In:
Chemie in unserer Zeit, Wiley, Vol. 53, No. 5 ( 2019-10), p. 292-299
Abstract:
Metal compounds are ubiquitously distributed in the earth crust and indispensable for many technological applications. In addition, trace elements are essential for all biological systems. Nevertheless, several metal compounds are toxic and even carcinogenic, and in case of trace elements, a strict cellular homeostasis is highly required. One important aspect concerns the role of metal ions in maintaining genomic stability. Thus, for example, zinc‐binding structures in DNA processing proteins contribute greatly to the fidelity of DNA repair and tumor suppressor processes. On the other hand, exactly these structures appear to be very sensitive targets towards toxic metal ions. However, also trace elements may become toxic if cellular levels are highly exceeded due to elevated uptake levels and/or non‐physiological exposure routes such as inhalation. One very promising approach to elucidate modes of action consists in the application of a recently established high‐throughput‐RT‐qPCR method, enabling the parallel investigation of the impact on the expression 95 genes, selected based on their role for genomic stability, in 96 samples. This system allows not only the identification of toxicological “fingerprints”, but also the establishment of dose‐response‐relationships as well as comparative investigations of different metal species. Additionally, it enables detailed mechanistic studies, for example with respect to metal‐based nanoparticles. All these aspects are important requirements for science‐based risk‐benefit analyses.
Type of Medium:
Online Resource
ISSN:
0009-2851
,
1521-3781
DOI:
10.1002/ciuz.201900835
Language:
German
Publisher:
Wiley
Publication Date:
2019
detail.hit.zdb_id:
2006650-8
detail.hit.zdb_id:
6904-8
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