In:
The Journal of Immunology, The American Association of Immunologists, Vol. 171, No. 2 ( 2003-07-15), p. 1102-1108
Abstract:
This study had two aims: 1) to determine whether there are differences between atopic dermatitis (AD) patients and healthy subjects in staphylococcal superantigen (SsAg)-induced CD4+ T cell activation, cytokine production, chemokine receptor expression, and apoptosis; and 2) to investigate the effect of IL-4 on SsAg-induced apoptosis. By using immunofluorescence and annexin V staining, we analyzed PBMC with or without staphylococcal enterotoxin B (SEB) stimulation in the presence or absence of rIL-4 or anti-IL-4-neutralizing Abs in 15 healthy subjects and 27 AD patients. We found that SEB preferentially induced production of Th1 cytokine in SEB-reactive (TCRVβ3+ or Vβ12+ or Vβ17+) CD4+ T cells from healthy subjects and Th2 cytokine in those from AD patients. SEB induced up-regulation of CXCR3+ cells in SEB-reactive CD4+ T cells from healthy subjects and CCR4+ cells in those from AD patients. SEB-reactive CD4+ T cells from AD patients were more resistant to SEB-induced apoptosis than those from healthy subjects. There was no significant difference between AD and healthy subjects in SEB-induced activation of CD4+ T cells. CXCR3+ CD4+ T cells were more susceptible to SEB-induced apoptosis than CCR4+ CD4+ T cells in healthy subjects. Exogenously added IL-4 inhibited SEB-induced apoptosis of SEB-reactive CD4+ and CXCR3+ CD4+ T cells but not of CCR4+ CD4+ T cells in healthy subjects. Inhibition of endogenous IL-4 increased SEB-induced apoptosis of SEB-reactive CD4+ T cells from AD patients. These results might provide new clues to the mechanism that SsAgs contribute to the persistence and exacerbation of allergic skin inflammation in AD.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.171.2.1102
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
2003
detail.hit.zdb_id:
1475085-5
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