In:
Applied Spectroscopy, SAGE Publications, Vol. 61, No. 11 ( 2007-11), p. 1233-1237
Abstract:
Near-infrared Raman spectroscopy has been used for species identification of pure microbial specimens for more than a decade. More recently, this optical method has been extended to the analysis of specimens containing multiple species. In this report, we demonstrate rapid, reagent-free quantitative analysis of a simplified model of oral plaque containing three oral bacteria species, S. mutans, S. sanguis, and S. gordonii, using near-infrared Raman spectroscopy. Raman spectra were acquired from bacterial mixtures in 200 seconds. A prediction model was calibrated by the partial least squares method and validated by additional samples. On a scale from 0 to 1, relative fractions of each species could be predicted with a root mean square error of 0.07. These results suggest that near-infrared Raman spectroscopy is potentially useful in quantification of microbial mixtures in general and oral plaques in particular.
Type of Medium:
Online Resource
ISSN:
0003-7028
,
1943-3530
DOI:
10.1366/000370207782597021
Language:
English
Publisher:
SAGE Publications
Publication Date:
2007
detail.hit.zdb_id:
1474251-2
SSG:
11
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