In:
Evidence-Based Complementary and Alternative Medicine, Hindawi Limited, Vol. 2011 ( 2011), p. 1-9
Abstract:
This study demonstrated that many apoptotic signaling pathways, such as Rho family, PKC family, MAP kinase family, and mitochondria-mediated apoptotic pathway, were triggered by Lonicera japonica extracts and irradiation in CH27 cells. Rottlerin, a PKC δ -selective inhibitor, reversed the photoactivated Lonicera japonica extract-induced decrease in PKC δ protein expression and change in cell morphology in this study. In addition, rottlerin inhibited the photoactivated Lonicera japonica -induced decrease in protein expression of Ras, ERK, p38, PKC α , and PKC ε , which are the kinases of prosurvival signaling pathway. We also demonstrated that pretreatment with rottlerin prevented actin microfilaments and microtubules from damage during the photoactivated Lonicera japonica -induced CH27 cell death. Furthermore, the promotion of the cytoskeleton-related signaling cascade following rottlerin by upregulation of cytoskeleton-related mediators (p38, HSP27, FAK, paxillin, and tubulin) and molecules of downstream of F-actin (mitochondria-mediated apoptosis pathway) reduces CH27 cell death, indicating that cytoskeleton is the potential target in the photoactivated Lonicera japonica extract-induced photokilling of CH27 cells.
Type of Medium:
Online Resource
ISSN:
1741-427X
,
1741-4288
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2011
detail.hit.zdb_id:
2148302-4
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