In:
Developmental Neuroscience, S. Karger AG, Vol. 26, No. 1 ( 2004), p. 68-76
Abstract:
We screened for factors upregulating glial fibrillary acidic protein (GFAP) promoter activity by functional cloning with an immature astrocyte cell line (HB108-10) harboring a GFAP- 〈 i 〉 lacZ 〈 /i 〉 construct. One cDNA clone that repeatedly upregulated 〈 i 〉 lacZ 〈 /i 〉 expression encoded cystatin C (CysC), a cysteine protease inhibitor. TGF-β induced CysC and GFAP expression in AP-16 cells, an astrocyte progenitor-like cell line expressing GLAST (a glutamate transporter subtype specifically expressed in immature astrocytes). CysC gene expression started earlier than that of GFAP in the mouse forebrain. It started in the ventricular zone at a similar period as (or slightly after) GLAST expression, but before GFAP expression. Although previous data showed that CysC is involved in the maintenance of adult neural stem cells, our data indicate that it is involved in astrocyte differentiation during mouse brain development.
Type of Medium:
Online Resource
ISSN:
0378-5866
,
1421-9859
Language:
English
Publisher:
S. Karger AG
Publication Date:
2004
detail.hit.zdb_id:
1482201-5
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