In:
The FEBS Journal, Wiley, Vol. 287, No. 9 ( 2020-05), p. 1886-1898
Abstract:
Protein trans ‐splicing catalyzed by split inteins has increasingly become useful as a protein engineering tool. We solved the 1.0 Å‐resolution crystal structure of a fused variant from the naturally split gp41‐1 intein, previously identified from environmental metagenomic sequence data. The structure of the 125‐residue gp41‐1 intein revealed a compact pseudo‐C2‐symmetry commonly found in the Hedgehog/Intein superfamily with extensive charge–charge interactions between the split N‐ and C‐terminal intein fragments that are common among naturally occurring split inteins. We successfully created orthogonal split inteins by engineering a similar charge network into the same region of a cis ‐splicing intein. This strategy could be applicable for creating novel natural‐like split inteins from other, more prevalent cis ‐splicing inteins. Database Structural data are available in the RCSB Protein Data Bank under the accession number 6QAZ .
Type of Medium:
Online Resource
ISSN:
1742-464X
,
1742-4658
Language:
English
Publisher:
Wiley
Publication Date:
2020
detail.hit.zdb_id:
2172518-4
SSG:
12
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