In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 62, No. Pt_4 ( 2012-04-01), p. 900-905
Abstract:
A Gram-positive, non-motile actinobacterium, designated YIM 65754 T , was isolated from the stem of Artemisia annua L., collected from Yunnan province, south-west China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 65754 T comprised an evolutionary lineage within the genus Rhodococcus . The isolate clustered with Rhodococcus pyridinivorans PDB9 T , Rhodococcus gordoniae W 4937 T and Rhodococcus rhodochrous DSM 43241 T , with which it shared 98.4, 97.9 and 97.8 % 16S rRNA gene sequence similarities, respectively. However, DNA–DNA relatedness demonstrated that strain YIM 65754 T was distinct from its closest phylogenetic neighbours. The cell-wall peptidoglycan contained meso -diaminopimelic acid, arabinose, galactose, mannose and glucose (cell-wall chemotype IV). The major menaquinone was MK-8(H 2 ) and the predominant fatty acids were C 16 : 0 (27.83 %), iso-C 15 : 0 2-OH and/or C 16 : 1 ω7 c (20.21 %) and 10-methyl C 18 : 0 (17.50 %). The DNA G+C content was 66.2 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, the isolate represents a novel species of the genus Rhodococcus , for which the name Rhodococcus artemisiae sp. nov. is proposed; the type strain is YIM 65754 T ( = CCTCC AA 209042 T = DSM 45380 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.031930-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2012
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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