In:
Digestion, S. Karger AG, Vol. 103, No. 6 ( 2022), p. 451-461
Abstract:
〈 b 〉 〈 i 〉 Introduction: 〈 /i 〉 〈 /b 〉 〈 i 〉 Fusobacterium 〈 /i 〉 and several bacteria are reported to be associated with colorectal cancer (CRC). However, their relationship and whether they cause CRC or are just adapted to the cancerous environment is not known. We approached this subject by investigating the correlation and distribution of the bacteria throughout the colon in patients with CRC and elucidated the relationship between microbiota and CRC. 〈 b 〉 〈 i 〉 Methods: 〈 /i 〉 〈 /b 〉 Twenty-five patients with CRC who underwent colonoscopy for endoscopic submucosal dissection or surgery were prospectively enrolled. Fecal samples were taken before bowel preparation, and mucosal samples were collected from three sites (tumor surface, tumor-adjacent mucosa, and cecum) during colonoscopy using a cytology brush. The microbiota was identified and analyzed by sequencing of the 16S rRNA gene of the V3–V4 region. We evaluated the correlation between the bacteria based on network analysis and the distribution of 〈 i 〉 Fusobacterium 〈 /i 〉 in the colon. 〈 b 〉 〈 i 〉 Results: 〈 /i 〉 〈 /b 〉 A network consisting of many bacteria was found in all sites; especially, oral origin bacteria including 〈 i 〉 Fusobacterium 〈 /i 〉 formed a positively correlated network on tumor surface. 〈 i 〉 Streptococcus 〈 /i 〉 showed a significantly higher relative abundance on tumor surface than in feces. The relative abundance of 〈 i 〉 Fusobacterium 〈 /i 〉 had significant positive correlations between tumor surface and feces, tumor-adjacent mucosa, and cecum. 〈 b 〉 〈 i 〉 Conclusion: 〈 /i 〉 〈 /b 〉 In patients with CRC, many bacteria were correlated with each other, and 〈 i 〉 Fusobacterium 〈 /i 〉 and oral origin bacteria formed a positively correlated network on tumor surface. 〈 i 〉 Fusobacterium 〈 /i 〉 was equally distributed on tumor surface and throughout the lumen and mucus in the colon. In the colon where 〈 i 〉 Fusobacterium 〈 /i 〉 is widely distributed, 〈 i 〉 Fusobacterium 〈 /i 〉 would adhere to the tumor surface and be correlated with oral origin bacteria to make a microenvironment that is favorable for CRC.
Type of Medium:
Online Resource
ISSN:
0012-2823
,
1421-9867
Language:
English
Publisher:
S. Karger AG
Publication Date:
2022
detail.hit.zdb_id:
1482218-0
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