In:
MicrobiologyOpen, Wiley, Vol. 6, No. 5 ( 2017-10)
Abstract:
Two‐component systems in Acinetobacter baumannii are associated with its virulence, drug resistance, motility, biofilm formation, and other characteristics. In this study, we used Rec Ab , a genetic engineering method, to investigate the function of A1S_2811 in A. baumannii strain ATCC 17978. A1S_2811, a hypothetical hybrid sensor histidine kinase/response regulator, has four histidine‐containing phosphotransfer domains, a CheA‐like regulatory domain, and a CheY‐like receiver domain at its C terminus. Compared with the ATCC 17978 strain, both surface motility and biofilm formation at the gas–liquid interface decreased significantly in the A1S_2811 knock‐out strain. The number of pilus‐like structures and the amount of extrapolymeric substances on the cell surface also decreased in the A1S_2811 null strain. Transcription of abaI , which encodes an N ‐acylhomoserine lactone synthase in A. baumannii , decreased significantly in the A1S_2811 null strain, and supplementation with synthetic N ‐(3‐oxodecanoyl) homoserine‐ l ‐lactone rescued the surface motility and biofilm formation phenotype in the null mutant. We speculate that A1S_2811 regulates surface motility and biofilm formation, not by regulating type IV pili‐associated genes expression, but by regulating the chaperone/usher pili‐associated csuA/ ABCDE operon and the AbaI‐dependent quorum‐sensing pathway‐associated A1S_0112‐0119 operon instead.
Type of Medium:
Online Resource
ISSN:
2045-8827
,
2045-8827
DOI:
10.1002/mbo3.2017.6.issue-5
Language:
English
Publisher:
Wiley
Publication Date:
2017
detail.hit.zdb_id:
2661368-2
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