Kooperativer Bibliotheksverbund

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Format: XXXV, 570 Seiten : , Illustrationen, Diagramme (teilweise farbig).

ISBN: 3-527-33784-9 , 978-3-527-33784-2 , 978-3-527-69023-7

Series Statement: Advanced micro and nanosystems

Additional Edition: Erscheint auch als Online-Ausgabe, EPUB ISBN 978-3-527-69025-1

Additional Edition: Erscheint auch als Online-Ausgabe, MOBI ISBN 978-3-527-69024-4

Additional Edition: Erscheint auch als Online-Ausgabe, PDF ISBN 978-3-527-69022-0

Language: English

Subjects: Engineering

Keywords: Nanotechnologie ; Miniroboter ; Mikromanipulator ; Nanobiotechnologie ; Mikrosystemtechnik ; Lehrbuch ; Lehrbuch

URL: Inhaltstext

URL: Inhaltsverzeichnis

URL: Inhaltstext

URL: Inhaltsverzeichnis

Author information: Sun, Yu 1974-

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Format: 1 online resource (xii, 371 pages) : , digital, PDF file(s).

ISBN: 9781139939751 (ebook)

Content: The first of its kind, this comprehensive resource integrates cellular mechanobiology with micro-nano techniques to provide unrivalled in-depth coverage of the field, including state-of-the-art methods, recent advances, and biological discoveries. Structured in two parts, the first part offers detailed analysis of innovative micro-nano techniques including FRET imaging, electron cryo-microscopy, micropost arrays, nanotopography devices, laser ablation, and computational image analysis. The second part of the book provides valuable insights into the most recent technological advances and discoveries in areas such as stem cell, heart, bone, brain, tumor, and fibroblast mechanobiology. Written by a team of leading experts and well-recognised researchers, this is an essential resource for students and researchers in biomedical engineering.

Note: Title from publisher's bibliographic system (viewed on 11 Nov 2015).

Additional Edition: Print version: ISBN 9781107078390

Language: English

URL: https://doi.org/10.1017/CBO9781139939751

UID:

Format: 1 online resource

ISBN: 9783527690237 , 3527690239 , 9783527690220 , 3527690220 , 9783527690251 , 3527690255 , 3527690247 , 9783527690244

Series Statement: Advanced micro & nanosystems

Content: Combining robotics with nanotechnology, this ready reference summarizes the fundamentals and emerging applications in this fascinating research field. This is the first book to introduce tools specifically designed and made for manipulating micro- and nanometer-sized objects, and presents such examples as semiconductor packaging and clinical diagnostics as well as surgery. The first part discusses various topics of on-chip and device-based micro- and nanomanipulation, including the use of acoustic, magnetic, optical or dielectrophoretic fields, while surface-driven and high-speed microfluidic.

Note: Related Titles; Title Page; Copyright; Table of Contents; About the Editors; Series Editors Preface; Preface; List of Contributors; Chapter 1: High-Speed Microfluidic Manipulation of Cells; 1.1 Introduction; 1.2 Direct Cell Manipulation; 1.3 Indirect Cell Manipulation; 1.4 Summary; Acknowledgments; References; Chapter 2: Micro and Nano Manipulation and Assembly by Optically Induced Electrokinetics; 2.1 Introduction; 2.2 Optically Induced Electrokinetic (OEK) Forces; 2.3 OEK-Based Manipulation and Assembly; 2.4 Summary; References. , Chapter 3: Manipulation of DNA by Complex Confinement Using Nanofluidic Slits3.1 Introduction; 3.2 Slitlike Confinement of DNA; 3.3 Differential Slitlike Confinement of DNA; 3.4 Experimental Studies; 3.5 Design of Complex Slitlike Devices; 3.6 Fabrication of Complex Slitlike Devices; 3.7 Experimental Conditions; 3.8 Conclusion; Disclaimer; References; Chapter 4: Microfluidic Approaches for Manipulation and Assemblyof One-Dimensional Nanomaterials; 4.1 Introduction; 4.2 Microfluidic Assembly; 4.3 Summary; References. , Chapter 5: Optically Assisted and Dielectrophoretical Manipulation of Cells and Molecules on Microfluidic Platforms5.1 Introduction; 5.2 Operating Principle and Fundamental Physics of the ODEP Platform; 5.3 Applications of the ODEP Platform; 5.4 Conclusion; References; Chapter 6: On-Chip Microrobot Driven by Permanent Magnetsfor Biomedical Applications; 6.1 On-Chip Microrobot; 6.2 Characteristics of Microrobot Actuated by Permanent Magnet; 6.3 Friction Reduction for On-Chip Robot; 6.4 Fluid Friction Reduction for On-Chip Robot; 6.5 Applications of On-Chip Robot to Cell Manipulations. , 6.6 SummaryReferences; Chapter 7: Silicon Nanotweezers for Molecules and Cells Manipulation and Characterization; 7.1 Introduction; 7.2 SNT Operation and Design; 7.3 SNT Process; 7.4 DNA Trapping and Enzymatic Reaction Monitoring; 7.5 Cell Trapping and Characterization; 7.6 General Concluding Remarks and Perspectives; Acknowledgments; References; Chapter 8: Miniaturized Untethered Tools for Surgery; 8.1 Introduction; 8.2 Macroscale Untethered Surgical Tools; 8.3 Microscale Untethered Surgical Tools; 8.4 Nanoscale Untethered Surgical Tools; 8.5 Conclusion; Acknowledgments; References. , Chapter 9: Single-Chip Scanning Probe Microscopes9.1 Scanning Probe Microscopy; 9.2 The Role of MEMS in SPM; 9.3 CMOS-MEMS Manufacturing Processes Applied to sc-SPMs; 9.4 Modeling and Design of sc-SPMs; 9.5 Imaging Results; 9.6 Conclusion; References; Chapter 10: Untethered Magnetic Micromanipulation; 10.1 Physics of Micromanipulation; 10.2 Sliding Friction and Surface Adhesion; 10.3 Fluid Dynamics Effects; 10.4 Magnetic Microrobot Actuation; 10.5 Locomotion Techniques; 10.6 Manipulation Techniques; 10.7 Conclusions and Prospects; References; Chapter 11: Microrobotic Tools for Plant Biology.

Additional Edition: Erscheint auch als: Druck-Ausgabe Micro- and nanomanipulation tools

Language: English

Keywords: Electronic books. ; Electronic books. ; Electronic books. ; Electronic books.

URL: https://onlinelibrary.wiley.com/doi/book/10.1002/9783527690237

URL: Click to View

URL: https://onlinelibrary.wiley.com/doi/book/10.1002/9783527690237

URL: https://onlinelibrary.wiley.com/doi/book/10.1002/9783527690237

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Format: 1 Online-Ressource (16 Seiten)

Content: Queuosine (Q) is a complex tRNA modification found in bacteria and eukaryotes at position 34 of four tRNAs with a GUN anticodon, and it regulates the translational efficiency and fidelity of the respective codons that differ at the Wobble position. In bacteria, the biosynthesis of Q involves two precursors, preQ0 and preQ1, whereas eukaryotes directly obtain Q from bacterial sources. The study of queuosine has been challenging due to the limited availability of high-throughput methods for its detection and analysis. Here, we have employed direct RNA sequencing using nanopore technology to detect the modification of tRNAs with Q and Q precursors. These modifications were detected with high accuracy on synthetic tRNAs as well as on tRNAs extracted from Schizosaccharomyces pombe and Escherichia coli by comparing unmodified to modified tRNAs using the tool JACUSA2. Furthermore, we present an improved protocol for the alignment of raw sequence reads that gives high specificity and recall for tRNAs ex cellulo that, by nature, carry multiple modifications. Altogether, our results show that 7-deazaguanine-derivatives such as queuosine are readily detectable using direct RNA sequencing. This advancement opens up new possibilities for investigating these modifications in native tRNAs, furthering our understanding of their biological function.

Content: Peer Reviewed

In: Oxford : Oxford Univ. Press, 51,20, Seiten 11197-11212

Language: English

DOI: 10.18452/29038

DOI: 10.1093/nar/gkad826

URN: urn:nbn:de:kobv:11-110-18452/29659-3

URL: Volltext  (kostenfrei)

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ISBN: 0-443-18845-9 , 0-443-18844-0

Language: English

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Format: VIII, 130 p. 92 illus., 63 illus. in color. , online resource.

Edition: 1st ed. 2023.

ISBN: 9783031527302

Content: This is the first book to focus on robotic reproductive cell manipulation. It provides readers with the fundamental principles underpinning robotic manipulation of reproductive cells, including sperm, oocytes, and embryos, state-of-the-art technical advances in actuation, sensing and control for cell manipulation, and emerging automated systems for reproductive cell manipulation. The methods presented in the book are generic and can be translated to manipulating other types of cells, such as cancer cells and cardiomyocytes. Robotic Manipulation of Reproductive Cells will be an essential reference for graduate students and researchers working on small-scale robotic systems for cell manipulation and characterization, healthcare professionals interested in nanoscale, microscale, milli-scale robotic techniques for clinical cell surgeries and assisted reproduction, and engineers developing small-scale robotic systems for biomedical engineering, biology, and medicine. Introduces the applications of robotic cell manipulation; Highlights advances in infertility diagnosis and treatment; Provides insightful outlook on future challenges and opportunities. .

Note: Chapter 1. Overview of robotic reproductive cell manipulation -- Chapter 2. Automated sperm analysis -- Chapter 3. Robotic sperm immobilization -- Chapter 4. Automated picoliter-resolution sperm aspiration -- Chapter 5. Robotic orientation control of linear-shaped sperm -- Chapter 6. Robotic orientation control of spherical oocytes -- Chapter 7. Piezo drill-based minimally invasive oocyte injection -- Chapter 8. Robotic embryo characterization and manipulation -- Chapter 9. Untethered robotic manipulation of reproductive cells -- Chapter 10. Future perspectives of robotic manipulation of reproductive cells.

In: Springer Nature eBook

Additional Edition: Printed edition: ISBN 9783031527296

Additional Edition: Printed edition: ISBN 9783031527319

Additional Edition: Printed edition: ISBN 9783031527326

Language: English

DOI: 10.1007/978-3-031-52730-2

URL: https://doi.org/10.1007/978-3-031-52730-2

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Format: 1 online resource (406 pages)

ISBN: 0-12-823990-5

Language: English

UID:

Format: 1 online resource (402 pages)

ISBN: 9780323952149

Note: Intro -- Robotics for Cell Manipulation and Characterization -- Copyright -- Contents -- Contributors -- Preface -- Part I: Robotic cell manipulation -- Chapter 1: Introduction of robotics for cell manipulation and characterization -- 1. Introduction -- 2. Robotic cell manipulation -- 2.1. Robotic end effectors -- 2.1.1. Micropipettes -- 2.1.2. AFM probes -- 2.1.3. Microgrippers -- 2.2. Field-driven manipulation -- 2.2.1. Magnetic manipulation -- 2.2.2. Optical manipulation -- 2.2.3. Acoustic manipulation -- 2.2.4. Electric manipulation -- 2.2.5. Fluidic manipulation -- 3. Robotic cell characterization -- 3.1. Mechanical characterization -- 3.2. Intracellular structure characterization -- 4. Summary and outlook -- References -- Chapter 2: Robotic cell injection with force sensing and control -- 1. Introduction -- 1.1. Conventional manual microinjection -- 1.2. Robotic cell microinjection -- 1.3. Force-assisted robotic cell microinjection -- 2. Microinjection of adherent cells -- 3. Microinjection of suspended cells -- 3.1. Requirement of suspended cell injection -- 3.2. Robotic microinjection system for suspended cells -- 3.3. Force-assisted robotic microinjection of suspended cells -- 4. Microforce sensors for cell microinjection -- 4.1. Vision-based force sensors -- 4.1.1. Image processing for vision-based force sensor -- 4.1.2. Cell model for vision-based force sensor -- 4.1.3. Advantages and limitations -- 4.2. Capacitive force sensors -- 4.3. Optical-based force sensors -- 4.4. Piezoresistive force sensors -- 4.5. Piezoelectric force sensors -- 4.6. Comparison of the force sensors -- 5. Current challenges of cell microinjection and future development -- 5.1. Microinjector design -- 5.2. Injection control design -- 5.3. Cell holder design -- 5.4. Penetration scheme design -- 5.5. Microinjection pipette maintenance. , 5.6. Issue of injection volume -- 6. Conclusion -- Acknowledgments -- References -- Chapter 3: Robotic orientation control and enucleation of cells -- 1. Introduction -- 2. Robotic orientation control of cells -- 2.1. Cell rotation based on magnetic fields -- 2.1.1. Noninvasive manipulation -- 2.1.2. Invasive manipulation -- 2.2. Cell rotation based on acoustic fields -- 2.2.1. Cell rotation in SAW-based devices -- 2.2.2. Cell rotation in BAW-based devices -- 2.3. Cell rotation based on AC E-field in optoelectronic tweezers -- 2.3.1. Cell rotation in a rotational AC electric field -- 2.3.2. Cell rotation in an irrotational AC electric field -- 3. Robotic enucleation of cells -- 3.1. Introduction of robotic enucleation -- 3.2. Microrobots for enucleation -- 3.3. Future directions of robotic cell enucleation -- 4. Conclusion and perspectives -- References -- Chapter 4: Robotic cell manipulation for in vitro fertilization -- 1. Introduction -- 2. Robotic end-effector alignment for sperm immobilization -- 3. Robotic rotation of sperm as deformable linear objects -- 4. Robotic orientation control of deformable oocyte -- 5. Robotic cell penetration with piezo drill -- 6. Summary -- References -- Chapter 5: Robotic cell transport for tissue engineering -- 1. Introduction -- 2. Robotic transport for cell isolating and positioning -- 2.1. Pick and place strategy -- 2.2. Wireless actuation strategy -- 3. Robotic transport for fabrication and assembly of cellular modules -- 3.1. Cell encapsulation -- 3.1.1. Photolithography -- 3.1.2. Microfluidic formation methods -- 3.2. Cellular micromodule transport for 3D tissue assembly -- 3.2.1. Mechanically actuated assembly -- 3.2.2. Field-actuated assembly -- 3.2.3. Self-assembly -- 4. Summary -- Acknowledgment -- References -- Chapter 6: Robotic cell biopsy for disease diagnosis -- 1. Introduction. , 2. Generic small cell biopsy system -- 2.1. System development -- 2.2. Cell patterning -- 2.3. Cell compression for organelle positioning -- 3. Cell biopsy process -- 3.1. Procedures of automatic organelle extraction and release -- 3.2. Motion control -- 3.3. Biological tests -- 4. Experiments -- 4.1. Material preparation -- 4.2. Organelle extraction -- 4.3. Biological tests on extracted organelles and the remaining cells -- 5. Conclusions -- References -- Chapter 7: 3D force-feedback optical tweezers for experimental biology -- 1. Robotic bio-manipulation -- 1.1. Contact methods for robotic bio-manipulation -- 1.2. External energy-fields for robotic bio-manipulation -- 2. Optical micromanipulation -- 2.1. Principle and development of optical tweezers -- 2.2. Biological applications of optical manipulation -- 2.3. Optical manipulation setups -- 2.3.1. Force measurement in optical traps -- 2.3.2. Position control in optical traps -- 3. 3D real-time force sensing in optical manipulation -- 3.1. Background and related work -- 3.2. Asynchronous time-based image sensor -- 3.3. System description -- 3.4. 3D tracking -- 4. Evaluation of tracking -- 4.1. Range and resolution -- 4.2. Robustness -- 4.3. Computational load -- 4.4. 3D haptic feedback optical tweezers -- 4.5. Haptic coupling -- 4.6. Calibration -- 5. 3D haptic experiments on biologic samples -- 5.1. Z-axis haptic feedback -- 5.2. 3D haptic exploration -- References -- Chapter 8: Magnetically driven robots for clinical treatment -- 1. Introduction -- 2. Actuating microrobots using magnetic fields -- 2.1. Gradient magnetic fields -- 2.2. Rotating magnetic fields -- 2.3. Oscillating magnetic fields -- 2.4. Other forms of magnetic fields -- 3. Clinical applications for microrobots -- 3.1. Dealing with physiological environments -- 3.2. Tracking using medical imaging devices. , 3.3. Microrobots for clinical operations -- 4. Conclusion -- References -- Part II: Robotic cell characterization -- Chapter 9: Robotic cell electrophysiological characterization for drug discovery -- 1. Introduction -- 1.1. The current state-of-the-art robotics for electrophysiology characterization -- 1.2. The patcherBot framework -- 1.3. The novel features of the patcherBot and the patcherBotpharma -- 1.4. patcherBotpharma implementation and performance -- 1.5. patcherBotPharma performance: A case study -- 2. Discussion -- References -- Chapter 10: Automated cell aspiration for genetic and mechanical analysis -- 1. Introduction -- 2. Automated cell aspiration for mechanical analysis -- 2.1. Measured cell capture -- 2.2. Pressure control in the micropipette -- 2.3. Visual detection of cellular geometric parameters -- 2.4. Mechanical analysis based on cellular deformation -- 3. Automated cell aspiration for genetic analysis -- 3.1. Visual detection of subcellular structures -- 3.2. Aspiration volume control from cells -- 4. Conclusion and outlook -- References -- Chapter 11: Cell characterization by nanonewton force sensing -- 1. Introduction -- 2. Techniques for sensing cell-generated forces -- 2.1. Traction force microscopy -- 2.2. Atomic force microscopy -- 2.3. Optical and magnetic tweezers -- 2.4. Microfabricated structures from MEMS -- 3. Internal molecular sensors for detecting cell-generated forces -- 3.1. Förster resonance energy transfer (FRET) tension sensor -- 3.2. DNA-based tension sensors -- 3.2.1. Tension gauge tethers -- 3.2.2. DNA hairpin force probes -- 4. Concluding remarks -- References -- Chapter 12: Cellular mechanical measurement by magnetic micro/nanorobots -- 1. Introduction -- 1.1. Cell mechanics -- 1.2. Techniques measuring cell mechanics: State of art -- 2. Principles of magnetic actuation. , 2.1. Magnetic materials and nanoparticles -- 2.2. Magnetic actuation on a single magnetic nanoparticle -- 3. Magnetic systems for magnetic actuation -- 4. Magnetic measurement of cellular and intracellular structure mechanics -- 4.1. Cell mechanics and rheological properties -- 4.2. Mechanical properties of nucleus -- 4.3. Mechanical properties of cytoskeleton, motor proteins, and DNA strands -- 5. Summary and outlook -- References -- Chapter 13: Nanorobotics for investigating cell mechanics based on atomic force microscopy -- 1. Background -- 2. Atomic force microscopy (AFM)-based nanomanipulator -- 3. Methodology of characterizing cellular mechanics by AFM nanomanipulator -- 4. Applications of AFM nanomanipulator in detecting cell mechanics -- 5. Combining AFM nanorobotics with micropipette for precise drug-induced cellular mechanical analysis -- 6. Summary -- Acknowledgments -- References -- Chapter 14: Robotic manipulation of zebrafish larvae for disease therapy -- 1. Introduction -- 2. Robotic transportation -- 3. Robotic immobilization -- 4. Robotic orientation -- 5. Robotic injection -- 6. Conclusion -- Acknowledgments -- References -- Chapter 15: Acoustic field techniques for cell characterization in health monitoring -- 1. Introduction -- 2. Acoustic techniques -- 2.1. Acoustic traveling wave -- 2.2. Acoustic standing wave -- 2.3. Acoustic streaming -- 2.4. Focused ultrasound -- 2.5. Holographic acoustic tweezers -- 2.6. Surface acoustic waves -- 2.7. Ultrasound imaging -- 3. Cell analysis -- 3.1. Cell separation and concentration -- 3.2. Cell patterning, cultivating, and intercellular interaction -- 3.3. Cell sonoporation, transfection, and rotation -- 3.4. Cell mechanical characterization -- 4. Current challenges and future direction -- References -- Chapter 16: Separation and characterization of cells using electrical field. , 1. Introduction.

Additional Edition: Print version: Dai, Changsheng Robotics for Cell Manipulation and Characterization San Diego : Elsevier Science & Technology,c2023 ISBN 9780323952132

Language: English

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Format: XX, 169 p. 22 illus., 14 illus. in color. , online resource.

Edition: 1st ed. 2020.

ISBN: 9789811570568

Content: This book provides in-depth analysis and guidance in the clinical diagnosis and treatment, and development of new treatments with clinical applied prospect of burn and trauma associated lung injury, and does further study on the pathological change of burn and trauma associated lung injury such as inhalation injury, lung blast injury, pulmonary barotrauma, delayed hemopneumothorax, lung injury associated sepsis, ventilator-induced lung injury and ischemia-reperfusion lung injury. It is also compiled with many clinical typical cases, full data and series of pictures. It persists in combining theory and practice, and highlights practical application to reflect the theoretical value. It is very suitable for the medical teaching and can also be used as a reference book for medical doctoral students, postgraduates, and medical trainees receiving continuing education from critical care medicine, burn & trauma surgery, and emergency medicine. It also aims at bringing more clinicians' attention to burn-/trauma-induced lung injury, making them familiar with the relevant theories and clinical diagnose; guiding the treatment of burn and trauma associated lung injury and improving the prognosis and life quality of patients; stimulating more clinicians and researchers to further explore the pathological mechanism and new treatments of burn and trauma associated lung injury. Editor Zhao-fan Xia is a Professor and Director Department of Burn Surgery, Changhai Hospital, Shanghai, China. Professor Xia is the Academician of Chinese Academy of Engineering.

Note: Inhalation injury -- Primary Blast Lung injury -- Pulmonary barotrauma -- Delayed Hemopneumothorax -- Sepsis Related Lung Injury (SRLI) -- Ventilator-Associated Lung Injury (VALI) -- Ischemia-reperfusion and Oxidative Stress Induced Lung Injury -- Pulmonary Enbolism -- Pulmonary Infection -- Modes and Strategies of Mechanical Ventilation for burn and trauma associated lung injury -- Potential Therapy.

In: Springer Nature eBook

Additional Edition: Printed edition: ISBN 9789811570544

Additional Edition: Printed edition: ISBN 9789811570551

Language: English

DOI: 10.1007/978-981-15-7056-8

URL: https://doi.org/10.1007/978-981-15-7056-8