In:
Zeitschrift für Naturforschung C, Walter de Gruyter GmbH, Vol. 41, No. 11-12 ( 1986-12-1), p. 1052-1056
Abstract:
prepared from leaves of various higher plants were stained with the specific DNA fluorochrome DAPI and measured with a pulse cytophotometer. DNA distribution curves of protoplasts showed DNA histograms like assays of animal cells stained with the same dye solution. The percentage of fractions of cell populations in G 0 /G 1 -, S- and G 2 + M-phase of the cell cycle could be calculated. A simple method of protoplasts preparation and of flow cytometric estimation of DAPI stained plant protoplasts is described. This method allows cytogenetic and cytokinetic studies of cells from plant material and may be used for fluorescence activated cell sorting.
Type of Medium:
Online Resource
ISSN:
1865-7125
,
0939-5075
DOI:
10.1515/znc-1986-11-1217
Language:
English
Publisher:
Walter de Gruyter GmbH
Publication Date:
1986
detail.hit.zdb_id:
2078107-6
SSG:
12
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