In:
Journal of Mass Spectrometry, Wiley, Vol. 57, No. 1 ( 2022-01)
Abstract:
This study aimed to establish a robust, simple method to detect 25‐hydroxyvitamin D 3 (25(OH)D 3 ), 25‐hydroxyvitamin D 2 (25(OH)D 2 ), 1,25‐dihydroxyvitamin D 3 (1,25(OH) 2 D 3 ), 1,25‐dihydroxyvitamin D 2 (1,25(OH) 2 D 2 ), 24,25‐dihydroxyvitamin D 3 (24,25(OH) 2 D 3 ), and 24,25‐dihydroxyvitamin D 2 (24,25(OH) 2 D 2 ) simultaneously with efficient separation of 3‐epi 25(OH)D 3 , 3‐epi 24,25(OH) 2 D 3 , 23R,25(OH) 2 D 3 , and 4β,25‐dihydroxyvitamin D 3 (4β,25(OH) 2 D 3 ) by liquid chromatography‐tandem mass spectrometry (LC‐MS/MS). Method This method was validated according to procedures established by Clinical and Laboratory Standards Institute (CLSI) and then applied in healthy population to determine the distribution of the vitamin D metabolites by LC‐MS/MS. Results The total‐run CV% of 25(OH)D 3 , 25(OH)D 2 , 24,25(OH) 2 D 3 , 24,25(OH) 2 D 2 , 1,25(OH) 2 D 3 , and 1,25(OH) 2 D 2 were 6.30%–8.40%, 5.00%–8.40%, 5.90%–9.00%, 5.60%–9.00%, 5.60%–8.00%, and 7.00%–9.70%, respectively. The linearity correlation coefficients r of these six vitamin D metabolites were 〉 0.99. The matrix effects of 25(OH)D 3 , 25(OH)D 2 , 24,25(OH) 2 D 3 , 24,25(OH) 2 D 2 , 1,25(OH) 2 D 3 , and 1,25(OH) 2 D 2 were 90.6%–103.3%, 97.3%–106.3%, 90.7%–106.3%, 100.7%–114.5%, 97.9%–104.6%, and 97.0%–111.0%. The trueness values of 25(OH)D 3 , 25(OH)D 2 , and 24,25(OH) 2 D 3 were 93.8%–103.0%, 101.0%, and 96.3%–100%, respectively. Conclusion This study successfully established an efficient, accurate, robust method for simultaneous measurement of serum 25(OH)D, 1,25(OH) 2 D, and 24,25(OH) 2 D by LC‐MS/MS with efficient separation of 3‐epi analogs, 23R,25(OH) 2 D 3 , and 4β,25(OH) 2 D 3 .
Type of Medium:
Online Resource
ISSN:
1076-5174
,
1096-9888
Language:
English
Publisher:
Wiley
Publication Date:
2022
detail.hit.zdb_id:
2197367-2
detail.hit.zdb_id:
1472468-6
detail.hit.zdb_id:
7414-7
SSG:
11
SSG:
12
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