In:
Intervirology, S. Karger AG, Vol. 55, No. 1 ( 2012), p. 21-28
Abstract:
〈 i 〉 Bombyx mori 〈 /i 〉 densonucleosis virus ( 〈 i 〉 Bm 〈 /i 〉 DNV) is one of the most disastrous viruses in cocoon production. Silkworm resistance to 〈 i 〉 Bm 〈 /i 〉 DNV has been examined previously using a number of traditional biochemical and molecular techniques. In this study, a near isogenic line, BC 〈 sub 〉 6 〈 /sub 〉 , was constructed to eliminate the difference in inherited background, which has 99.9% identity with the susceptible strain but carries a resistant gene. We utilized a proteomic approach involving two-dimensional differential gel electrophoresis and mass spectrometry to examine changes in the midgut proteins from the susceptible and resistant silkworm larvae infected with 〈 i 〉 Bm 〈 /i 〉 DNV. The protein profiles were compared and 9 differentially expressed proteins were identified by mass spectrometry. In the resistant strains, the heat-shock 70-kDa protein cognate, cytochrome P450, vacuolar ATP synthase subunit B, arginine kinase, vacuolar ATP synthase subunit D and glutathione S-transferase sigma were strongly upregulated and α-tubulin was downregulated. Our results imply that these upregulated genes and the downregulated genes might be involved in 〈 i 〉 B. mori 〈 /i 〉 immune responses against 〈 i 〉 Bm 〈 /i 〉 DNV-Z infection.
Type of Medium:
Online Resource
ISSN:
0300-5526
,
1423-0100
Language:
English
Publisher:
S. Karger AG
Publication Date:
2012
detail.hit.zdb_id:
1482863-7
SSG:
12
Bookmarklink