In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 64, No. Pt_10 ( 2014-10-01), p. 3485-3491
Abstract:
A previously isolated exoelectrogenic bacterium, strain SD-1 T , was further characterized and identified as a representative of a novel species of the genus Geobacter . Strain SD-1 T was Gram-negative, aerotolerant, anaerobic, non-spore-forming, non-fermentative and non-motile. Cells were short, curved rods (0.8–1.3 µm long and 0.3 µm in diameter). Growth of strain SD-1 T was observed at 15–42 °C and pH 6.0–8.5, with optimal growth at 30–35 °C and pH 7. Analysis of 16S rRNA gene sequences indicated that the isolate was a member of the genus Geobacter , with the closest known relative being Geobacter sulfurreducens PCA T (98 % similarity). Similar to other members of the genus Geobacter , strain SD-1 T used soluble or insoluble Fe(III) as the sole electron acceptor coupled with the oxidation of acetate. However, SD-1 T could not reduce fumarate as an electron acceptor with acetate oxidization, which is an important physiological trait for G. sulfurreducens . Moreover, SD-1 T could grow in media containing as much as 3 % NaCl, while G. sulfurreducens PCA T can tolerate just half this concentration, and this difference in salt tolerance was even more obvious when cultivated in bioelectrochemical systems. DNA–DNA hybridization analysis of strain SD-1 T and its closest relative, G. sulfurreducens ATCC 51573 T , showed a relatedness of 61.6 %. The DNA G+C content of strain SD-1 T was 58.9 mol%. Thus, on the basis of these characteristics, strain SD-1 T was not assigned to G. sulfurreducens , and was instead classified in the genus Geobacter as a representative of a novel species. The name Geobacter anodireducens sp. nov. is proposed, with the type strain SD-1 T ( = CGMCC 1.12536 T = KCTC 4672 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.061598-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2014
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
Bookmarklink