In:
Bulletin of the Korean Chemical Society, Wiley, Vol. 38, No. 7 ( 2017-07), p. 744-750
Abstract:
Liposomes have demonstrated great potentials as protein carriers in various pharmaceutical applications. However, low loading amount of proteins in liposomes have been a major challenge for maximizing the therapeutics potential of the proteins. We thus aimed to enhance the loading amount of a model protein, lysozyme, in liposomes by controlling key experimental variables of a reverse phase evaporation method. The loading amount of lysozyme in liposomes was evaluated with changing type of organic solvents used, weight ratio of lysozyme/phosphatidylcholine, and volume ratio of aqueous to organic phase along with particle characterization before and after freeze‐drying procedure. As a result, the loading amount of lysozyme in liposomes was considerably enhanced and the physical stability of the liposomes was maintained without any significant changes in the particle characteristics for 7 days. The findings of this study would be useful for highly efficient loading of protein therapeutics in liposomes, leading to improved therapeutic effects of the drugs.
Type of Medium:
Online Resource
ISSN:
1229-5949
,
1229-5949
DOI:
10.1002/bkcs.2017.38.issue-7
Language:
English
Publisher:
Wiley
Publication Date:
2017
detail.hit.zdb_id:
2056474-0
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