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  • 1
    In: Biological Chemistry, Walter de Gruyter GmbH, Vol. 381, No. 7 ( 2000-01-04)
    Type of Medium: Online Resource
    ISSN: 1431-6730
    Language: Unknown
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2000
    detail.hit.zdb_id: 1466062-3
    SSG: 12
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  • 2
    In: Bio-Algorithms and Med-Systems, Walter de Gruyter GmbH, Vol. 11, No. 2 ( 2015-6-15), p. 107-111
    Abstract: Background: The Bavarian Virtual University (BVU) offers resources to develop and implement e-learning courses and allows open access to information and provides the capacity to share information. Students of Bavarian universities can participate easily in these courses. Thus, the barriers to enter a university are now much lower. E-learning will be supported by the use of the learning and authoring software CASUS ® . It optimises the creation of virtual patients and guarantees a common standard and a high quality throughout the whole e-learning course. Methods: This paper describes the application process of proposals at BVU and the development process of online courses with CASUS ® using the example of “General Practice”. The conceptual structure of the program and its specific rationale is presented herein. In addition, the number of participating students is analysed when the online course is integrated in the curriculum or when it is not. Results: The e-learning course General Practice includes a total of 48 scenarios. They focus on the heuristics in General Practice of prevention and primary care. If the online courses are integrated in the curriculum, the number of students participating in e-learning courses increases. The integration of virtual patients (VPs) into practical training may improve the perceived benefit. Probably, there is no need for examinations to prove students’ knowledge if they can apply contents in the practical training. To answer this question, an evaluation of students’ and teachers’ perspective is needed. In addition, it is challenging to define one level of education for students of different universities. Hence, new methods are needed to develop and evaluate generally accepted standards that fit all participating universities and their students. Conclusions: The application of proposals at BVU and creating VPs with the learning and authoring system CASUS ® can be highly recommended to other universities, as well as the establishment of organisations like BVU in other countries.
    Type of Medium: Online Resource
    ISSN: 1896-530X , 1895-9091
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2015
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  • 3
    Online Resource
    Online Resource
    Walter de Gruyter GmbH ; 1967
    In:  Radiochimica Acta Vol. 8, No. 3 ( 1967-12-1), p. 132-142
    In: Radiochimica Acta, Walter de Gruyter GmbH, Vol. 8, No. 3 ( 1967-12-1), p. 132-142
    Type of Medium: Online Resource
    ISSN: 2193-3405 , 0033-8230
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 1967
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  • 4
    In: Biological Chemistry, Walter de Gruyter GmbH, Vol. 395, No. 7-8 ( 2014-7-1), p. 779-789
    Abstract: Ternary lipid mixtures composed of cholesterol, saturated (frequently with sphingosine backbone), and unsaturated phospholipids show stable phase separation and are often used as model systems of lipid rafts. Yet, their ability to reproduce raft properties and function is still debated. We investigated the properties and functional aspects of three lipid raft model systems of varying degrees of biological relevance – PSM/POPC/Chol, DPPC/POPC/Chol, and DPPC/DOPC/Chol – using 2 H solid-state nuclear magnetic resonance (NMR) spectroscopy, fluorescence microscopy, and atomic force microscopy. While some minor differences were observed, the general behavior and properties of all three model mixtures were similar to previously investigated influenza envelope lipid membranes, which closely mimic the lipid composition of biological membranes. For the investigation of the functional aspects, we employed the human N-Ras protein, which is posttranslationally modified by two lipid modifications that anchor the protein to the membrane. It was previously shown that N-Ras preferentially resides in liquid-disordered domains and exhibits a time-dependent accumulation in the domain boundaries of influenza envelope lipid membranes. For all three model mixtures, we observed the same membrane partitioning behavior for N-Ras. Therefore, we conclude that even relatively simple models of raft membranes are able to reproduce many of their specific properties and functions.
    Type of Medium: Online Resource
    ISSN: 1437-4315 , 1431-6730
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2014
    detail.hit.zdb_id: 1466062-3
    SSG: 12
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  • 5
    In: Biological Chemistry, Walter de Gruyter GmbH, Vol. 402, No. 11 ( 2021-10-26), p. 1427-1440
    Abstract: Glycosaminoglycans (GAGs) are essential functional components of the extracellular matrix (ECM). Artificial GAGs like sulfated hyaluronan (sHA) exhibit pro-osteogenic properties and boost healing processes. Hence, they are of high interest for supporting bone regeneration and wound healing. Although sulfated GAGs (sGAGs) appear intracellularly, the knowledge about intracellular effects and putative interaction partners is scarce. Here we used an affinity-purification mass spectrometry-based (AP-MS) approach to identify novel and particularly intracellular sGAG-interacting proteins in human bone marrow stromal cells (hBMSC). Overall, 477 proteins were found interacting with at least one of four distinct sGAGs. Enrichment analysis for protein localization showed that mainly intracellular and cell-associated interacting proteins were identified. The interaction of sGAG with α2-macroglobulin receptor-associated protein (LRPAP1), exportin-1 (XPO1), and serine protease HTRA1 (HTRA1) was confirmed in reverse assays. Consecutive pathway and cluster analysis led to the identification of biological processes, namely processes involving binding and processing of nucleic acids, LRP1-dependent endocytosis, and exosome formation. Respecting the preferentially intracellular localization of sGAG in vesicle-like structures, also the interaction data indicate sGAG-specific modulation of vesicle-based transport processes. By identifying many sGAG-specific interacting proteins, our data provide a resource for upcoming studies aimed at molecular mechanisms and understanding of sGAG cellular effects.
    Type of Medium: Online Resource
    ISSN: 1431-6730 , 1437-4315
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2021
    detail.hit.zdb_id: 1466062-3
    SSG: 12
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  • 6
    Online Resource
    Online Resource
    Walter de Gruyter GmbH ; 2005
    In:  Biological Chemistry Vol. 386, No. 12 ( 2005-12-01), p. 1219-1238
    In: Biological Chemistry, Walter de Gruyter GmbH, Vol. 386, No. 12 ( 2005-12-01), p. 1219-1238
    Abstract: Small non-coding RNAs (sRNAs) have attracted considerable attention as an emerging class of gene expression regulators. In bacteria, a few regulatory RNA molecules have long been known, but the extent of their role in the cell was not fully appreciated until the recent discovery of hundreds of potential sRNA genes in the bacterium Escherichia coli . Orthologs of these E. coli sRNA genes, as well as unrelated sRNAs, were also found in other bacteria. Here we review the disparate experimental approaches used over the years to identify sRNA molecules and their genes in prokaryotes. These include genome-wide searches based on the biocomputational prediction of non-coding RNA genes, global detection of non-coding transcripts using microarrays, and shotgun cloning of small RNAs (RNomics). Other sRNAs were found by either co-purification with RNA-binding proteins, such as Hfq or CsrA/RsmA, or classical cloning of abundant small RNAs after size fractionation in polyacrylamide gels. In addition, bacterial genetics offers powerful tools that aid in the search for sRNAs that may play a critical role in the regulatory circuit of interest, for example, the response to stress or the adaptation to a change in nutrient availability. Many of the techniques discussed here have also been successfully applied to the discovery of eukaryotic and archaeal sRNAs.
    Type of Medium: Online Resource
    ISSN: 1431-6730 , 1437-4315
    Language: Unknown
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2005
    detail.hit.zdb_id: 1466062-3
    SSG: 12
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  • 7
    Online Resource
    Online Resource
    Walter de Gruyter GmbH ; 2010
    In:  Journal of Pediatric Endocrinology and Metabolism Vol. 23, No. 9 ( 2010-01)
    In: Journal of Pediatric Endocrinology and Metabolism, Walter de Gruyter GmbH, Vol. 23, No. 9 ( 2010-01)
    Type of Medium: Online Resource
    ISSN: 0334-018X , 2191-0251
    Language: Unknown
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2010
    detail.hit.zdb_id: 2583847-7
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  • 8
    Online Resource
    Online Resource
    Walter de Gruyter GmbH ; 2021
    In:  Scandinavian Journal of Pain Vol. 21, No. 2 ( 2021-04-27), p. 238-246
    In: Scandinavian Journal of Pain, Walter de Gruyter GmbH, Vol. 21, No. 2 ( 2021-04-27), p. 238-246
    Abstract: Surgery may possibly be undermined by psychologic, psychiatric and psychosomatic problems, as long as these problems interfere with a patient’s capacity to cope with surgery adaptively. Recent studies have shown that interpersonal trauma, e.g. abuse or neglect, and its correlates are involved in the adaptation to surgery. This observation is heuristically coherent, given the respective traumatization is an interpersonal event occurring in a relationship. Notably, surgery inevitably leads to the violation of physical boundaries within a doctor-patient relationship. Based on the principles of psycho-traumatologic thinking, such a constellation is deemed qualified to activate posttraumatic symptoms in the traumatized. Method The present topical review summarizes the respective findings which point to a subgroup of patients undergoing surgery, in whom difficulty bearing tension and confiding in others may cause adaptive problems relevant to surgery. Although this theorizing is empirically substantiated primarily with respect to total knee arthroplasty (TKA), a pubmed-research reveals psychopathologic distress to occur prior to surgery beyond TKA. Likewise, posttraumatic distress occurs in large numbers in the context of several operations, including cardiac, cancer and hernia surgery. Conclusion Aspects of psychological trauma may be linked to the outcomes of general surgery, as well, e.g. biliary, hernia or appendix surgery. The mechanisms possibly involved in this process are outlined in terms of a hierarchical organization of specific anxiety and negative affect as well as in terms of psychodynamics which imply the unconscious action of psychologic defenses at their core. Implications Not least, we encourage the screening for trauma and its correlates including defenses prior to general surgery in order to identify surgical candidates at risk of, e.g. chronic postoperative pain, before the operation.
    Type of Medium: Online Resource
    ISSN: 1877-8879 , 1877-8860
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2021
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