In:
Journal of General Virology, Microbiology Society, Vol. 83, No. 10 ( 2002-10-01), p. 2361-2365
Kurzfassung:
Virion host shutoff (vhs)-deficient herpes simplex virus (HSV) was tested as a therapeutic vaccine in a mouse model of UV light-induced recurrent herpetic stromal keratitis. Four weeks after primary corneal infection, mice were vaccinated intraperitoneally with vhs − vaccine or control. Four weeks after vaccination, the eyes of latently infected mice were UV-B irradiated to induce recurrent virus shedding and disease. Post-irradiation corneal opacity in latently infected, vhs − -vaccinated mice was significantly reduced compared to control-vaccinated mice ( P =0·007 to 0·035). The incidence and duration of recurrent virus shedding were the same in both groups. Antibody titres were increased ( P =0·05) and delayed type hypersensitive responses were unaffected by vhs − vaccination. Combined with studies using different vaccination timing and vhs − genotypes, these data suggest that deletion of vhs is a useful strategy in the development of a therapeutic HSV vaccine, and that temporal and genetic factors influence vaccination outcome.
Materialart:
Online-Ressource
ISSN:
0022-1317
,
1465-2099
DOI:
10.1099/0022-1317-83-10-2361
Sprache:
Englisch
Verlag:
Microbiology Society
Publikationsdatum:
2002
ZDB Id:
2007065-2
SSG:
12
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