In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 18, No. 10 ( 2022-10-10), p. e1010820-
Abstract:
Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically devastating diseases affecting the swine industry worldwide. To investigate the role of miRNAs in the infection and susceptibility of PRRS virus (PRRSV), twenty-four miRNA libraries were constructed and sequenced from PRRSV-infected and mock-infected Porcine alveolar macrophages (PAMs) of Meishan, Landrace, Pietrain and Qingping pigs at 9 hours post infection (hpi), 36 hpi, and 60 hpi. The let-7 family miRNAs were significantly differentially expressed between PRRSV-infected and mock-infected PAMs from 4 pig breeds. The let-7 family miRNAs could significantly inhibit PRRSV-2 replication by directly targeting the 3’UTR of the PRRSV-2 genome and porcine IL6 , which plays an important role in PRRSV replication and lung injury. NEAT1 acts as a competing endogenous lncRNA (ceRNA) to upregulate IL6 by attaching let-7 in PAMs. EMSA and ChIP results confirmed that ARID3A could bind to the promoter region of pri-let-7a/let-7f/let-7d gene cluster and inhibit the expression of the let-7 family. Moreover, the NF-κB signaling pathway inhibits the expression of the let-7 family by affecting the nuclear import of ARID3A. The pEGFP-N1-let-7 significantly reduced viral infections and pathological changes in PRRSV-infected piglets. Taken together, NEAT1 / ARID3A /let-7/ IL6 play significant roles in PRRSV-2 infection and may be promising therapeutic targets for PRRS.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1010820
DOI:
10.1371/journal.ppat.1010820.g001
DOI:
10.1371/journal.ppat.1010820.g002
DOI:
10.1371/journal.ppat.1010820.g003
DOI:
10.1371/journal.ppat.1010820.g004
DOI:
10.1371/journal.ppat.1010820.g005
DOI:
10.1371/journal.ppat.1010820.g006
DOI:
10.1371/journal.ppat.1010820.g007
DOI:
10.1371/journal.ppat.1010820.g008
DOI:
10.1371/journal.ppat.1010820.s001
DOI:
10.1371/journal.ppat.1010820.s002
DOI:
10.1371/journal.ppat.1010820.s003
DOI:
10.1371/journal.ppat.1010820.s004
DOI:
10.1371/journal.ppat.1010820.s005
DOI:
10.1371/journal.ppat.1010820.s006
DOI:
10.1371/journal.ppat.1010820.s007
DOI:
10.1371/journal.ppat.1010820.s008
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2205412-1
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