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  • Microform  (4)
Type of Material
  • Microform  (4)
Type of Publication
Consortium
Language
  • 1
    UID:
    (DE-603)272414115
    Format: 150 S.
    Edition: Mikrofilm-Ausg. Ann Arbor, Mich. Univ. Microfilms Internat. 1982 1 Mikrofilm : 35 mm [Mikrofilm-Ausg.]
    Content: The visual patterns of the process of enzymatic cellulose degradation were established at the macromolecular level utilizing high resolution negative staining electron microscopic techniques. The employment of ultrathin carbon films as a support matrix for negative staining allowed observations of the fine structure of cellulosic ribbons produced by the Gram-negative bacterium Acetobacter xylinum. Visualization of cellulose microfibrils and bundles of microfibrils of various sizes suggests that A. xylinum cellulose ribbons are comprised of a heirarchical arrangement of crystalline cellulose structures. Individual enzyme molecules from the cellulase enzyme system of the imperfect fungus Trichoderma reesei QM9414 were imaged, as well as the binding of the enzymes to the surface of the cellulose substrate, and the subsequent fine structural changes which occur in cellulose structure during enzymatic degradation. After short exposures to cellulase enzyme solutions, ribbon surfaces were obscured by bound particles with diameters ranging from 3 to 7 nm with a mean diameter of 5.4 (+OR-) 0.2 nm. Negative staining of dilute cellulase enzyme solutions revealed spherical particles with diameters ranging from 3 to 7 nm with a mean diameter of 5.4 (+OR-) 0.1 nm. Evidence was presented that the particles observed in the solution and bound to the cellulose surfaces are, in fact, cellulase enzymes. Visual patterns of the process of cellulose degradation were established. Enzymes were initially observed bound to the cellulose ribbon. Within 10 min, the ribbon was split along its long axis into bundles of microfibrils which were subsequently thinned until they were completely dissolved within 30 min. The actions of purified components of the enzyme system were examined and compared with the synergistic action of the complete system. Incubations with purified components of the cellulase enzyme system produced less dramatic changes in ribbon structure. Purified 1,4-(beta)-D-glucan cellobiohydrolase I(D) (EC 3.2.1.91) produced no visible change in cellulose structure. Purified endo-1,4-(beta)-D-glucanase IV (EC 3.2.1.4) produced some splaying of ribbons into microfibril bundles. When purified enzymes were present simultaneously in an incubation admixture, cellulose was completely degraded in the normal manner within 30 min. Sequential incubation in purified Endoglucanase IV and Cellobiohydrolase I(D) illustrated the necessity of simultaneous presence of these two enzymes before synergistic degradation of cellulose is possible. The observed actions of the cellulose enzyme system, purified components of the system applied singly or sequentially, or admixtures of the purified components, were consistent with the existing biochemical data on the mode of action of the cellulase enzyme system.
    Note: Chapel Hill, NC, Univ. of North Carolina, Diss., 1981 , Mikrofilm-Ausg.:
    Language: English
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  • 2
    UID:
    (DE-603)272414123
    Format: 295 S.
    Edition: Mikrofilm-Ausg. Ann Arbor, Mich. Univ. Microfilms Internat. 1985 1 Mikrofilm : 35 mm [Mikrofilm-Ausg.]
    Content: Marine parks and reserves are observed and evaluated as management techniques for coral reefs. Coral reef depletion, overexploitation of reef resources, and destructive fishing, indicate the need for management. These problems are contrasted with the benefits provided by coral reefs. Since reefs are among the most productive ecosystems and supply a significant quality of edible protein to island and coastal people, sustainable yield management is an important long-term goal. Seven reserve management and two control areas in the Philippines are discussed in detail and compared with two similar management areas in Indonesia and two in Malaysia. This study undertakes three objectives: (1) to document the status of various reefs in the Philippines, Indonesia and Malaysia in terms of reef biology, management approaches, patterns of human exploitation, and the larger environmental settings of the reefs; (2) to examine the effects of formal management schemes, human exploitation, and general setting on the reef environments; and (3) to investigate generalities between sites in terms of environmental conditions, management and use by humans. Coral reef parameters used to evaluate reef condition, include: substrate cover; density of coral genera, Acanthaster, and Tridacna; chaetodontid diversity; topographic relief; and noticeable damage. Sites are ranked in terms of reef quality and are compared as to remoteness, human exploitation, destructive uses and management types. Municipal and national management approaches are contrasted and the effects of local education, scientific and tourist interests are noted at each site. All sites where some form of management exists, except one, show an apparent positive impact and potential for sustainability. Control sites and those proposed for management without protection show some form of degradation. Management recommendations emphasize education and participation of local communities in reserve planning and implementation. Integration of national or municipal reserve management plans with local resource use patterns and needs is discussed. "Core" and "buffer" zones are shown to be effective for integrating ecological reserve design constraints with traditional fishing, tourism, scientific research and environmental education. Implementation or a core reserve area of sanctuary zone is shown to (1) maintain species richness of fishes; (2) provide undisturbed breeding grounds; and (3) export fish biomass. These benefits are associated with acceptance of reserve management schemes by local residents dependent on reef resources and by rational policy makers.
    Note: Honolulu, Hawai, Univ. of Hawaii, Diss., 1984 , Mikrofilm-Ausg.:
    Language: English
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  • 3
    UID:
    (DE-627)01475472X
    Format: 142 S
    Note: North Carolina State Univ., Diss
    Language: Undetermined
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  • 4
    UID:
    (DE-605)HT007160036
    Format: 10 S. : Ill., graph. Darst.
    Edition: [Mikrofiche-Ausg.]
    Note: Mikrofiche-Ausg.: Springfield, VA.: NTIS, [19]93. 1 Mikrofiche
    Language: Undetermined
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