In:
Histopathology, Wiley, Vol. 70, No. 3 ( 2017-02), p. 375-384
Abstract:
The aims of this study were to clarify the usefulness of immunohistochemistry in the differential diagnosis of epithelioid mesothelioma with a solid growth pattern [solid epithelioid mesothelioma ( SEM )] and poorly differentiated squamous cell carcinoma ( PDSCC ), and to confirm the validity of a specific type of antibody panel. Additionally, we aimed to clarify the pitfalls of immunohistochemical analyses. Methods and results Formalin‐fixed paraffin‐embedded specimens from 36 cases of SEM and 38 cases of PDSCC were immunohistochemically examined for calretinin, podoplanin (D2‐40), Wilms’ tumour gene product ( WT 1), cytokeratin ( CK ) 5/6, p40, p63, carcinoembryonic antigen ( CEA ), epithelial‐related antigen ( MOC 31), claudin‐4, thyroid transcription factor‐1 ( TTF ‐1), and napsin A. WT 1 showed the highest diagnostic accuracy (85.1%) as a mesothelial marker, and CEA , p40 and claudin‐4 showed higher diagnostic accuracies (95.9%, 94.6%, and 93.2%, respectively) as carcinoma markers. Calretinin (diagnostic accuracy: 75.7%), D2‐40 (diagnostic accuracy: 67.6%), CK 5/6 (diagnostic accuracy: 63.5%), TTF ‐1 (diagnostic accuracy: 55.4%) and napsin A (diagnostic accuracy: 52.7%) could not differentiate between SEM and PDSCC . Among these markers, the combination of calretinin and WT 1 showed the highest diagnostic accuracy (86.5%) as a positive marker, and the combination of p40 and CEA showed the highest diagnostic accuracy (97.3%) as a negative marker. The combination of CEA and claudin‐4 also showed relatively high diagnostic accuracy (94.6%) as a negative marker. Conclusions We recommend the combination of WT 1 and calretinin as a positive maker, and the combination of CEA and claudin‐4 as a negative marker, for differential diagnoses of SEM and PDSCC .
Type of Medium:
Online Resource
ISSN:
0309-0167
,
1365-2559
DOI:
10.1111/his.2017.70.issue-3
Language:
English
Publisher:
Wiley
Publication Date:
2017
detail.hit.zdb_id:
2006447-0
Bookmarklink