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  • SAGE Publications  (20)
  • 2010-2014  (20)
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  • SAGE Publications  (20)
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  • 2010-2014  (20)
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  • 1
    Online Resource
    Online Resource
    SAGE Publications ; 2014
    In:  Journal of Cerebral Blood Flow & Metabolism Vol. 34, No. 4 ( 2014-04), p. 660-667
    In: Journal of Cerebral Blood Flow & Metabolism, SAGE Publications, Vol. 34, No. 4 ( 2014-04), p. 660-667
    Abstract: Although the innate immune response to induce postischemic inflammation is considered as an essential step in the progression of cerebral ischemia injury, the role of innate immunity mediator NLRP3 in the pathogenesis of ischemic stroke is unknown. In this study, focal ischemia was induced by middle cerebral artery occlusion in NLRP3 −/− , NOX2 −/− , or wild-type (WT) mice. By magnetic resonance imaging (MRI), Evans blue permeability, and electron microscopic analyses, we found that NLRP3 deficiency ameliorated cerebral injury in mice after ischemic stroke by reducing infarcts and blood–brain barrier (BBB) damage. We further showed that the contribution of NLRP3 to neurovascular damage was associated with an autocrine/paracrine pattern of NLRP3-mediated interleukin-1 β (IL-1 β) release as evidenced by increased brain microvessel endothelial cell permeability and microglia-mediated neurotoxicity. Finally, we found that NOX2 deficiency improved outcomes after ischemic stroke by mediating NLRP3 signaling. This study for the first time shows the contribution of NLRP3 to neurovascular damage and provides direct evidence that NLRP3 as an important target molecule links NOX2-mediated oxidative stress to neurovascular damage in ischemic stroke. Pharmacological targeting of NLRP3-mediated inflammatory response at multiple levels may help design a new approach to develop therapeutic strategies for prevention of deterioration of cerebral function and for the treatment of stroke.
    Type of Medium: Online Resource
    ISSN: 0271-678X , 1559-7016
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2014
    detail.hit.zdb_id: 2039456-1
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  • 2
    Online Resource
    Online Resource
    SAGE Publications ; 2012
    In:  Asian Cardiovascular and Thoracic Annals Vol. 20, No. 3 ( 2012-06), p. 252-256
    In: Asian Cardiovascular and Thoracic Annals, SAGE Publications, Vol. 20, No. 3 ( 2012-06), p. 252-256
    Abstract: We assessed the perioperative pattern of serum ischemia-modified albumin and its role as a myocardial ischemia indicator for early detection of perioperative myocardial infarction in patients undergoing off-pump coronary artery bypass grafting. Venous blood samples were collected from 63 consecutive patients before the operation, immediately after the operation, and at 3, 6, 12, and 24 h postoperatively. Serum ischemia-modified albumin levels were analyzed using an albumin cobalt binding test. The patients were divided into 2 groups retrospectively, according to the occurrence of perioperative myocardial infarction. The serum ischemia-modified albumin levels were compared between groups. The levels peaked immediately after the operation, followed by a gradual regression, but remained elevated during the first 24 h in all patients. The occurrence of perioperative myocardial infarction was identified in 10 patients who had significantly higher ischemia-modified albumin levels at 3 h postoperatively and slower regression rates. Perioperative serum ischemia-modified albumin levels might be helpful in predicting perioperative myocardial infarction.
    Type of Medium: Online Resource
    ISSN: 0218-4923 , 1816-5370
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2012
    detail.hit.zdb_id: 2044527-1
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  • 3
    In: Angiology, SAGE Publications, Vol. 61, No. 5 ( 2010-07), p. 427-436
    Abstract: To assess the effect of intensive statins therapy on the outcome of small-diameter vascular prosthesis, we investigated whether atorvastatin treatment (30 mg/d) could accelerate the re-endothelialization process and improve the patency rate in a canine infrarenal abdominal aorta-expanded polytetrafluoroethylene (ePTFE) bypass model. Furthermore, we also evaluated the effect of atorvastatin on the migratory and adherent capacity of circulating endothelial progenitor cells (EPCs) in vitro. Improved patency was confirmed by Doppler sonography and arteriography. Histological and scanning electron microscopy illustrated enhanced re-endothelialization process. Treatment with atorvastatin enhanced the circulating pool of EPCs with fortified migratory and adherent capacity. Reverse transcriptase—polymerase chain reaction (RT-PCR) analysis showed that atorvastatin treatment increased endothelial nitric oxide synthase (eNOS) and kinase insert domain receptor (KDR) messenger RNA (mRNA) expression in cultured EPCs and neointima. In conclusion, intensive statin therapy could be considered a favorable option to improve small-diameter vascular graft patency.
    Type of Medium: Online Resource
    ISSN: 0003-3197 , 1940-1574
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2010
    detail.hit.zdb_id: 2065911-8
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  • 4
    In: Applied Spectroscopy, SAGE Publications, Vol. 65, No. 6 ( 2011-06), p. 620-626
    Abstract: We very recently reported a new spectroscopic application for expanding the versatility of surface Raman called “shell-isolated nanoparticle-enhanced Raman spectroscopy” or “SHINERS”. The most important and most difficult part of the SHINERS experiment is the effective transfer of the strong electromagnetic field from a gold core through the isolating silica or alumina shell to the probed surface. For this it is essential that the chemically inert dielectric shell be ultrathin (2–5 nm) yet pinhole-free. Herein we describe experimental and theoretical aspects of our SHINERS method in more detail. We provide a protocol for the synthesis and characterization of optimized shell-isolated nanoparticles (SHINs), and we examine the advantages of SHINERS nanoparticles over bare gold nanoparticles. We also present high-quality Raman spectra obtained from gold and platinum single-crystal surfaces in an electrochemical environment by our SHINERS technique. SHINERS is a simple and cost-effective approach that expands the flexibility of surface-enhanced Raman scattering (SERS) for an unprecedented diversity of applications in materials and surface sciences.
    Type of Medium: Online Resource
    ISSN: 0003-7028 , 1943-3530
    RVK:
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2011
    detail.hit.zdb_id: 1474251-2
    SSG: 11
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  • 5
    Online Resource
    Online Resource
    SAGE Publications ; 2013
    In:  Journal of International Medical Research Vol. 41, No. 3 ( 2013-06), p. 743-753
    In: Journal of International Medical Research, SAGE Publications, Vol. 41, No. 3 ( 2013-06), p. 743-753
    Abstract: To investigate relationships between several protein biomarkers and clinical responses to neoadjuvant chemotherapy (NAC) in breast cancer. Methods Tumour tissue samples from female patients with locally advanced breast carcinoma (stages IIA to IIIC), treated with NAC regimens (including 5-fluorouracil, epirubicin, cyclophosphamide and docetaxel, epirubicin, cyclophosphamide) were analysed retrospectively. Immunohistochemical analysis was used to test for protein levels of oestrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor (HER)-2, protein 53 (p53) and γ-synuclein. Relationships between protein biomarkers and responses to NAC were analysed by multivariate logistic regression analysis. Results Data from 154 patients (median age, 51 years; range 27–75 years) were included. Multivariate logistic regression analysis showed that γ-synuclein was an independent predictor of NAC objective response rate, and a statistically significant relationship was observed between NAC regimen, γ-synuclein levels and pathological complete response rate. Conclusions These study findings suggest that γ-synuclein – in combination with other markers such as ER, PR and HER-2 – may serve as a biomarker for response to NAC in breast cancer and warrants further study.
    Type of Medium: Online Resource
    ISSN: 0300-0605 , 1473-2300
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2013
    detail.hit.zdb_id: 2082422-1
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  • 6
    Online Resource
    Online Resource
    SAGE Publications ; 2014
    In:  Experimental Biology and Medicine Vol. 239, No. 3 ( 2014-03), p. 376-384
    In: Experimental Biology and Medicine, SAGE Publications, Vol. 239, No. 3 ( 2014-03), p. 376-384
    Abstract: Myocardial ischemia–reperfusion (MIR) injury is characterized by a rapid increase in cytokines and chemokines and an infiltration of inflammatory cells. Toll-like receptors (TLRs) 2 and 4 mediate these inflammatory responses. Herein we investigated the ability of Sparstolonin B (SsnB), a new selective TLR2/4 antagonist, to inhibit the TLR2/4-mediated inflammatory responses during cardiomyocyte hypoxia–reoxygenation injury as well as the responsible mechanisms. Lactate dehydrogenase (LDH) assay was performed to measure the cytotoxicity of SsnB on H9c2 cardiomyocytes. Quantitative real-time PCR (qRT-PCR) confirmed that TLR2 and TLR4 expression was elevated during hypoxia–reoxygenation, and that their up-regulation in cardiomyocytes was significantly inhibited by SsnB ( P  〈  0.05). Both the mRNA and protein levels of monocyte chemotactic protein-1 and high mobility group box 1 were up-regulated during hypoxia–reoxygenation and were significantly attenuated by SsnB ( P  〈  0.05). Next we found that extracellular signal-regulated kinase 1 or 2 (ERK1/2) and c-Jun NH2-terminal kinase (JNK) signaling pathways were activated during hypoxia–reoxygenation and SsnB significantly inhibited their activation ( P  〈  0.05). Moreover, transwell migration assays revealed that the migration of mouse macrophages to hypoxia–reoxygenation injured cardiomyocytes was significantly reduced by SsnB ( P  〈  0.05). In conclusion, our data indicate that the new selective TLR2 and TLR4 antagonist, SsnB, can substantially attenuate hypoxia–reoxygenation-induced inflammation of cardiomyocytes via inhibiting ERK1/2 and JNK signaling pathways. Accordingly, SsnB has the potential to serve as a therapeutic agent for the prevention of MIR injury.
    Type of Medium: Online Resource
    ISSN: 1535-3702 , 1535-3699
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2014
    detail.hit.zdb_id: 2020856-X
    SSG: 12
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  • 7
    Online Resource
    Online Resource
    SAGE Publications ; 2013
    In:  Hong Kong Journal of Occupational Therapy Vol. 23, No. 2 ( 2013-12), p. 62-68
    In: Hong Kong Journal of Occupational Therapy, SAGE Publications, Vol. 23, No. 2 ( 2013-12), p. 62-68
    Abstract: Although fieldwork experience plays an important role in an occupational therapy (OT) student's choice of future area of practice, studies on the influences of fieldwork on the preference of practice areas remain limited. The goal of the study was to further understand how OT students choose their future areas of practice. Methods: A questionnaire, “The preferred future practice areas of occupational therapy students”, was created by systematic review of the relevant literature and collection of opinions from occupational therapists, students, and faculty. In total, 152 OT students undergoing their Level II fieldwork participated in the survey. Reliability tests, factor analysis tests, and discrimination analysis were carried out. Results The questionnaire demonstrated good to moderate reliability and validity. The factorial analysis indicated that the most influential factors for the preference of future practice areas of senior occupational therapy students are as follows: fieldwork experiences with clinical supervisors, fieldwork experiences with peers, social factors, college courses, and pressure from future work. The discrimination analysis revealed that social factors and college courses are the best factors for discriminating the variable “Choosing physical dysfunction, paediatric, or psychiatric OT as the most preferred future practice area.” Conclusion The choice of future area of practice is proved to be influenced by the experience in fieldwork for OT students. The psychometric properties of the questionnaire used in this study are supported, and it is recommended for use in future studies.
    Type of Medium: Online Resource
    ISSN: 1569-1861 , 1876-4398
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2013
    detail.hit.zdb_id: 2202253-3
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  • 8
    Online Resource
    Online Resource
    SAGE Publications ; 2013
    In:  International Journal of Spray and Combustion Dynamics Vol. 5, No. 3 ( 2013-09), p. 225-242
    In: International Journal of Spray and Combustion Dynamics, SAGE Publications, Vol. 5, No. 3 ( 2013-09), p. 225-242
    Abstract: A fully compressible 3D solver for reacting flows has been developed and applied to investigate a turbulent lifted jet flame in a vitiated coflow by means of direct numerical simulation (DNS) to validate the solver and analyze the flame characteristics. An eighth-order central differencing scheme is used for spatial discretization and a fourth-order Runge-Kutta method is employed for time integration. The DNS results agree well with the experimental measurements for the conditional means of reactive scalars. However, the lift-off height is under predicted. The mean axial velocity develops into a self-similar profile after x/D = 6. The normalized flame index is employed to characterize the combustion regime. It is found that at the flame base the gradients of the reactants are opposed and diffusion combustion is dominant. Further downstream, the contribution of premixed combustion increases and peaks at x/D = 8. Finally, the stabilization process is examined. The turbulent lifted flame is proved to stabilize in the lean mixtures and low scalar dissipation rate regions.
    Type of Medium: Online Resource
    ISSN: 1756-8277 , 1756-8285
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2013
    detail.hit.zdb_id: 2504645-7
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  • 9
    Online Resource
    Online Resource
    SAGE Publications ; 2013
    In:  Advances in Mechanical Engineering Vol. 5 ( 2013-01), p. 357920-
    In: Advances in Mechanical Engineering, SAGE Publications, Vol. 5 ( 2013-01), p. 357920-
    Abstract: In order to improve the accuracy and efficiency of the real-time error compensation, a Fourier Series-Neural Network (FS-NN) based compensation methodology is developed to reduce the thermally induced geometric errors of CNC milling machines under varying conditions. Firstly, an error model is presented based on the principle of Fourier series with fast calculation and higher precision, which is regarded as the error base of original geometric errors. Secondly, the relationships between the slopes related to different error curves and the temperatures of key thermal points are figured out by using neural networks when concerning thermal effects. Then, a combined error model is established which is suitable for reducing axis positioning errors at any thermal status. Finally, a real-time dynamic error compensation system is developed featuring automatic error modeling and multiaxis synchronous compensation. The experimental results prove that the proposed methodology has satisfactory modeling accuracy and robustness to frequently changing working conditions.
    Type of Medium: Online Resource
    ISSN: 1687-8140 , 1687-8140
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2013
    detail.hit.zdb_id: 2501620-9
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  • 10
    Online Resource
    Online Resource
    SAGE Publications ; 2010
    In:  Biological Research For Nursing Vol. 12, No. 1 ( 2010-07), p. 20-27
    In: Biological Research For Nursing, SAGE Publications, Vol. 12, No. 1 ( 2010-07), p. 20-27
    Abstract: The purposes of this prospective, longitudinal study were to examine the relationships between leptin, human chorionic gonadotropin (hCG), cortisol, and psychosocial stress and nausea and vomiting (NV) in women with mild-to-moderate NV throughout pregnancy. Participants comprised 91 pregnant women recruited from prenatal clinics in southern Taiwan. Data analysis using one-way analysis of variance (ANOVA) indicated that leptin, hCG, cortisol levels, and Index of Nausea, Vomiting, and Retching (INVR) scores, but not stress (measured with the Visual Analog Scale, VAS) scores, were significantly different among the three trimesters. The average INVR score and hCG level decreased from the first to third trimesters (p 〈 .0001 for both). The average leptin and cortisol levels increased from the first to third trimesters (p = .001 and p 〈 .0001, respectively). Analysis using mixed models indicated that the INVR scores decreased significantly in a progressive manner through the stages of pregnancy and were significantly lower in the second and third trimesters. Findings reveal that stress/VAS and hCG may both be significantly and independently associated with INVR scores. Future research should examine psychosocial reactions in addition to exploring other biochemical markers related to NV and stress.
    Type of Medium: Online Resource
    ISSN: 1099-8004 , 1552-4175
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2010
    detail.hit.zdb_id: 2070503-7
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