In:
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 94, No. 6 ( 1997-03-18), p. 2513-2517
Abstract:
Pluripotent hemopoietic stem cells (P-HSCs) were thought to be c-kit + , but recent reports indicate that they are c-kit low . In the present report, we provide evidence using Ly5 congenic mice that P-HSCs are c-kit 〈 low . Lineage-negative (Lin − )/CD71 − cells among bone marrow cells (BMCs) from C57BL/6 Ly5.1 mice were separated into major histocompatibility complex class I high (class I high )/c-kit low and class I high /c-kit 〈 low populations. Each population (500 cells) was transplanted into lethally (9.0 Gy) irradiated C57BL/6 Ly5.2 congenic mice along with Ly5.2 (2 × 10 5 ) compromised cells. Donor-derived Ly5.1 + cells were detected 6 months after transplantation in primary recipients reconstituted with either class I high /c-kit low or class I high /c-kit 〈 low cells. BMCs (1 × 10 6 ) from the primary recipients were further transplanted into secondary recipients (Ly5.2 mice) to assess their long term repopulating activity. Six months after bone marrow transplantation, Ly5.1 + cells in all lineages were detected only in secondary recipients that had been given BMCs from the primary recipients reconstituted with class I high /c-kit 〈 low cells but not in cells that were class I high /c-kit low . When the BMCs (1 × 10 6 ) of these secondary recipients were further transplanted into tertiary recipients, all tertiary recipients that had been given BMCs from the secondary recipients originally reconstituted with Lin − /CD71 − /class I high /c-kit low cells died within 10 days whereas all six tertiary recipients originally reconstituted with Lin − /CD71 − /class I high /c-kit 〈 low cells showed donor (Ly5.1 + )-derived cells in their peripheral blood. In the single tertiary recipient that was killed, donor-derived T cells, B cells, macrophages, and granulocytes also were detected in several major hematolymphoid organs. The remaining five mice continue to survive more than 6 months after the tertiary bone marrow transplantation.
Type of Medium:
Online Resource
ISSN:
0027-8424
,
1091-6490
DOI:
10.1073/pnas.94.6.2513
Language:
English
Publisher:
Proceedings of the National Academy of Sciences
Publication Date:
1997
detail.hit.zdb_id:
209104-5
detail.hit.zdb_id:
1461794-8
SSG:
11
SSG:
12
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