In:
Journal of Biomaterials and Tissue Engineering, American Scientific Publishers, Vol. 12, No. 6 ( 2022-06-01), p. 1182-1188
Abstract:
In age-related macular degeneration (AMD), hypoxia causes abnormal changes in tissue metabolism and retinal pigment epithelium (RPE). However, the changes in cell function and intracellular molecules in RPE cells induced by hypoxia are not fully understood. In our study, cell viability,
apoptosis, oxidative stress and changes in intracellular molecules at different time points were evaluated in ARPE-19 cells using a hypoxia model. A short period of mild hypoxia stimulated cell proliferation and apoptosis was induced as the period of hypoxia increased and the oxygen concentration decreased. As hypoxia worsened, the mitochondrial reactive oxygen species (ROS) accumulation was often accompanied with increased oxidative stress, increased intracellular HIF-1 α accumulation and VEGF secretion to induce neovascularization. Additionally, p-ERK1 and ERK2 level
was increased and p-ERK2 was decreased during hypoxia. In conclusion, our results show that the changes in the RPE is a process that depends on the degree of hypoxia and exposure time.
Type of Medium:
Online Resource
ISSN:
2157-9083
DOI:
10.1166/jbt.2022.3024
Language:
English
Publisher:
American Scientific Publishers
Publication Date:
2022
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