iTRAQ (isobaric tags for relative and absolute quantification)-based proteomic analyses were employed to study the differences in protein expression profiles in chlorophyll-deficient and normal green leaves in the tea plant cultivar “Huangjinya”. A total of 2110 proteins were identified in “Huangjinya”, and 173 proteins showed differential accumulations between the chlorotic and normal green leaves. Of these, 19 proteins were correlated with RNA expression levels, based on integrated analyses of the transcriptome and proteome. Moreover, the results of our analysis of differentially expressed proteins suggested that primary carbon metabolism (i.e., carbohydrate synthesis and transport) was inhibited in chlorotic tea leaves. The differentially expressed genes and proteins combined with photosynthetic phenotypic data indicated that 4-coumarate-CoA ligase (4CL) showed a major effect on repressing flavonoid metabolism, and abnormal developmental chloroplast inhibited the accumulation of chlorophyll and flavonoids because few carbon skeletons were provided as a result of a weakened primary carbon metabolism. Additionally, a positive feedback mechanism was verified at the protein level (Mg chelatase and chlorophyll b reductase) in the chlorophyll biosynthetic pathway, which might effectively promote the accumulation of chlorophyll b in response to the demand for this pigment in the cells of chlorotic tea leaves in weakened carbon metabolism.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms19123943
    Language: English
    Publisher: MDPI AG
    Publication Date: 2018
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    Lipidomics Analysis of Tea Leaves Cultured in Hydroponics Reveals That High Nitrogen Application Decreases Tea Plant Resistance to Ultraviolet Radiation
    MDPI AG ; 2022
    In:  Horticulturae Vol. 8, No. 8 ( 2022-08-11), p. 724-
    Details
    In: Horticulturae, MDPI AG, Vol. 8, No. 8 ( 2022-08-11), p. 724-
    Abstract: Lipid composition in tea leaves is significantly affected by ultraviolet (UV) radiation and nitrogen levels. Here, to reveal the response of lipids in tea plants (Camellia sinensis L.) to the interaction between nitrogen and UV radiation, we treated tea plants with three gradients of UV radiation under two nitrogen levels by the hydroponic method. Lipidomics results show that ultraviolet radiation can decrease neutral lipid (TAG) and increase membrane lipids (including PC, PE, and PG) under hydroponic conditions, indicating that tea plants could survive UV radiation by decomposing TAG to avoid damaging cells. In addition, the accumulation of phospholipids and galactolipids may be related to avoiding UV damage and enhancing photosynthesis in tea plants under UV radiation. Furthermore, the response of lipid components to UV radiation in tea plants under low nitrogen conditions is significantly lower than that under high nitrogen conditions, which suggests that excessive nitrogen application may reduce the resistance of tea plants to UV radiation. This study provides a theoretical basis for optimizing cultivation measures based on tea UV resistance.
    Type of Medium: Online Resource
    ISSN: 2311-7524
    URL: Article
    DOI: 10.3390/horticulturae8080724
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2813983-5
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  • 3
    Online Resource
    Online Resource
    Broadband Microwave Photonic Channelizer with 18 Channels Based on Acousto-Optic Frequency Shifter
    MDPI AG ; 2023
    In:  Photonics Vol. 10, No. 2 ( 2023-01-20), p. 107-
    Details
    In: Photonics, MDPI AG, Vol. 10, No. 2 ( 2023-01-20), p. 107-
    Abstract: A microwave photonic channelizer can achieve instantaneous reception of ultra-wideband signals and effectively avoid electronic bottleneck; therefore, it can be perfectly applied to a wideband radar system and electronic warfare. In channelization schemes based on an optical frequency comb (OFC), the number of comb lines usually depends on that of the sub-channels. In order to improve the utilization rate of the comb lines of OFC, we propose a scheme to shift the frequency of OFC by using an acousto-optic frequency shifter (AOFS), which can obtain three times the number of sub-channels of the comb lines of an OFC. In order to simplify the experiment, only a three-line OFC is used in the experiment. A three-line local oscillator (LO) OFC is frequency-shifted up and down by two AOFSs, and nine optical LO signals with different frequencies are obtained, thereby realizing the simultaneous reception of eighteen sub-channels. The proposed scheme enjoys a large number of sub-channels and minimal channel crosstalk. Experimental results demonstrate that a 9-GHz bandwidth RF signal covering 10–19 GHz is divided into 18 sub-channels with a sub-bandwidth of 500 MHz. The image rejection ratio of the sub-channels is about 23 dB, and the spurious-free dynamic range (SFDR) of the receiver can reach 98 dB·Hz2/3.
    Type of Medium: Online Resource
    ISSN: 2304-6732
    URL: Article
    DOI: 10.3390/photonics10020107
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2770002-1
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  • 4
    Online Resource
    Online Resource
    Metagenome-Assembled Genome of Cyanocohniella sp. LLY from the Cyanosphere of Llayta, an Edible Andean Cyanobacterial Macrocolony
    MDPI AG ; 2022
    In:  Microorganisms Vol. 10, No. 8 ( 2022-07-27), p. 1517-
    Details
    In: Microorganisms, MDPI AG, Vol. 10, No. 8 ( 2022-07-27), p. 1517-
    Abstract: Cyanobacterial macrocolonies known as Llayta are found in Andean wetlands and have been consumed since pre-Columbian times in South America. Macrocolonies of filamentous cyanobacteria are niches for colonization by other microorganisms. However, the microbiome of edible Llayta has not been explored. Based on a culture-independent approach, we report the presence, identification, and metagenomic genome reconstruction of Cyanocohniella sp. LLY associated to Llayta trichomes. The assembled genome of strain LLY is now available for further inquiries and may be instrumental for taxonomic advances concerning this genus. All known members of the Cyanocohniella genus have been isolated from salty European habitats. A biogeographic gap for the Cyanocohniella genus is partially filled by the existence of strain LLY in Andes Mountains wetlands in South America as a new habitat. This is the first genome available for members of this genus. Genes involved in primary and secondary metabolism are described, providing new insights regarding the putative metabolic capabilities of Cyanocohniella sp. LLY.
    Type of Medium: Online Resource
    ISSN: 2076-2607
    URL: Article
    DOI: 10.3390/microorganisms10081517
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2720891-6
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  • 5
    Online Resource
    Online Resource
    Metagenomics of Atacama Lithobiontic Extremophile Life Unveils Highlights on Fungal Communities, Biogeochemical Cycles and Carbohydrate-Active Enzymes
    MDPI AG ; 2019
    In:  Microorganisms Vol. 7, No. 12 ( 2019-11-27), p. 619-
    Details
    In: Microorganisms, MDPI AG, Vol. 7, No. 12 ( 2019-11-27), p. 619-
    Abstract: Halites, which are typically found in various Atacama locations, are evaporitic rocks that are considered as micro-scaled salterns. Both structural and functional metagenomic analyses of halite nodules were performed. Structural analyses indicated that the halite microbiota is mainly composed of NaCl-adapted microorganisms. In addition, halites appear to harbor a limited diversity of fungal families together with a biodiverse collection of protozoa. Functional analysis indicated that the halite microbiome possesses the capacity to make an extensive contribution to carbon, nitrogen, and sulfur cycles, but possess a limited capacity to fix nitrogen. The halite metagenome also contains a vast repertory of carbohydrate active enzymes (CAZY) with glycosyl transferases being the most abundant class present, followed by glycosyl hydrolases (GH). Amylases were also present in high abundance, with GH also being identified. Thus, the halite microbiota is a potential useful source of novel enzymes that could have biotechnological applicability. This is the first metagenomic report of fungi and protozoa as endolithobionts of halite nodules, as well as the first attempt to describe the repertoire of CAZY in this community. In addition, we present a comprehensive functional metagenomic analysis of the metabolic capacities of the halite microbiota, providing evidence for the first time on the sulfur cycle in Atacama halites.
    Type of Medium: Online Resource
    ISSN: 2076-2607
    URL: Article
    DOI: 10.3390/microorganisms7120619
    Language: English
    Publisher: MDPI AG
    Publication Date: 2019
    detail.hit.zdb_id: 2720891-6
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  • 6
    Online Resource
    Online Resource
    Gas-Sensing Property of TM-MoTe2 Monolayer towards SO2, SOF2, and HF Gases
    MDPI AG ; 2022
    In:  Molecules Vol. 27, No. 10 ( 2022-05-16), p. 3176-
    Details
    In: Molecules, MDPI AG, Vol. 27, No. 10 ( 2022-05-16), p. 3176-
    Abstract: Detecting the characteristic decomposition products (SO2, SOF2, and HF) of SF6 is an effective way to diagnose the electric discharge in SF6-insulated equipment. Based on first-principles calculations, Au, Ag, and Cu were chosen as the surface modification transition metal to improve the adsorption and gas-sensing properties of MoTe2 monolayer towards SO2, SOF2, and HF gases. The results show that Au, Ag, and Cu atoms tend to be trapped by TH sites on the MoTe2 monolayer, and the binding strength increases in the order of Ag 〈 Au 〈 Cu. In gas adsorption, the moderate adsorption energy provides the basis that the TM-MoTe2 monolayer can be used as gas-sensing material for SO2, SOF2, and HF. The conductivity of the adsorption system changes significantly. The conductivity decreases upon gases adsorption on TM-MoTe2 monolayer, except the conductivity of Ag-MoTe2 monolayer increases after interacting with SOF2 gas.
    Type of Medium: Online Resource
    ISSN: 1420-3049
    URL: Article
    DOI: 10.3390/molecules27103176
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2008644-1
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