In:
Pharmacology, S. Karger AG, Vol. 88, No. 5-6 ( 2011), p. 327-332
Abstract:
〈 i 〉 Background/Aims: 〈 /i 〉 Long-term macrolide therapy is an effective treatment for chronic sinusitis and diffuse panbronchiolitis. However, long-term use of macrolides may promote the growth of drug-resistant bacteria; therefore, development of macrolides with no antibacterial action is desirable. A new erythromycin (EM) derivative, (8R,9S)- 8,9-dihydro-6,9-epoxy-8,9-anhydropseudoerythromycin A (EM900), does not possess antibacterial action. 〈 i 〉 Methods: 〈 /i 〉 To determine whether EM900 induced a clinically relevant anti-inflammatory response and repressed mucin gene expression in cells derived from human airway epithelia, we assessed the effects of EM900 on IL-1β-induced inflammatory cytokines in A549 cells and 〈 i 〉 MUC5AC 〈 /i 〉 gene expression in HM3-MUC5AC cells. We also investigated the effects of EM900 on IL-1β-induced NF-ĸB activation. We performed reporter gene assays and quantitative PCR in A549 and HM3-MUC5AC cells. 〈 i 〉 Results: 〈 /i 〉 Both EM and EM900 suppressed IL-1β-induced 〈 i 〉 IL-8 〈 /i 〉 expression in A549 cells. EM900 also suppressed IL-1β-induced 〈 i 〉 IL-1 〈 /i 〉 β and 〈 i 〉 TNF- 〈 /i 〉 α expression in A549 cells. EM900 inhibited IL-1β-induced 〈 i 〉 MUC5AC 〈 /i 〉 expression in HM3-MUC5AC cells. Both EM and EM900 suppressed IL-1β-induced NF-ĸB activation in A549 cells. 〈 i 〉 Conclusion: 〈 /i 〉 This study demonstrated that EM900 suppressed the induction of inflammatory cytokines and 〈 i 〉 MUC5AC 〈 /i 〉 gene expression in cells derived from human airway epithelia, and our findings indicate that these effects may be mediated by the suppression of NF-ĸB activation.
Type of Medium:
Online Resource
ISSN:
0031-7012
,
1423-0313
Language:
English
Publisher:
S. Karger AG
Publication Date:
2011
detail.hit.zdb_id:
1483550-2
SSG:
15,3
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