In:
Applied and Environmental Microbiology, American Society for Microbiology, Vol. 69, No. 12 ( 2003-12), p. 6987-6993
Abstract:
Pseudomonas sp. strain ADP uses the herbicide atrazine as the sole nitrogen source.
We have devised a simple atrazine degradation assay to determine the effect of other nitrogen sources on the atrazine degradation pathway.
The atrazine degradation rate was greatly decreased in cells grown on nitrogen sources that support rapid growth of Pseudomonas sp.
strain ADP compared to cells cultivated on growth-limiting nitrogen sources. The presence of atrazine in addition to the nitrogen sources
did not stimulate degradation. High degradation rates obtained in the presence of ammonium plus the glutamine synthetase inhibitor MSX and
also with an Nas − mutant derivative grown on nitrate
suggest that nitrogen regulation operates by sensing intracellular levels of some key nitrogen-containing metabolite. Nitrate amendment in
soil microcosms resulted in decreased atrazine mineralization by the wild-type strain but not by the Nas − mutant. This
suggests that, although nitrogen repre ssion of the atrazine catabolic
pathway may have a strong impact on atrazine biodegradation in nitrogen-fertilized soils, the use of selected mutant variants may
contribute to overcoming this limitation.
Type of Medium:
Online Resource
ISSN:
0099-2240
,
1098-5336
DOI:
10.1128/AEM.69.12.6987-6993.2003
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
2003
detail.hit.zdb_id:
223011-2
detail.hit.zdb_id:
1478346-0
SSG:
12
Bookmarklink