UID:
almahu_9949251227302882
Format:
1 online resource (345 p.)
Edition:
1st ed. 1999.
ISBN:
1-61737-039-8
,
1-280-83050-6
,
9786610830503
,
1-59259-259-7
Series Statement:
Methods in Molecular Biology, 94
Content:
DNA Topoisomerase Protocols, I: DNA Topology and Enzymes brings together an unprecedented collection of cutting-edge experimental protocols for investigating DNA structure, topology, and DNA topoisomerase function. Described by expert experimentalists who have perfected the techniques, these unfailingly reproducible methods utilize new approaches to study changes in DNA topology (linking number, knotting, catenation, and relaxation) and DNA structure (bending), as well as to assess chromosome structure through FLP-mediated recombination and to analyze bacterial nucleoid structure. State-of-the-art protocols also detail the expression and purification of bacterial, viral, and eukaryotic DNA topoisomerases from a variety of sources, including baculovirus, and bacterial, yeast and mammalian expression systems. A companion volume, DNA Topoisomerase Protocols, v. 2: Enzymology and Drugs, provides detailed protocols to study the catalytic activities of DNA topoisomerases, as well as their specific interactions with topoisomerase-targeted antitumor and antibacterial drugs. DNA Topoisomerase Protocols, I: DNA Topology and Enzymes will be of immense value to the many basic scientists and clinicians who want better to understand and to exploit proficiently the wealth of recent discoveries about chromatin structure and its relation to gene expression, about DNA topoisomerases as the targets of antitumor and antibacterial agents, and about DNA repair.
Note:
Description based upon print version of record.
,
to DNA Topoisomerases -- Resolution of DNA Molecules by One-Dimensional Agarose-Gel Electrophoresis -- Two-Dimensional Agarose-Gel Electrophoresis of DNA Topoisomers -- Analysis of Altered DNA Structures -- Purification of Supercoiled Plasmid DNA -- Purification and Use of DNA Minicircles with Different Linking Numbers -- Isolation of Kinetoplast DNA -- Isolation of Knotted DNA from Coliphage P4 -- Analysis of DNA Knots and Catenanes by Agarose-Gel Electrophoresis -- Sedimentation Analysis of Bacterial Nucleoid Structure -- Coating DNA with RecA Protein to Distinguish DNA Path by Electron Microscopy -- Methods for Analyzing DNA Bending -- Formation of Extrachromosomal DNA Rings in Saccharomyces cerevisiae Using Site-Specific Recombination -- Overexpression and Purification of Bacterial DNA Gyrase -- Overexpression and Purification of Bacterial DNA Topoisomerase I -- Overexpression and Purification of Escherichia coliDNA Topoisomerase III -- Overexpression and Purification of Bacterial Topoisomerase IV -- Purification of the Bacteriophage T4 Type II DNA Topoisomerase -- Overexpression and Purification of DNA Topoisomerase I from Yeast -- Overexpression and Purification of Saccharomyces cerevisiae DNA Topoisomerase II from Yeast -- Purification of DNA Topoisomerase II from Drosophila melanogaster -- Purification of DNA Topoisomerase I from Human Placenta -- Purification of Baculovirus-Expressed Human DNA Topoisomerase I -- Rapid Purification of DNA Topoisomerase II Containing a Hexahistidine Tag by Metal Ion Affinity Chromatography -- Metabolic Labeling, Immunoprecipitation, and Two-Dimensional Tryptic Phosphopeptide Mapping of Human Topoisomerase II -- Immunoblot Analysis and Band Depletion Assays -- Visualization of DNA Topoisomerases by Electron Microscopy -- Appendix: Compendium of DNA Topoisomerase Sequences.
,
English
Additional Edition:
ISBN 0-585-24399-9
Additional Edition:
ISBN 0-89603-444-5
Language:
English
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