In:
The International Journal of Artificial Organs, SAGE Publications, Vol. 17, No. 10 ( 1994-10), p. 554-558
Abstract:
Isolated pig liver cells cultured using a perfusion technique were analyzed over 39 days to test their ability to change the perfusate α-keto acid profile. While the pyruvate concentration in the culture medium decreased as of the first day, the α-ketoglutarate (KG), α-ketoisocaproate (KIC), α-ketoisovalerate (KIV) and α-ketoβ-methyl-n-valerate (KMV) were synthesized immediately and released by the liver cells. The metabolic capacity of the cell culture system increased up to day 10, decreased during the following 5 days and reached a steady state beyond day 15, which was maintained for at least 30 days. The branched chain α-keto acid release, in particular α-ketoisocaproate, reflects an effective transamination capacity of the newly developed culture system and shows an intact protein biosynthesis for at least 30 days in vitro.
Type of Medium:
Online Resource
ISSN:
0391-3988
,
1724-6040
DOI:
10.1177/039139889401701008
Language:
English
Publisher:
SAGE Publications
Publication Date:
1994
detail.hit.zdb_id:
1474999-3
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