In:
cclm, Walter de Gruyter GmbH, Vol. 36, No. 10 ( 1998-10-13), p. 797-801
Abstract:
Serum levels of Interleukin-6 (IL-6), a proinflammatory cytokine, are increased in early stages of inflammatory diseases such as infection and sepsis. Assay systems which permit its measurement within a few hours and as a single measurement have not been reported so far. We therefore evaluated a now commercially available automated method for IL-6 measurement on the Cobas Core ® immunological analyzer (Roche Diagnostic Systems) which enables single IL-6 measurement within about 1 hour. The automated assay correlates well with an established, manual microtiter plate assay (Biosource GmbH) which uses the same antibodies and reagents (r=0.98). Accuracy of the automated method was established by adding known amounts of IL-6 international reference preparation. Recovery of the international standard was in the range of 92–104 %. The automated assay had a precision of singletons below 6% and was linear up to 2800 pg/ml. This automated assay provides a suitable, convenient and time saving method for measurement of IL-6 serum levels in the routine clinical laboratory.
Type of Medium:
Online Resource
ISSN:
1434-6621
DOI:
10.1515/CCLM.1998.141
Language:
English
Publisher:
Walter de Gruyter GmbH
Publication Date:
1998
detail.hit.zdb_id:
1492732-9
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